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LMX1B elisa kit :: Human LIM homeobox transcription factor 1-beta ELISA Kit

Scan QR to view Datasheet Catalog #    MBS760099
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $300 +1 FREE 8GB USB
96-Strip-Wells  /  $415 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

LIM homeobox transcription factor 1-beta (LMX1B), ELISA Kit

★Popular Item★
 Also Known As   

Human LIM homeobox transcription factor 1-beta ELISA Kit

 Product Synonym Names    LMX1B (LIM homeobox transcription factor 1-beta)/LMX-1.2/LIM,homeobox protein 1.2/LIM,homeobox protein LMX1B
 Product Gene Name   

LMX1B elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS760099 COA    COA PDF
 OMIM    161200
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of LMX1B. No significant cross-reactivity or interference between LMX1B and analogues was observed.
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 Samples    Serum, plasma and other biological fluids.
 Assay Type    Competitive ELISA, Coated with Antigen
 Detection Range    0.312-20ng/ml
 Sensitivity    0.188ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LMX1B were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LMX1B were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of LMX1B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for LMX1B purchase    MBS760099 is a ready-to-use microwell, strip plate Competitive, Coated with Antigen ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the LIM homeobox transcription factor 1-beta (LMX1B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312-20ng/ml in biological research samples containing LMX1B, with an estimated sensitivity of 0.188ng/ml. The ELISA analytical biochemical technique of the MBS760099 kit is based on LMX1B antibody-LMX1B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LMX1B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LMX1B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for LMX1B elisa kit

   Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with LMX1B. During the reaction, LMX1B in the sample or standard competes with a fixed amount of LMX1B on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to LMX1B. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of LMX1B in the samples is then determined by comparing the OD. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of LMX1B elisa kit    LMX1B elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS760099. Click to request current manual
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NCBI/Uniprot data below describe general gene information for LMX1B. It may not necessarily be applicable to this product.
 NCBI GI #    292494915
 NCBI GeneID    4010
 NCBI Accession #    NP_001167618.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001174147.1 [Other Products]
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 UniProt Secondary Accession #    O75463; Q5JU95; Q6ISC9; F8W7W6 [Other Products]
 UniProt Related Accession #    O60663 [Other Products]
 Molecular Weight    45,080 Da
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 NCBI Official Full Name    LIM homeobox transcription factor 1-beta isoform 2
 NCBI Official Synonym Full Names    LIM homeobox transcription factor 1 beta
 NCBI Official Symbol    LMX1B [Similar Products]
 NCBI Official Synonym Symbols   
NPS1; LMX1.2
[Similar Products]
 NCBI Protein Information    LIM homeobox transcription factor 1-beta
 UniProt Protein Name    LIM homeobox transcription factor 1-beta
 UniProt Synonym Protein Names   
LIM/homeobox protein 1.2; LMX-1.2; LIM/homeobox protein LMX1B
 Protein Family    LIM/homeobox protein
 UniProt Gene Name    LMX1B [Similar Products]
 UniProt Synonym Gene Names    LMX-1.2 [Similar Products]
 UniProt Entry Name    LMX1B_HUMAN
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 NCBI Summary for LMX1B    This gene encodes a member of LIM-homeodomain family of proteins containing two N-terminal zinc-binding LIM domains, 1 homeodomain, and a C-terminal glutamine-rich domain. It functions as a transcription factor, and is essential for the normal development of dorsal limb structures, the glomerular basement membrane, the anterior segment of the eye, and dopaminergic and serotonergic neurons. Mutations in this gene are associated with nail-patella syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]
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 UniProt Comments for LMX1B    LMX1B: Essential for the specification of dorsal limb fate at both the zeugopodal and autopodal levels. Defects in LMX1B are the cause of nail-patella syndrome (NPS); also known as onychoosteodysplasia. NPS is a disease that cause abnormal skeletal patterning and renal dysplasia. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 9q33.3

Cellular Component: nucleus

Molecular Function: protein binding; transcription factor activity; zinc ion binding

Biological Process: dorsal/ventral pattern formation; in utero embryonic development; multicellular organismal development; neuron differentiation; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; transcription, DNA-dependent

Disease: Nail-patella Syndrome
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 Research Articles on LMX1B    1. these results reveal a sustained and essential requirement of Lmx1b for the function of midbrain dopamine neurons
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with LMX1B elisa kitPathways associated with LMX1B elisa kit
 Reference Product  PubMed Publications
 WNT1 elisa kit  >18 publications with LMX1B and WNT1
 FGF8 elisa kit  >16 publications with LMX1B and FGF8
 NR4A2 elisa kit  >12 publications with LMX1B and NR4A2
 WNT7A elisa kit  >10 publications with LMX1B and WNT7A
 OTX2 elisa kit  >10 publications with LMX1B and OTX2
 LDB1 elisa kit  >8 publications with LMX1B and LDB1
 FEV elisa kit  >7 publications with LMX1B and FEV
 GBX2 elisa kit  >3 publications with LMX1B and GBX2
 ATOH1 elisa kit  >2 publications with LMX1B and ATOH1
 Products by Pathway  Pathway Diagram
 SIDS Susceptibility Pathways antibodies  SIDS Susceptibility Pathways Diagram
Diseases associated with LMX1B elisa kitOrgans/Tissues associated with LMX1B elisa kit
 Disease Name  Pubmed Publications
 Nail-Patella Syndrome Antibodies  >83 publications with LMX1B and Nail-Patella Syndrome
 Kidney Diseases Antibodies  >32 publications with LMX1B and Kidney Diseases
 Brain Diseases Antibodies  >16 publications with LMX1B and Brain Diseases
 Proteinuria Antibodies  >15 publications with LMX1B and Proteinuria
 Pain Antibodies  >13 publications with LMX1B and Pain
 Mental Disorders Antibodies  >9 publications with LMX1B and Mental Disorders
 Glaucoma 1, Open Angle, A Antibodies  >8 publications with LMX1B and Glaucoma 1, Open Angle, A
 Renal Insufficiency Antibodies  >7 publications with LMX1B and Renal Insufficiency
 Hematuria Antibodies  >5 publications with LMX1B and Hematuria
 Neurodevelopmental Disorders Antibodies  >4 publications with LMX1B and Neurodevelopmental Disorders
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >94 publications with LMX1B and Brain
 Eye Antibodies  >24 publications with LMX1B and Eye
 Mixed Antibodies  >2 publications with LMX1B and Mixed
 Testis Antibodies  >1 publications with LMX1B and Testis
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