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KGF elisa kit :: Human Keratin growth factor ELISA Kit

Scan QR to view Datasheet Catalog #    MBS721255
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Keratin growth factor (KGF), ELISA Kit

 Also Known As   

Human Keratin growth factor ELISA Kit

 Product Gene Name   

KGF elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    148180
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 Species Reactivity    Human
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    0.5-10ng/mL
 Sensitivity    0.1ng/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of KGF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for KGF purchase    MBS721255 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Keratin growth factor (KGF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.5-10ng/mL in biological research samples containing KGF, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS721255 kit is based on KGF antibody-KGF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KGF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KGF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for KGF elisa kit

   Intended Uses: This KGF ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of KGFin the sample, this KGFELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus KGFconcentration. The concentration of KGFin the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: This KGFenzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for KGF. Standards or samples are then added to the microtiter plate wells and KGFif present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of KGFpresent in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for KGFare added to each well to "sandwich" the KGFimmobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that contain KGFand enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
 Product Categories/Family for KGF elisa kit    Signal Transduction
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 Typical Testing Data/Standard Curve (for reference only) of KGF elisa kit    KGF elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS721255. Click to request current manual
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NCBI/Uniprot data below describe general gene information for KGF. It may not necessarily be applicable to this product.
 NCBI GI #    4503705
 NCBI GeneID    2252
 NCBI Accession #    NP_002000.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_002009.3 [Other Products]
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 UniProt Secondary Accession #    Q6FGV5; Q96FG5; H0YNY5 [Other Products]
 UniProt Related Accession #    P21781 [Other Products]
 Molecular Weight    11,520 Da
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 NCBI Official Full Name    fibroblast growth factor 7
 NCBI Official Synonym Full Names    fibroblast growth factor 7
 NCBI Official Symbol    FGF7 [Similar Products]
 NCBI Official Synonym Symbols   
KGF; HBGF-7
[Similar Products]
 NCBI Protein Information    fibroblast growth factor 7; FGF-7; keratinocyte growth factor; heparin-binding growth factor 7
 UniProt Protein Name    Fibroblast growth factor 7
 UniProt Synonym Protein Names   
Heparin-binding growth factor 7; HBGF-7; Keratinocyte growth factor
 UniProt Gene Name    FGF7 [Similar Products]
 UniProt Synonym Gene Names    KGF; FGF-7; HBGF-7 [Similar Products]
 UniProt Entry Name    FGF7_HUMAN
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 NCBI Summary for KGF    The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis. [provided by RefSeq, Jul 2008]
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 UniProt Comments for KGF    FGF7: Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation. Belongs to the heparin-binding growth factors family.

Protein type: Secreted, signal peptide; Secreted; Cytokine

Chromosomal Location of Human Ortholog: 15q21.2

Cellular Component: Golgi apparatus; extracellular region

Molecular Function: heparin binding; protein binding; growth factor activity; fibroblast growth factor receptor binding; chemoattractant activity

Biological Process: positive regulation of keratinocyte migration; epidermal growth factor receptor signaling pathway; epidermis development; fibroblast growth factor receptor signaling pathway; hair follicle morphogenesis; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; mesenchymal cell proliferation; signal transduction; positive regulation of peptidyl-tyrosine phosphorylation; positive chemotaxis; positive regulation of cell division; actin cytoskeleton reorganization; positive regulation of cell proliferation; response to wounding; insulin receptor signaling pathway; innate immune response; positive regulation of epithelial cell proliferation
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 Research Articles on KGF    1. This paper provides an overview of the knowledge on molecular properties, biological functions and the ecent findings on clinical application of EGF7. [review]
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with KGF elisa kitPathways associated with KGF elisa kit
 Reference Product  PubMed Publications
 FGFR2 elisa kit  >262 publications with KGF and FGFR2
 FGF1 elisa kit  >97 publications with KGF and FGF1
 EGF elisa kit  >71 publications with KGF and EGF
 FGFR1 elisa kit  >62 publications with KGF and FGFR1
 FGFR3 elisa kit  >27 publications with KGF and FGFR3
 FGF4 elisa kit  >20 publications with KGF and FGF4
 FGFR4 elisa kit  >18 publications with KGF and FGFR4
 FGF6 elisa kit  >10 publications with KGF and FGF6
 FGF23 elisa kit  >7 publications with KGF and FGF23
 FGFBP1 elisa kit  >2 publications with KGF and FGFBP1
 Products by Pathway  Pathway Diagram
 Activated Point Mutants Of FGFR2 Pathway antibodies  Activated Point Mutants Of FGFR2 Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Constitutive PI3K/AKT Signaling In Cancer Pathway antibodies  Constitutive PI3K/AKT Signaling In Cancer Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Downstream Signal Transduction Pathway antibodies  Downstream Signal Transduction Pathway Diagram
 Downstream Signaling Events Of B Cell Receptor (BCR) Pathway antibodies  Downstream Signaling Events Of B Cell Receptor (BCR) Pathway Diagram
 Downstream Signaling Of Activated FGFR Pathway antibodies  Downstream Signaling Of Activated FGFR Pathway Diagram
 FGFR Ligand Binding And Activation Pathway antibodies  FGFR Ligand Binding And Activation Pathway Diagram
Diseases associated with KGF elisa kitOrgans/Tissues associated with KGF elisa kit
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >75 publications with KGF and Carcinoma
 Hyperplasia Antibodies  >65 publications with KGF and Hyperplasia
 Lung Injury Antibodies  >64 publications with KGF and Lung Injury
 Inflammation Antibodies  >50 publications with KGF and Inflammation
 Fibrosis Antibodies  >38 publications with KGF and Fibrosis
 Necrosis Antibodies  >35 publications with KGF and Necrosis
 Prostatic Hyperplasia Antibodies  >27 publications with KGF and Prostatic Hyperplasia
 Breast Neoplasms Antibodies  >25 publications with KGF and Breast Neoplasms
 Pulmonary Fibrosis Antibodies  >21 publications with KGF and Pulmonary Fibrosis
 Neoplasms, Experimental Antibodies  >20 publications with KGF and Neoplasms, Experimental
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >173 publications with KGF and Lung
 Skin Antibodies  >163 publications with KGF and Skin
 Embryonic Tissue Antibodies  >115 publications with KGF and Embryonic Tissue
 Prostate Antibodies  >74 publications with KGF and Prostate
 Bone Antibodies  >74 publications with KGF and Bone
 Intestine Antibodies  >70 publications with KGF and Intestine
 Vascular Antibodies  >53 publications with KGF and Vascular
 Kidney Antibodies  >44 publications with KGF and Kidney
 Liver Antibodies  >37 publications with KGF and Liver
 Muscle Antibodies  >37 publications with KGF and Muscle
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