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KL elisa kit :: Human Klotho ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2886510
Typical Testing Data/Standard Curve
Unit / Price
48-Strip-Wells  /  $380 +1 FREE 8GB USB
96-Strip-Wells  /  $500 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,170 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,840 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Klotho, ELISA Kit

★Popular Item★
 Also Known As   

Human Klotho ELISA Kit

 Product Synonym Names    Klotho; KL; 3.2.1.31
 Product Gene Name   

KL elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2886510 Testing Data    Testing Data PDF
 OMIM    211900
 3D Structure    ModBase 3D Structure for Q9UEF7
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 Species Reactivity    Human
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 Assay Type    Sandwich
 Detection Range    7.8-500 pg/mL
 Sensitivity    2.4 pg/mL
 Intra-Assay CV    <=4.7%
 Inter-Assay CV    <=7.1%
 Recovery    104%
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 Preparation and Storage    For long term storage, please store the entire kit at -20 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of KL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for KL purchase    MBS2886510 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Klotho, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 7.8-500 pg/mL in biological research samples containing KL, with an estimated sensitivity of 2.4 pg/mL. The ELISA analytical biochemical technique of the MBS2886510 kit is based on KL antibody-KL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for KL elisa kit

   Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve of KL elisa kit    KL elisa kit Typical Testing Data/Standard Curve image
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Sample Manual Insert of MBS2886510. Click to request current manual
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NCBI/Uniprot data below describe general gene information for KL. It may not necessarily be applicable to this product.
 NCBI GI #    24497614
 NCBI GeneID    9365
 NCBI Accession #    NP_004786.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_004795.3 [Other Products]
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 UniProt Primary Accession #    Q9UEF7 [Other Products]
 UniProt Secondary Accession #    Q5VZ95; Q96KV5; Q96KW5; Q9UEI9; Q9Y4F0 [Other Products]
 UniProt Related Accession #    Q9UEF7 [Other Products]
 Molecular Weight    62,135 Da
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 NCBI Official Full Name    klotho
 NCBI Official Synonym Full Names    klotho
 NCBI Official Symbol    KL [Similar Products]
 NCBI Protein Information    klotho
 UniProt Protein Name    Klotho
 Protein Family    Klotho
 UniProt Gene Name    KL [Similar Products]
 UniProt Entry Name    KLOT_HUMAN
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 NCBI Summary for KL    This gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss. [provided by RefSeq, Jul 2008]
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 UniProt Comments for KL    Klotho: May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1. Defects in KL are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC). A severe metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Some patients manifest recurrent, transient, painful swellings of the long bones associated with the radiographic findings of periosteal reaction and cortical hyperostosis and absence of skin involvement. Belongs to the glycosyl hydrolase 1 family. Klotho subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.2.1.31; Membrane protein, integral; Hydrolase

Chromosomal Location of Human Ortholog: 13q12

Cellular Component: apical plasma membrane; extracellular region; extracellular space; integral to membrane; integral to plasma membrane; intracellular; plasma membrane

Molecular Function: beta-glucosidase activity; beta-glucuronidase activity; fibroblast growth factor binding; fibroblast growth factor receptor binding; hormone activity; signal transducer activity; vitamin D binding

Biological Process: activation of MAPKK activity; aging; axon guidance; calcium ion homeostasis; carbohydrate metabolic process; energy reserve metabolic process; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; innate immune response; insulin receptor signaling pathway; MAPKKK cascade; nerve growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of bone mineralization; Ras protein signal transduction; small GTPase mediated signal transduction; vascular endothelial growth factor receptor signaling pathway

Disease: Tumoral Calcinosis, Hyperphosphatemic, Familial
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 Research Articles on KL    1. We have shown that circulating s-klotho was not related to FGF-23 in chronic kidney disease, dialysis and kidney transplant recipients patients
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with KL elisa kitPathways associated with KL elisa kit
 Reference Product  PubMed Publications
 FGF23 elisa kit  >48 publications with KL and FGF23
 FGFR1 elisa kit  >4 publications with KL and FGFR1
 Products by Pathway  Pathway Diagram
 ARMS-mediated Activation Pathway antibodies  ARMS-mediated Activation Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Constitutive Signaling By Aberrant PI3K In Cancer Pathway antibodies  Constitutive Signaling By Aberrant PI3K In Cancer Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Diseases Of Signal Transduction Pathway antibodies  Diseases Of Signal Transduction Pathway Diagram
Diseases associated with KL elisa kitOrgans/Tissues associated with KL elisa kit
 Disease Name  Pubmed Publications
 Lung Diseases Antibodies  >473 publications with KL and Lung Diseases
 Fibrosis Antibodies  >163 publications with KL and Fibrosis
 Skin Diseases Antibodies  >140 publications with KL and Skin Diseases
 Inflammation Antibodies  >105 publications with KL and Inflammation
 Death Antibodies  >91 publications with KL and Death
 Heart Diseases Antibodies  >80 publications with KL and Heart Diseases
 Lung Injury Antibodies  >67 publications with KL and Lung Injury
 Kidney Diseases Antibodies  >65 publications with KL and Kidney Diseases
 Necrosis Antibodies  >51 publications with KL and Necrosis
 Hypertension Antibodies  >50 publications with KL and Hypertension
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >326 publications with KL and Bone
 Brain Antibodies  >260 publications with KL and Brain
 Kidney Antibodies  >144 publications with KL and Kidney
 Connective Tissue Antibodies  >80 publications with KL and Connective Tissue
 Eye Antibodies  >41 publications with KL and Eye
 Spleen Antibodies  >38 publications with KL and Spleen
 Intestine Antibodies  >26 publications with KL and Intestine
 Parathyroid Antibodies  >18 publications with KL and Parathyroid
 Stomach Antibodies  >17 publications with KL and Stomach
 Prostate Antibodies  >17 publications with KL and Prostate
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