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ACADL elisa kit :: Human Long chain specific acyl CoA dehydrogenase, mitochondrial (ACADL) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7201517
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
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 Product Name   

Long chain specific acyl CoA dehydrogenase, mitochondrial (ACADL), ELISA Kit

★Popular Item★
 Also Known As   

Human Long chain specific acyl CoA dehydrogenase, mitochondrial (ACADL) ELISA Kit

 Product Gene Name   

ACADL elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    609576
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of ACADL. No significant cross-reactivity or interference between ACADL and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ACADL and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    5.0-100ng/mL
 Sensitivity    0.1ng/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ACADL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ACADL purchase    MBS7201517 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Long chain specific acyl CoA dehydrogenase, mitochondrial (ACADL) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 5.0-100ng/mL in biological research samples containing ACADL, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS7201517 kit is based on ACADL antibody-ACADL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ACADL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ACADL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ACADL elisa kit

   Intended Uses||This ACADL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ACADL. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: ACADL ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ACADL antibody and an ACADL-HRP conjugate. The assay sample and buffer are incubated together with ACADL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACADL concentration since ACADL from samples and ACADL-HRP conjugate compete for the anti-ACADL antibody binding site. Since the number of sites is limited, as more sites are occupied by ACADL from the sample, fewer sites are left to bind ACADL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACADL concentration in each sample is interpolated from this standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of ACADL elisa kit    ACADL elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS7201517. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ACADL. It may not necessarily be applicable to this product.
 NCBI GI #    4501857
 NCBI GeneID    33
 NCBI Accession #    NP_001599.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001608.3 [Other Products]
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 UniProt Secondary Accession #    Q8IUN8; B2R8T3 [Other Products]
 UniProt Related Accession #    P28330 [Other Products]
 Molecular Weight    47,656 Da
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 NCBI Official Full Name    long-chain specific acyl-CoA dehydrogenase, mitochondrial
 NCBI Official Synonym Full Names    acyl-CoA dehydrogenase, long chain
 NCBI Official Symbol    ACADL [Similar Products]
 NCBI Official Synonym Symbols   
LCAD; ACAD4
[Similar Products]
 NCBI Protein Information    long-chain specific acyl-CoA dehydrogenase, mitochondrial; acyl-Coenzyme A dehydrogenase, long chain
 UniProt Protein Name    Long-chain specific acyl-CoA dehydrogenase, mitochondrial
 Protein Family    Long-chain specific acyl-CoA dehydrogenase
 UniProt Gene Name    ACADL [Similar Products]
 UniProt Synonym Gene Names    LCAD [Similar Products]
 UniProt Entry Name    ACADL_HUMAN
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 NCBI Summary for ACADL    The protein encoded by this gene belongs to the acyl-CoA dehydrogenase family, which is a family of mitochondrial flavoenzymes involved in fatty acid and branched chain amino-acid metabolism. This protein is one of the four enzymes that catalyze the initial step of mitochondrial beta-oxidation of straight-chain fatty acid. Defects in this gene are the cause of long-chain acyl-CoA dehydrogenase (LCAD) deficiency, leading to nonketotic hypoglycemia. [provided by RefSeq, Jul 2008]
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 UniProt Comments for ACADL    ACADL: Defects in ACADL are a cause of acyl-CoA dehydrogenase very long-chain deficiency (ACADVLD). An inborn error of mitochondrial fatty acid beta-oxidation which leads to impaired long-chain fatty acid beta-oxidation. It is clinically heterogeneous, with three major phenotypes: a severe childhood form characterized by early onset, high mortality and high incidence of cardiomyopathy; a milder childhood form with later onset, characterized by hypoketotic hypoglycemia, low mortality and rare cardiomyopathy; an adult form, with isolated skeletal muscle involvement, rhabdomyolysis and myoglobinuria, usually triggered by exercise or fasting. Belongs to the acyl-CoA dehydrogenase family.

Protein type: Lipid Metabolism - fatty acid; EC 1.3.8.8; Mitochondrial; Oxidoreductase

Chromosomal Location of Human Ortholog: 2q34

Cellular Component: mitochondrion; mitochondrial matrix; cytoplasm; mitochondrial membrane

Molecular Function: palmitoyl-CoA oxidase activity; acyl-CoA dehydrogenase activity; FAD binding; acyl-CoA binding; long-chain-acyl-CoA dehydrogenase activity

Biological Process: carnitine metabolic process, CoA-linked; fatty acid beta-oxidation; cellular lipid catabolic process; carnitine catabolic process; thermoregulation; cellular lipid metabolic process; fatty acid beta-oxidation using acyl-CoA dehydrogenase; negative regulation of fatty acid biosynthetic process; negative regulation of fatty acid oxidation; long-chain fatty acid catabolic process; protein homotetramerization
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 Research Articles on ACADL    1. the fatty acid oxidation pathway and LCAD are factors contributing to the pathophysiology of pulmonary disease
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ACADL elisa kitPathways associated with ACADL elisa kit
 Reference Product  PubMed Publications
 ACADM elisa kit  >5 publications with ACADL and ACADM
 CPT1B elisa kit  >4 publications with ACADL and CPT1B
 CPT1A elisa kit  >2 publications with ACADL and CPT1A
 EHHADH elisa kit  >2 publications with ACADL and EHHADH
 HADHA elisa kit  >1 publications with ACADL and HADHA
 ACAA2 elisa kit  >1 publications with ACADL and ACAA2
 HADHB elisa kit  >1 publications with ACADL and HADHB
 Products by Pathway  Pathway Diagram
 Beta Oxidation Of Lauroyl-CoA To Decanoyl-CoA-CoA Pathway antibodies  Beta Oxidation Of Lauroyl-CoA To Decanoyl-CoA-CoA Pathway Diagram
 Beta Oxidation Of Myristoyl-CoA To Lauroyl-CoA Pathway antibodies  Beta Oxidation Of Myristoyl-CoA To Lauroyl-CoA Pathway Diagram
 Fatty Acid Beta Oxidation Pathway antibodies  Fatty Acid Beta Oxidation Pathway Diagram
 Fatty Acid Degradation Pathway antibodies  Fatty Acid Degradation Pathway Diagram
 Fatty Acid Degradation Pathway antibodies  Fatty Acid Degradation Pathway Diagram
 Fatty Acid Metabolism Pathway antibodies  Fatty Acid Metabolism Pathway Diagram
 Fatty Acid Metabolism Pathway antibodies  Fatty Acid Metabolism Pathway Diagram
 Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway antibodies  Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
Diseases associated with ACADL elisa kitOrgans/Tissues associated with ACADL elisa kit
 Disease Name  Pubmed Publications
 Fatty Liver Antibodies  >8 publications with ACADL and Fatty Liver
 Liver Diseases Antibodies  >7 publications with ACADL and Liver Diseases
 Weight Gain Antibodies  >1 publications with ACADL and Weight Gain
 Neoplasms, Experimental Antibodies  >1 publications with ACADL and Neoplasms, Experimental
 Learning Disorders Antibodies  >1 publications with ACADL and Learning Disorders
 Nervous System Diseases Antibodies  >1 publications with ACADL and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >1 publications with ACADL and Cardiovascular Diseases
 Carcinoma, Hepatocellular Antibodies  >1 publications with ACADL and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >1 publications with ACADL and Liver Neoplasms
 Necrosis Antibodies  >1 publications with ACADL and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >10 publications with ACADL and Liver
 Connective Tissue Antibodies  >5 publications with ACADL and Connective Tissue
 Pancreas Antibodies  >4 publications with ACADL and Pancreas
 Muscle Antibodies  >4 publications with ACADL and Muscle
 Prostate Antibodies  >2 publications with ACADL and Prostate
 Bone Antibodies  >1 publications with ACADL and Bone
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