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LAT1 elisa kit :: Human Large Neutral Amino Acid Transporter 1 (LAT1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2022184 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $270 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $665 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,715 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,895 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Large Neutral Amino Acid Transporter 1 (LAT1), ELISA Kit

★Popular Item★
 Also Known As   

Large Neutral Amino Acid Transporter 1 (LAT1) ELISA Kit

 Product Synonym Names    CD98; MDU1; 4T2HC; 4F2; NACAE; SLC7A5; MPE16; Solute Carrier Family 7 Member 5; Cationic Amino Acid Transporter,y+ System; L-Type Amino Acid Transporter
 Product Gene Name   

LAT1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    600182
 3D Structure    ModBase 3D Structure for Q01650
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of LAT1. No significant cross-reactivity or interference between LAT1 and analogues was observed.
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 Samples    Tissue homogenates, cell lysates and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    0.156-10ng/mL
 Sensitivity    0.058ng/mL
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LAT1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LAT1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of LAT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for LAT1 purchase    MBS2022184 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Large Neutral Amino Acid Transporter 1 (LAT1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing LAT1, with an estimated sensitivity of 0.058ng/mL. The ELISA analytical biochemical technique of the MBS2022184 kit is based on LAT1 antibody-LAT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LAT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LAT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates, cell lysates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for LAT1 elisa kit

   Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of LAT1 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to LAT1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to LAT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LAT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of LAT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for LAT1 elisa kit    Signal transduction; Metabolic pathway; Infection immunity
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 Typical Testing Data/Standard Curve (for reference only) of LAT1 elisa kit    LAT1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2022184. Click to request current manual
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NCBI/Uniprot data below describe general gene information for LAT1. It may not necessarily be applicable to this product.
 NCBI GI #    71979932
 NCBI GeneID    8140
 NCBI Accession #    NP_003477.4 [Other Products]
 NCBI GenBank Nucleotide #    NM_003486.5 [Other Products]
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 UniProt Primary Accession #    Q01650 [Other Products]
 UniProt Secondary Accession #    Q8IV97; Q9UBN8; Q9UP15; Q9UQC0 [Other Products]
 UniProt Related Accession #    Q01650 [Other Products]
 Molecular Weight    55,010 Da
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 NCBI Official Full Name    large neutral amino acids transporter small subunit 1
 NCBI Official Synonym Full Names    solute carrier family 7 (amino acid transporter light chain, L system), member 5
 NCBI Official Symbol    SLC7A5 [Similar Products]
 NCBI Official Synonym Symbols   
E16; CD98; LAT1; 4F2LC; MPE16; hLAT1; D16S469E
[Similar Products]
 NCBI Protein Information    large neutral amino acids transporter small subunit 1
 UniProt Protein Name    Large neutral amino acids transporter small subunit 1
 UniProt Synonym Protein Names   
4F2 light chain; 4F2 LC; 4F2LC; CD98 light chain; Integral membrane protein E16; L-type amino acid transporter 1; hLAT1; Solute carrier family 7 member 5; y+ system cationic amino acid transporter
 UniProt Gene Name    SLC7A5 [Similar Products]
 UniProt Synonym Gene Names    CD98LC; LAT1; MPE16; 4F2 LC; 4F2LC; hLAT1 [Similar Products]
 UniProt Entry Name    LAT1_HUMAN
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 UniProt Comments for LAT1    SLC7A5: Sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Involved in cellular amino acid uptake. Acts as an amino acid exchanger. Involved in the transport of L-DOPA across the blood- brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Plays a role in neuronal cell proliferation (neurogenesis) in brain. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L- nitrosocysteine (L-CNSO) across the transmembrane. May play an important role in high-grade gliomas. Mediates blood-to-retina L- leucine transport across the inner blood-retinal barrier which in turn may play a key role in maintaining large neutral amino acids as well as neurotransmitters in the neural retina. Acts as the major transporter of tyrosine in fibroblasts. Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc. Expression induced in quiescent peripheral blood lymphocytes after treatment with phorbol myristate acetate (PMA) and phytohemagglutinin (PHA). Expression and the uptake of leucine is stimulated in mononuclear, cytotrophoblast-like choriocarcinoma cells by combined treatment with PMA and calcium ionophore. Expressed abundantly in adult lung, liver, brain, skeletal muscle, placenta, bone marrow, testis, resting lymphocytes and monocytes, and in fetal liver. Weaker expression in thymus, cornea, retina, peripheral leukocytes, spleen, kidney, colon and lymph node. During gestation, expression in the placenta was significantly stronger at full-term than at the mid-trimester stage. Also expressed in all human tumor cell lines tested and in the astrocytic process of primary astrocytic gliomas. Expressed in retinal endothelial cells and in the intestinal epithelial cell line Caco-2. Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter, SLC family; Transporter

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: intracellular membrane-bound organelle; membrane; cytoplasm; apical plasma membrane; integral to membrane; plasma membrane; nucleolus; cytosol; nucleus

Molecular Function: L-amino acid transmembrane transporter activity; peptide antigen binding; neutral amino acid transmembrane transporter activity; amino acid transmembrane transporter activity

Biological Process: neutral amino acid transport; amino acid metabolic process; nervous system development; amino acid transport; transport; ion transport; blood coagulation; cell differentiation; transmembrane transport; leukocyte migration
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 Research Articles on LAT1    1. CAP-D3 down-regulates transcription of genes that encode amino acid transporters (SLC7A5 and SLC3A2) to promote bacterial autophagy by colon epithelial cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with LAT1 elisa kitPathways associated with LAT1 elisa kit
 Reference Product  PubMed Publications
 SLC3A2 elisa kit  >15 publications with LAT1 and SLC3A2
 SLC1A5 elisa kit  >14 publications with LAT1 and SLC1A5
 SLC3A1 elisa kit  >1 publications with LAT1 and SLC3A1
 Products by Pathway  Pathway Diagram
 Amino Acid Transport Across The Plasma Membrane Pathway antibodies  Amino Acid Transport Across The Plasma Membrane Pathway Diagram
 Basigin Interactions Pathway antibodies  Basigin Interactions Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 SLC-mediated Transmembrane Transport Pathway antibodies  SLC-mediated Transmembrane Transport Pathway Diagram
 Transmembrane Transport Of Small Molecules Pathway antibodies  Transmembrane Transport Of Small Molecules Pathway Diagram
 Transport Of Inorganic Cations/anions And Amino Acids/oligopeptides Pathway antibodies  Transport Of Inorganic Cations/anions And Amino Acids/oligopeptides Pathway Diagram
Diseases associated with LAT1 elisa kitOrgans/Tissues associated with LAT1 elisa kit
 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >27 publications with LAT1 and Adenocarcinoma
 Lung Neoplasms Antibodies  >22 publications with LAT1 and Lung Neoplasms
 Nervous System Diseases Antibodies  >21 publications with LAT1 and Nervous System Diseases
 Brain Diseases Antibodies  >20 publications with LAT1 and Brain Diseases
 Lung Diseases Antibodies  >20 publications with LAT1 and Lung Diseases
 Breast Neoplasms Antibodies  >14 publications with LAT1 and Breast Neoplasms
 Neoplasms, Experimental Antibodies  >10 publications with LAT1 and Neoplasms, Experimental
 Liver Neoplasms Antibodies  >8 publications with LAT1 and Liver Neoplasms
 Ovarian Neoplasms Antibodies  >7 publications with LAT1 and Ovarian Neoplasms
 Liver Diseases Antibodies  >6 publications with LAT1 and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >67 publications with LAT1 and Blood
 Brain Antibodies  >54 publications with LAT1 and Brain
 Lung Antibodies  >26 publications with LAT1 and Lung
 Kidney Antibodies  >26 publications with LAT1 and Kidney
 Liver Antibodies  >17 publications with LAT1 and Liver
 Muscle Antibodies  >16 publications with LAT1 and Muscle
 Eye Antibodies  >12 publications with LAT1 and Eye
 Lymph Antibodies  >12 publications with LAT1 and Lymph
 Lymph Node Antibodies  >11 publications with LAT1 and Lymph Node
 Prostate Antibodies  >9 publications with LAT1 and Prostate
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