Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow KHSRP rightarrow Human KHSRP  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Respiratory AdenoVirus PCR Kit
 • Benzodiazepine (BZO) Antibody
 • Maspin (serpin B5) ELISA Kit
 • SULT1A3 Antibody
 • Pulmonary Surfactant Associated Protein B (SPB) ELISA Kit
 • SUMO2 ELISA Kit
 • E1B protein, large T-antigen Recombinant Protein
 • fibrinogen antiserum (FGA) Antibody
 • CYP1B1 ELISA Kit
 • Acetylcholinesterase (AChE) ELISA Kit
 • Metallothionein-2 (MT2A) Recombinant Protein
 • coxsackievirus A16 (CVA16) antibody (IgG) ELISA Kit
 • VEGFR1 Antibody
 • Tissue Factor (TF) Antibody
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

KHSRP elisa kit :: Human KH-type splicing regulatory protein ELISA Kit

Scan QR to view Datasheet Catalog #    MBS924450
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

KH-type splicing regulatory protein (KHSRP), ELISA Kit

★Popular Item★
 Also Known As   

Human Far upstream element-binding protein 2, KHSRP ELISA Kit

 Product Synonym Names    Human Far upstream element-binding protein 2 (KHSRP) ELISA kit; FBP2; FUBP2; KSRP; MGC99676; FUSE binding protein 2; far upstream element-binding protein 2; KH-type splicing regulatory protein
 Product Gene Name   

KHSRP elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS924450 Testing Data    Testing Data PDF
 OMIM    603445
 3D Structure    ModBase 3D Structure for Q92945
Section Bar
 Species Reactivity    Human
Section Bar
 Specificity    This assay has high sensitivity and excellent specificity for detection of human KHSRP. No significant cross-reactivity or interference between human KHSRP and analogues was observed.
Section Bar
 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    39 pg/ml -2500 pg/ml.
 Sensitivity    The minimum detectable dose of human KHSRP is typically less than 9.75 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Section Bar
 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of KHSRP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for KHSRP purchase    MBS924450 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the KH-type splicing regulatory protein (KHSRP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 39 pg/ml -2500 pg/ml in biological research samples containing KHSRP, with an estimated sensitivity of The minimum detectable dose of human KHSRP is typically less than 9.75 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS924450 kit is based on KHSRP antibody-KHSRP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KHSRP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KHSRP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Product Description specifically for KHSRP elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KHSRP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KHSRP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KHSRP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KHSRP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of KHSRP elisa kit    KHSRP elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS924450. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for KHSRP. It may not necessarily be applicable to this product.
 NCBI GI #    154355000
 NCBI GeneID    8570
 NCBI Accession #    NP_003676.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_003685.2 [Other Products]
Section Bar
 UniProt Primary Accession #    Q92945 [Other Products]
 UniProt Secondary Accession #    O00301; Q59EZ9; Q5U4P6; Q9UNT5; Q9UQH5 [Other Products]
 UniProt Related Accession #    Q92945 [Other Products]
 Molecular Weight    73,115 Da
Table BarTOPTable Bar
 NCBI Official Full Name    far upstream element-binding protein 2
 NCBI Official Synonym Full Names    KH-type splicing regulatory protein
 NCBI Official Symbol    KHSRP [Similar Products]
 NCBI Official Synonym Symbols   
FBP2; KSRP; FUBP2
[Similar Products]
 NCBI Protein Information    far upstream element-binding protein 2; p75; FUSE binding protein 2; FUSE-binding protein 2; KH type-splicing regulatory protein
 UniProt Protein Name    Far upstream element-binding protein 2
 UniProt Synonym Protein Names   
KH type-splicing regulatory protein; KSRP; p75
 Protein Family    Far upstream element-binding protein
 UniProt Gene Name    KHSRP [Similar Products]
 UniProt Synonym Gene Names    FUBP2; FUSE-binding protein 2; KSRP [Similar Products]
 UniProt Entry Name    FUBP2_HUMAN
Section Bar
 NCBI Summary for KHSRP    The KHSRP gene encodes a multifunctional RNA-binding protein implicated in a variety of cellular processes, including transcription, alternative pre-mRNA splicing, and mRNA localization (Min et al., 1997 [PubMed 9136930]; Gherzi et al., 2004 [PubMed 15175153]).[supplied by OMIM, Apr 2010]
Section Bar
 UniProt Comments for KHSRP    KHSRP: an RNA binding and processing protein that binds to the dendritic targeting element and may play a role in mRNA trafficking. Part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. Mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3' untranslated regions, possibly by recruiting degradation machinery to ARE-containing mRNAs. Interacts with APC which is part of the apolipoprotein B mRNA editing enzyme complex. The interaction is not required for activity of the catalytic component, APOBEC1, but may confer stability on the complex.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; membrane; stress granule; cytosol

Molecular Function: mRNA binding; DNA binding

Biological Process: RNA splicing, via transesterification reactions; mRNA transport; transcription, DNA-dependent; regulation of transcription, DNA-dependent; RNA splicing; mRNA catabolic process; gene expression; mRNA processing
Table BarTOPTable Bar
 Research Articles on KHSRP    1. subcellular localization of KSRP is regulated by competing interactions with DDX1 or 14-3-3
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with KHSRP elisa kitPathways associated with KHSRP elisa kit
 Reference Product  PubMed Publications
 FOS elisa kit  >2 publications with KHSRP and FOS
 PARN elisa kit  >1 publications with KHSRP and PARN
 JUN elisa kit  >1 publications with KHSRP and JUN
 Products by Pathway  Pathway Diagram
 Activation Of Genes By ATF4 Pathway antibodies  Activation Of Genes By ATF4 Pathway Diagram
 Destabilization Of MRNA By KSRP Pathway antibodies  Destabilization Of MRNA By KSRP Pathway Diagram
 Diabetes Pathways antibodies  Diabetes Pathways Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 PERK Regulated Gene Expression Pathway antibodies  PERK Regulated Gene Expression Pathway Diagram
 Regulation Of MRNA Stability By Proteins That Bind AU-rich Elements Pathway antibodies  Regulation Of MRNA Stability By Proteins That Bind AU-rich Elements Pathway Diagram
 Unfolded Protein Response Pathway antibodies  Unfolded Protein Response Pathway Diagram
Diseases associated with KHSRP elisa kitOrgans/Tissues associated with KHSRP elisa kit
 Disease Name  Pubmed Publications
 Neurodegenerative Diseases Antibodies  >3 publications with KHSRP and Neurodegenerative Diseases
 Neuroblastoma Antibodies  >2 publications with KHSRP and Neuroblastoma
 Kidney Failure, Chronic Antibodies  >2 publications with KHSRP and Kidney Failure, Chronic
 Genetic Diseases, Inborn Antibodies  >2 publications with KHSRP and Genetic Diseases, Inborn
 Kidney Diseases Antibodies  >2 publications with KHSRP and Kidney Diseases
 Carcinoma Antibodies  >1 publications with KHSRP and Carcinoma
 Congenital Abnormalities Antibodies  >1 publications with KHSRP and Congenital Abnormalities
 Renal Insufficiency Antibodies  >1 publications with KHSRP and Renal Insufficiency
 Mitochondrial Diseases Antibodies  >1 publications with KHSRP and Mitochondrial Diseases
 Carcinoma, Hepatocellular Antibodies  >1 publications with KHSRP and Carcinoma, Hepatocellular
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >6 publications with KHSRP and Liver
 Brain Antibodies  >6 publications with KHSRP and Brain
 Nerve Antibodies  >4 publications with KHSRP and Nerve
 Blood Antibodies  >4 publications with KHSRP and Blood
 Muscle Antibodies  >3 publications with KHSRP and Muscle
 Bone Antibodies  >2 publications with KHSRP and Bone
 Kidney Antibodies  >2 publications with KHSRP and Kidney
 Ganglia Antibodies  >1 publications with KHSRP and Ganglia
 Lung Antibodies  >1 publications with KHSRP and Lung
 Eye Antibodies  >1 publications with KHSRP and Eye
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions