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LMNB1 elisa kit :: Human Lamin B1 (LMNB1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2024959 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Standard Curve/Testing Data
Unit / Price
24-Strip-Wells  /  $270 +1 FREE 8GB USB
48-Strip-Wells  /  $505 +1 FREE 8GB USB
96-Strip-Wells  /  $685 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,770 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,025 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Lamin B1 (LMNB1), ELISA Kit

★Popular Item★
 Also Known As   

Lamin B1 (LMNB1) ELISA Kit

 Product Synonym Names    LMN; LMN2; LMNB
 Product Gene Name   

LMNB1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    150340
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Lamin B1 (LMNB1).
No significant cross-reactivity or interference between Lamin B1 (LMNB1) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Tissue homogenates, Cell lysates and Other Biological Fluids
 Detection Range    0.156-10ng/mL
 Sensitivity    < 0.057ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Lamin B1 (LMNB1) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Lamin B1 (LMNB1) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of LMNB1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for LMNB1 purchase    MBS2024959 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Lamin B1 (LMNB1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing LMNB1, with an estimated sensitivity of < 0.057ng/mL. The ELISA analytical biochemical technique of the MBS2024959 kit is based on LMNB1 antibody-LMNB1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LMNB1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LMNB1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates, Cell lysates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for LMNB1 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lamin B1 (LMNB1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lamin B1 (LMNB1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lamin B1 (LMNB1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lamin B1 (LMNB1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for LMNB1 elisa kit    Metabolic pathway
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 Typical Standard Curve/Testing Data of LMNB1 elisa kit    LMNB1 elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS2024959. Click to request current manual
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NCBI/Uniprot data below describe general gene information for LMNB1. It may not necessarily be applicable to this product.
 NCBI GI #    576840
 NCBI GeneID    4001
 NCBI Accession #    AAC37575.1 [Other Products]
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 UniProt Secondary Accession #    Q3SYN7; Q96EI6; B2R6J6 [Other Products]
 UniProt Related Accession #    P20700 [Other Products]
 Molecular Weight    66,408 Da
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 NCBI Official Full Name    lamin B1
 NCBI Official Synonym Full Names    lamin B1
 NCBI Official Symbol    LMNB1 [Similar Products]
 NCBI Official Synonym Symbols   
LMN; ADLD; LMN2; LMNB
[Similar Products]
 NCBI Protein Information    lamin-B1
 UniProt Protein Name    Lamin-B1
 Protein Family    Lamin
 UniProt Gene Name    LMNB1 [Similar Products]
 UniProt Synonym Gene Names    LMN2; LMNB [Similar Products]
 UniProt Entry Name    LMNB1_HUMAN
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 NCBI Summary for LMNB1    The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Alternative splicing results in transcript variants and a duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). [provided by RefSeq, Oct 2010]
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 UniProt Comments for LMNB1    Lamin B1: nuclear lamins are intermediate filament proteins that constitute the lattice-like matrix at the inner face of the nuclear membrane that underlies the nuclear envelop. The lamins, highly conserved throughout evolution, are encoded by three genes in the human: LMNA, LMNB1, and LMNB2. The A-type lamins (lamin A/C) are developmentally regulated and are generally expressed in differentiated cells. The anchoring of chromatin to the nuclear lamina is involved in the control of gene expression and in DNA replication and repair. During mitosis, the nuclear lamina is reversibly disassembled as the lamin proteins are phosphorylated. B-type lamins are phosphorylated within minutes of engaging the IgM surface receptor of resting splenic B cells. Nuclear lamins are cleaved by caspases during apoptosis.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 5q23.2

Cellular Component: nucleoplasm; nuclear membrane; membrane; lamin filament; nuclear inner membrane; nuclear envelope

Molecular Function: phospholipase binding; structural molecule activity

Biological Process: apoptosis; cell structure disassembly during apoptosis

Disease: Leukodystrophy, Demyelinating, Adult-onset, Autosomal Dominant
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 Research Articles on LMNB1    1. maintenance of lamin B1 levels is required for DNA replication and repair through regulation of the expression of key factors involved in these essential nuclear functions
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with LMNB1 elisa kitPathways associated with LMNB1 elisa kit
 Reference Product  PubMed Publications
 LMNA elisa kit  >16 publications with LMNB1 and LMNA
 LMNB2 elisa kit  >13 publications with LMNB1 and LMNB2
 BRCA1 elisa kit  >1 publications with LMNB1 and BRCA1
 CDK1 elisa kit  >1 publications with LMNB1 and CDK1
 Products by Pathway  Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptotic Cleavage Of Cellular Proteins Pathway antibodies  Apoptotic Cleavage Of Cellular Proteins Pathway Diagram
 Apoptotic Execution Phase Pathway antibodies  Apoptotic Execution Phase Pathway Diagram
 Breakdown Of The Nuclear Lamina Pathway antibodies  Breakdown Of The Nuclear Lamina Pathway Diagram
 Caspase Cascade In Apoptosis Pathway antibodies  Caspase Cascade In Apoptosis Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cellular Senescence Pathway antibodies  Cellular Senescence Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 DNA Damage/Telomere Stress Induced Senescence Pathway antibodies  DNA Damage/Telomere Stress Induced Senescence Pathway Diagram
Diseases associated with LMNB1 elisa kitOrgans/Tissues associated with LMNB1 elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >21 publications with LMNB1 and Nervous System Diseases
 Carcinoma Antibodies  >6 publications with LMNB1 and Carcinoma
 Adenocarcinoma Antibodies  >5 publications with LMNB1 and Adenocarcinoma
 Liver Diseases Antibodies  >4 publications with LMNB1 and Liver Diseases
 Pelizaeus-Merzbacher Disease Antibodies  >4 publications with LMNB1 and Pelizaeus-Merzbacher Disease
 Carcinoma, Hepatocellular Antibodies  >3 publications with LMNB1 and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >3 publications with LMNB1 and Liver Neoplasms
 Disease Models, Animal Antibodies  >2 publications with LMNB1 and Disease Models, Animal
 Pancreatic Neoplasms Antibodies  >1 publications with LMNB1 and Pancreatic Neoplasms
 Kidney Diseases Antibodies  >1 publications with LMNB1 and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >14 publications with LMNB1 and Brain
 Blood Antibodies  >9 publications with LMNB1 and Blood
 Skin Antibodies  >6 publications with LMNB1 and Skin
 Muscle Antibodies  >5 publications with LMNB1 and Muscle
 Liver Antibodies  >4 publications with LMNB1 and Liver
 Bone Antibodies  >4 publications with LMNB1 and Bone
 Kidney Antibodies  >1 publications with LMNB1 and Kidney
 Lung Antibodies  >1 publications with LMNB1 and Lung
 Intestine Antibodies  >1 publications with LMNB1 and Intestine
 Connective Tissue Antibodies  >1 publications with LMNB1 and Connective Tissue
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