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KLF4 elisa kit :: Human Kruppel-like factor 4 (gut) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS928766
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Kruppel-like factor 4 (gut) (KLF4), ELISA Kit

★Popular Item★
 Also Known As   

Human Krueppel-like factor 4, KLF4 ELISA Kit

 Product Synonym Names    Human Krueppel-like factor 4 (KLF4) ELISA kit; EZF; GKLF; endothelial Kruppel-like zinc finger protein; Kruppel-like factor 4 (gut)
 Product Gene Name   

KLF4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS928766 Testing Data    Testing Data PDF
 OMIM    602253
 3D Structure    ModBase 3D Structure for O43474
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human KLF4. No significant cross-reactivity or interference between Human KLF4 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    18.75 pg/ml-1200 pg/ml
 Sensitivity    4.68 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Transcription/Transcription regulation
 Protein Biological Process 3    Transcription
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of KLF4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for KLF4 purchase    MBS928766 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Kruppel-like factor 4 (gut) (KLF4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 18.75 pg/ml-1200 pg/ml in biological research samples containing KLF4, with an estimated sensitivity of 4.68 pg/ml. The ELISA analytical biochemical technique of the MBS928766 kit is based on KLF4 antibody-KLF4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KLF4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KLF4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for KLF4 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KLF4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KLF4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLF4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLF4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of KLF4 elisa kit    KLF4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS928766. Click to request current manual
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NCBI/Uniprot data below describe general gene information for KLF4. It may not necessarily be applicable to this product.
 NCBI GI #    194248077
 NCBI GeneID    9314
 NCBI Accession #    NP_004226.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_004235.4 [Other Products]
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 UniProt Primary Accession #    O43474 [Other Products]
 UniProt Secondary Accession #    P78338; Q5T3J8; Q5T3J9; Q8N717; Q9UNP3; B2R8S4; B3KT79; L0R3I6; L0R4N5 [Other Products]
 UniProt Related Accession #    O43474 [Other Products]
 Molecular Weight    54,671 Da
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 NCBI Official Full Name    Krueppel-like factor 4
 NCBI Official Synonym Full Names    Kruppel-like factor 4 (gut)
 NCBI Official Symbol    KLF4 [Similar Products]
 NCBI Official Synonym Symbols   
EZF; GKLF
[Similar Products]
 NCBI Protein Information    Krueppel-like factor 4; gut-enriched krueppel-like factor; epithelial zinc finger protein EZF; endothelial Kruppel-like zinc finger protein
 UniProt Protein Name    Krueppel-like factor 4
 UniProt Synonym Protein Names   
Epithelial zinc finger protein EZF; Gut-enriched krueppel-like factor
 Protein Family    Krueppel-like factor
 UniProt Gene Name    KLF4 [Similar Products]
 UniProt Synonym Gene Names    EZF; GKLF [Similar Products]
 UniProt Entry Name    KLF4_HUMAN
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 UniProt Comments for KLF4    KLF4: Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription. Interacts with POU5F1/OCT4 and SOX2. Interacts with MUC1 (via the C-terminal domain). Belongs to the krueppel C2H2-type zinc-finger protein family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 9q31

Cellular Component: nucleoplasm; transcription factor complex; nuclear chromatin

Molecular Function: protein binding; zinc ion binding; double-stranded DNA binding; phosphoinositide 3-kinase regulator activity; transcription factor activity

Biological Process: fat cell differentiation; transcription from RNA polymerase II promoter; post-embryonic camera-type eye development; positive regulation of nitric oxide biosynthetic process; negative regulation of smooth muscle cell proliferation; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; positive regulation of cellular protein metabolic process; negative regulation of interleukin-8 biosynthetic process; negative regulation of muscle hyperplasia; negative regulation of transcription from RNA polymerase II promoter; negative regulation of caspase activity; positive regulation of protein metabolic process; positive regulation of hemoglobin biosynthetic process; regulation of phosphoinositide 3-kinase activity; negative regulation of cell proliferation; post-embryonic hemopoiesis; epidermis morphogenesis; regulation of cell differentiation; negative regulation of protein kinase B signaling cascade; response to retinoic acid; negative regulation of heterotypic cell-cell adhesion; stem cell maintenance; positive regulation of telomerase activity; inhibition of NF-kappaB transcription factor; negative regulation of inflammatory response; epidermal cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; mesodermal cell fate determination; negative regulation of phosphoinositide 3-kinase cascade
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 Research Articles on KLF4    1. KLF4 may play an anti-oncogenic role in gastric tumorigenesis.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with KLF4 elisa kitPathways associated with KLF4 elisa kit
 Reference Product  PubMed Publications
 SP1 elisa kit  >32 publications with KLF4 and SP1
 KLF6 elisa kit  >11 publications with KLF4 and KLF6
 TP53 elisa kit  >8 publications with KLF4 and TP53
 HDAC5 elisa kit  >3 publications with KLF4 and HDAC5
 ELK1 elisa kit  >2 publications with KLF4 and ELK1
 CREBBP elisa kit  >1 publications with KLF4 and CREBBP
 Products by Pathway  Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Diabetes Pathways antibodies  Diabetes Pathways Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway antibodies  Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway Diagram
 Synthesis, Secretion, And Deacylation Of Ghrelin Pathway antibodies  Synthesis, Secretion, And Deacylation Of Ghrelin Pathway Diagram
 Transcriptional Regulation Of White Adipocyte Differentiation Pathway antibodies  Transcriptional Regulation Of White Adipocyte Differentiation Pathway Diagram
Diseases associated with KLF4 elisa kitOrgans/Tissues associated with KLF4 elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >175 publications with KLF4 and Neoplasms
 Gastrointestinal Diseases Antibodies  >54 publications with KLF4 and Gastrointestinal Diseases
 Skin Diseases Antibodies  >39 publications with KLF4 and Skin Diseases
 Cardiovascular Diseases Antibodies  >32 publications with KLF4 and Cardiovascular Diseases
 Inflammation Antibodies  >31 publications with KLF4 and Inflammation
 Vascular Diseases Antibodies  >29 publications with KLF4 and Vascular Diseases
 Nervous System Diseases Antibodies  >27 publications with KLF4 and Nervous System Diseases
 Breast Neoplasms Antibodies  >20 publications with KLF4 and Breast Neoplasms
 Adenocarcinoma Antibodies  >18 publications with KLF4 and Adenocarcinoma
 Disease Models, Animal Antibodies  >18 publications with KLF4 and Disease Models, Animal
 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >118 publications with KLF4 and Embryonic Tissue
 Skin Antibodies  >79 publications with KLF4 and Skin
 Vascular Antibodies  >64 publications with KLF4 and Vascular
 Heart Antibodies  >40 publications with KLF4 and Heart
 Intestine Antibodies  >36 publications with KLF4 and Intestine
 Brain Antibodies  >32 publications with KLF4 and Brain
 Liver Antibodies  >27 publications with KLF4 and Liver
 Eye Antibodies  >22 publications with KLF4 and Eye
 Mouth Antibodies  >17 publications with KLF4 and Mouth
 Testis Antibodies  >17 publications with KLF4 and Testis
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