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LDHB elisa kit :: Human LDHB ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2500565 (SPECIAL PROMOTIONAL PRICING for a limited time)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $310 +1 FREE 8GB USB
96-Strip-Wells  /  $355 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,435 +1 FREE 8GB USB
10x96-Strip-Wells  /  $2,805 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

LDHB, ELISA Kit

★Popular Item★
 Also Known As   

Human LDHB (Lactate Dehydrogenase B) ELISA Kit

 Product Gene Name   

LDHB elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2500565 COA    COA PDF
 OMIM    150100
 3D Structure    ModBase 3D Structure for P07195
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 Species Reactivity    Human
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 Specificity    This kit recognizes natural and recombinant Human LDHB. No significant cross-reactivity or interference between Human LDHB and analogues was observed.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    3.125-200ng/mL
 Sensitivity    Min: 1.875ng/mL; Max: 200ng/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of LDHB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for LDHB purchase    MBS2500565 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the LDHB, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 3.125-200ng/mL in biological research samples containing LDHB, with an estimated sensitivity of Min: 1.875ng/mL; Max: 200ng/mL. The ELISA analytical biochemical technique of the MBS2500565 kit is based on LDHB antibody-LDHB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LDHB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LDHB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for LDHB elisa kit

   Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human LDHB concentrations in serum, plasma and other biological fluids.

Principle of the Assay||This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to LDHB. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for LDHB and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain LDHB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of LDHB. You can calculate the concentration of LDHB in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of LDHB elisa kit    LDHB elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2500565. Click to request current manual
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NCBI/Uniprot data below describe general gene information for LDHB. It may not necessarily be applicable to this product.
 NCBI GI #    4557032
 NCBI GeneID    3945
 NCBI Accession #    NP_002291.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_002300.6 [Other Products]
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 UniProt Primary Accession #    P07195 [Other Products]
 UniProt Related Accession #    P07195 [Other Products]
 Molecular Weight    36,638 Da
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 NCBI Official Full Name    L-lactate dehydrogenase B chain
 NCBI Official Synonym Full Names    lactate dehydrogenase B
 NCBI Official Symbol    LDHB [Similar Products]
 NCBI Official Synonym Symbols   
LDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281
[Similar Products]
 NCBI Protein Information    L-lactate dehydrogenase B chain; LDH heart subunit; epididymis secretory protein Li 281; lactate dehydrogenase H chain; renal carcinoma antigen NY-REN-46
 UniProt Protein Name    L-lactate dehydrogenase B chain
 UniProt Synonym Protein Names   
LDH heart subunit; LDH-H; Renal carcinoma antigen NY-REN-46
 Protein Family    L-lactate dehydrogenase
 UniProt Gene Name    LDHB [Similar Products]
 UniProt Synonym Gene Names    LDH-B; LDH-H [Similar Products]
 UniProt Entry Name    LDHB_HUMAN
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 NCBI Summary for LDHB    This gene encodes an enzyme which catalyzes the reversible conversion of lactate and pyruvate, and NAD and NADH, in the glycolytic pathway. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on the X chromosome and on chromosome. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Mar 2010]
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 Research Articles on LDHB    1. We were able to provide evidence that methylation of HLTF and especially HPP1 detected in serum is strongly correlated with cell death in CRC using LDH as surrogate marker
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with LDHB elisa kitPathways associated with LDHB elisa kit
 Reference Product  PubMed Publications
 GPI elisa kit  >11 publications with LDHB and GPI
 PKM2 elisa kit  >7 publications with LDHB and PKM2
 ME1 elisa kit  >6 publications with LDHB and ME1
 GOT1 elisa kit  >3 publications with LDHB and GOT1
 PDHB elisa kit  >1 publications with LDHB and PDHB
 PKLR elisa kit  >1 publications with LDHB and PKLR
 Products by Pathway  Pathway Diagram
 Abnormal Metabolism In Phenylketonuria Pathway antibodies  Abnormal Metabolism In Phenylketonuria Pathway Diagram
 Cysteine And Methionine Metabolism Pathway antibodies  Cysteine And Methionine Metabolism Pathway Diagram
 Cysteine And Methionine Metabolism Pathway antibodies  Cysteine And Methionine Metabolism Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Glycolysis / Gluconeogenesis Pathway antibodies  Glycolysis / Gluconeogenesis Pathway Diagram
 Glycolysis / Gluconeogenesis Pathway antibodies  Glycolysis / Gluconeogenesis Pathway Diagram
 Glycolysis And Gluconeogenesis Pathway antibodies  Glycolysis And Gluconeogenesis Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Propanoate Metabolism Pathway antibodies  Propanoate Metabolism Pathway Diagram
Diseases associated with LDHB elisa kitOrgans/Tissues associated with LDHB elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >6 publications with LDHB and Nervous System Diseases
 Breast Neoplasms Antibodies  >6 publications with LDHB and Breast Neoplasms
 Neoplasms, Experimental Antibodies  >4 publications with LDHB and Neoplasms, Experimental
 Carcinoma, Hepatocellular Antibodies  >4 publications with LDHB and Carcinoma, Hepatocellular
 Cardiovascular Diseases Antibodies  >4 publications with LDHB and Cardiovascular Diseases
 Liver Neoplasms Antibodies  >3 publications with LDHB and Liver Neoplasms
 Heart Diseases Antibodies  >3 publications with LDHB and Heart Diseases
 Lung Neoplasms Antibodies  >3 publications with LDHB and Lung Neoplasms
 Liver Diseases Antibodies  >3 publications with LDHB and Liver Diseases
 Inflammation Antibodies  >2 publications with LDHB and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >29 publications with LDHB and Blood
 Muscle Antibodies  >13 publications with LDHB and Muscle
 Testis Antibodies  >10 publications with LDHB and Testis
 Heart Antibodies  >10 publications with LDHB and Heart
 Brain Antibodies  >9 publications with LDHB and Brain
 Liver Antibodies  >5 publications with LDHB and Liver
 Lung Antibodies  >4 publications with LDHB and Lung
 Lymph Node Antibodies  >4 publications with LDHB and Lymph Node
 Thyroid Antibodies  >4 publications with LDHB and Thyroid
 Bone Antibodies  >4 publications with LDHB and Bone
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