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LFA-3/CD58 elisa kit :: Human LFA-3 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2512588
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

LFA-3 (LFA-3/CD58), ELISA Kit

★Popular Item★
 Also Known As   

Human LFA-3 (Lymphocyte Function Associated Antigen 3) ELISA Kit

 Product Gene Name   

LFA-3/CD58 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    153420
 3D Structure    ModBase 3D Structure for P19256
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human LFA-3/CD58. No significant cross-reactivity or interference between Human LFA-3/CD58 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human LFA-3/CD58 and all the analogues, therefore, cross reaction may still exist.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    12.5-800pg/mL
 Sensitivity    Min: 7.5pg/mL; Max: 800pg/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of LFA-3/CD58 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for LFA-3/CD58 purchase    MBS2512588 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the LFA-3 (LFA-3/CD58) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 12.5-800pg/mL in biological research samples containing LFA-3/CD58, with an estimated sensitivity of Min: 7.5pg/mL; Max: 800pg/mL. The ELISA analytical biochemical technique of the MBS2512588 kit is based on LFA-3/CD58 antibody-LFA-3/CD58 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LFA-3/CD58 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LFA-3/CD58. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for LFA-3/CD58 elisa kit

   Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of LFA-3/CD58 in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LFA-3/CD58. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human LFA-3/CD58 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LFA-3/CD58, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human LFA-3/CD58. You can calculate the concentration of Human LFA-3/CD58 in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of LFA-3/CD58 elisa kit    LFA-3/CD58 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2512588. Click to request current manual
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NCBI/Uniprot data below describe general gene information for LFA-3/CD58. It may not necessarily be applicable to this product.
 NCBI GI #    221316575
 NCBI GeneID    965
 NCBI Accession #    NP_001138294.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001144822.1 [Other Products]
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 UniProt Primary Accession #    P19256 [Other Products]
 UniProt Secondary Accession #    Q5U053; Q6IB65; Q96KI9; A8K7G5 [Other Products]
 UniProt Related Accession #    P19256 [Other Products]
 Molecular Weight    27,960 Da
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 NCBI Official Full Name    lymphocyte function-associated antigen 3 isoform 2
 NCBI Official Synonym Full Names    CD58 molecule
 NCBI Official Symbol    CD58 [Similar Products]
 NCBI Official Synonym Symbols   
ag3; LFA3; LFA-3
[Similar Products]
 NCBI Protein Information    lymphocyte function-associated antigen 3; CD58 antigen, (lymphocyte function-associated antigen 3); surface glycoprotein LFA-3
 UniProt Protein Name    Lymphocyte function-associated antigen 3
 UniProt Synonym Protein Names   
Surface glycoprotein LFA-3; CD_antigen: CD58
 UniProt Gene Name    CD58 [Similar Products]
 UniProt Synonym Gene Names    LFA3; Ag3 [Similar Products]
 UniProt Entry Name    LFA3_HUMAN
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 NCBI Summary for LFA-3/CD58    This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a ligand of the T lymphocyte CD2 protein, and functions in adhesion and activation of T lymphocytes. The protein is localized to the plasma membrane. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2009]
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 UniProt Comments for LFA-3/CD58    CD58: Ligand of the T-lymphocyte CD2 glycoprotein. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and -dependent interactions of T-lymphocytes with target cells and antigen- presenting cells and the T-lymphocyte rosetting with erythrocytes. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 1p13

Cellular Component: cell surface; membrane; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; receptor binding

Biological Process: heterotypic cell-cell adhesion; cell-cell adhesion; blood coagulation; cell adhesion; leukocyte migration
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 Research Articles on LFA-3/CD58    1. Genetic variations in CD58 were associated with the susceptibility of neuromyelitis optica in a Korean population.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with LFA-3/CD58 elisa kitPathways associated with LFA-3/CD58 elisa kit
 Reference Product  PubMed Publications
 CD2 elisa kit  >416 publications with LFA-3/CD58 and CD2
 ICAM1 elisa kit  >289 publications with LFA-3/CD58 and ICAM1
 ITGAL elisa kit  >138 publications with LFA-3/CD58 and ITGAL
 CD86 elisa kit  >110 publications with LFA-3/CD58 and CD86
 CD40 elisa kit  >103 publications with LFA-3/CD58 and CD40
 IFNA17 elisa kit  >8 publications with LFA-3/CD58 and IFNA17
 CD2AP elisa kit  >1 publications with LFA-3/CD58 and CD2AP
 Products by Pathway  Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
Diseases associated with LFA-3/CD58 elisa kitOrgans/Tissues associated with LFA-3/CD58 elisa kit
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >110 publications with LFA-3/CD58 and Necrosis
 Leukemia Antibodies  >104 publications with LFA-3/CD58 and Leukemia
 Inflammation Antibodies  >43 publications with LFA-3/CD58 and Inflammation
 Nervous System Diseases Antibodies  >42 publications with LFA-3/CD58 and Nervous System Diseases
 Neoplasms, Experimental Antibodies  >40 publications with LFA-3/CD58 and Neoplasms, Experimental
 Cardiovascular Diseases Antibodies  >27 publications with LFA-3/CD58 and Cardiovascular Diseases
 Anemia Antibodies  >23 publications with LFA-3/CD58 and Anemia
 Liver Diseases Antibodies  >23 publications with LFA-3/CD58 and Liver Diseases
 Multiple Sclerosis Antibodies  >23 publications with LFA-3/CD58 and Multiple Sclerosis
 Cell Transformation, Neoplastic Antibodies  >22 publications with LFA-3/CD58 and Cell Transformation, Neoplastic
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >747 publications with LFA-3/CD58 and Blood
 Bone Antibodies  >86 publications with LFA-3/CD58 and Bone
 Bone Marrow Antibodies  >82 publications with LFA-3/CD58 and Bone Marrow
 Skin Antibodies  >41 publications with LFA-3/CD58 and Skin
 Lung Antibodies  >33 publications with LFA-3/CD58 and Lung
 Liver Antibodies  >31 publications with LFA-3/CD58 and Liver
 Brain Antibodies  >24 publications with LFA-3/CD58 and Brain
 Embryonic Tissue Antibodies  >20 publications with LFA-3/CD58 and Embryonic Tissue
 Lymph Node Antibodies  >18 publications with LFA-3/CD58 and Lymph Node
 Kidney Antibodies  >18 publications with LFA-3/CD58 and Kidney
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