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LRP4 elisa kit :: Human Low Density Lipoprotein Receptor Related Protein 4 (LRP4) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2021747 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $270 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $665 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,715 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,895 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Low Density Lipoprotein Receptor Related Protein 4 (LRP4), ELISA Kit

 Also Known As   

Low Density Lipoprotein Receptor Related Protein 4 (LRP4) ELISA Kit

 Product Synonym Names    MEGF7; LRP10; Multiple epidermal growth factor-like domains 7
 Product Gene Name   

LRP4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    212780
 3D Structure    ModBase 3D Structure for O75096
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Low Density Lipoprotein Receptor Related Protein 4 (LRP4).
No significant cross-reactivity or interference between Low Density Lipoprotein Receptor Related Protein 4 (LRP4) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Tissue homogenates, Cell lysates and Other Biological Fluids
 Detection Range    0.312-20ng/mL
 Sensitivity    < 0.126ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Low Density Lipoprotein Receptor Related Protein 4 (LRP4) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Low Density Lipoprotein Receptor Related Protein 4 (LRP4) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of LRP4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for LRP4 purchase    MBS2021747 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Low Density Lipoprotein Receptor Related Protein 4 (LRP4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312-20ng/mL in biological research samples containing LRP4, with an estimated sensitivity of < 0.126ng/mL. The ELISA analytical biochemical technique of the MBS2021747 kit is based on LRP4 antibody-LRP4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LRP4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LRP4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates, Cell lysates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for LRP4 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Low Density Lipoprotein Receptor Related Protein 4 (LRP4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Low Density Lipoprotein Receptor Related Protein 4 (LRP4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Low Density Lipoprotein Receptor Related Protein 4 (LRP4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Low Density Lipoprotein Receptor Related Protein 4 (LRP4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for LRP4 elisa kit    Metabolic pathway
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 Typical Testing Data/Standard Curve (for reference only) of LRP4 elisa kit    LRP4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2021747. Click to request current manual
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NCBI/Uniprot data below describe general gene information for LRP4. It may not necessarily be applicable to this product.
 NCBI GI #    157384998
 NCBI GeneID    4038
 NCBI Accession #    NP_002325.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_002334.3 [Other Products]
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 UniProt Primary Accession #    O75096 [Other Products]
 UniProt Secondary Accession #    Q4AC85; Q5KTZ5; B2RN39 [Other Products]
 UniProt Related Accession #    O75096 [Other Products]
 Molecular Weight    212,045 Da
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 NCBI Official Full Name    low-density lipoprotein receptor-related protein 4
 NCBI Official Synonym Full Names    low density lipoprotein receptor-related protein 4
 NCBI Official Symbol    LRP4 [Similar Products]
 NCBI Official Synonym Symbols   
CLSS; CMS17; LRP-4; LRP10; MEGF7; SOST2
[Similar Products]
 NCBI Protein Information    low-density lipoprotein receptor-related protein 4
 UniProt Protein Name    Low-density lipoprotein receptor-related protein 4
 UniProt Synonym Protein Names   
Multiple epidermal growth factor-like domains 7
 Protein Family    Low-density lipoprotein receptor-related protein
 UniProt Gene Name    LRP4 [Similar Products]
 UniProt Synonym Gene Names    KIAA0816; LRP10; MEGF7; LRP-4 [Similar Products]
 UniProt Entry Name    LRP4_HUMAN
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 NCBI Summary for LRP4    This gene encodes a member of the low-density lipoprotein receptor-related protein family. The encoded protein may be a regulator of Wnt signaling. Mutations in this gene are associated with Cenani-Lenz syndrome. [provided by RefSeq, May 2010]
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 UniProt Comments for LRP4    LRP4: Mediates SOST-dependent inhibition of bone formation. Functions as a specific facilitator of SOST-mediated inhibition of Wnt signaling. Plays a key role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between motor neuron and skeletal muscle. Directly binds AGRIN and recruits it to the MUSK signaling complex. Mediates the AGRIN- induced phosphorylation of MUSK, the kinase of the complex. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Alternatively, may be involved in the negative regulation of the canonical Wnt signaling pathway, being able to antagonize the LRP6-mediated activation of this pathway. More generally, has been proposed to function as a cell surface endocytic receptor binding and internalizing extracellular ligands for degradation by lysosomes. Defects in LRP4 are the cause of Cenani-Lenz syndactyly syndrome (CLSS). It is a congenital malformation syndrome defined as complete and complex syndactyly of the hands combined with malformations of the forearm bones and similar manifestations in the lower limbs. Defects in LRP4 are the cause of sclerosteosis type 2 (SOST2). A sclerosing bone dysplasia characterized by a generalized hyperostosis and sclerosis leading to a markedly thickened skull, with mandible, ribs, clavicles and all long bones also being affected. Due to narrowing of the foramina of the cranial nerves, facial nerve palsy, hearing loss and atrophy of the optic nerves can occur. Sclerosteosis is clinically and radiologically very similar to van Buchem disease, mainly differentiated by hand malformations and a large stature in sclerosteosis patients. Belongs to the LDLR family.

Protein type: Cell surface; Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: cell surface; cell soma; dendrite; postsynaptic density; integral to membrane; flotillin complex; neuromuscular junction

Molecular Function: protein binding; protein homodimerization activity; apolipoprotein binding; calcium ion binding; receptor tyrosine kinase binding

Biological Process: limb development; regulation of protein amino acid phosphorylation; extracellular matrix organization and biogenesis; Wnt receptor signaling pathway; dendrite morphogenesis; endocytosis; odontogenesis of dentine-containing teeth; hair follicle development; synaptic growth at neuromuscular junction; dorsal/ventral pattern formation; negative regulation of ossification; synapse organization and biogenesis; protein heterotetramerization; negative regulation of axonogenesis; embryonic digit morphogenesis; kidney development; proximal/distal pattern formation

Disease: Myasthenic Syndrome, Congenital, 17; Cenani-lenz Syndactyly Syndrome; Sclerosteosis 2
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 Research Articles on LRP4    1. study presents 2 sibling fetuses with a prenatal lethal presentation of mesomelic limb reductions, oligosyndactyly, genitourinary malformation and compound heterozygosity for 2 novel truncating mutations in LRP4
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with LRP4 elisa kitPathways associated with LRP4 elisa kit
 Reference Product  PubMed Publications
 SOST elisa kit  >17 publications with LRP4 and SOST
 MSX1 elisa kit  >1 publications with LRP4 and MSX1
 AGRN elisa kit  >1 publications with LRP4 and AGRN
 LMX1B elisa kit  >1 publications with LRP4 and LMX1B
 WNT7A elisa kit  >1 publications with LRP4 and WNT7A
 Products by Pathway  Pathway Diagram
 ECM Proteoglycans Pathway antibodies  ECM Proteoglycans Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
Diseases associated with LRP4 elisa kitOrgans/Tissues associated with LRP4 elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >40 publications with LRP4 and Nervous System Diseases
 Syndactyly Antibodies  >12 publications with LRP4 and Syndactyly
 Atrophy Antibodies  >7 publications with LRP4 and Atrophy
 Disease Models, Animal Antibodies  >7 publications with LRP4 and Disease Models, Animal
 Craniofacial Abnormalities Antibodies  >5 publications with LRP4 and Craniofacial Abnormalities
 Tooth Abnormalities Antibodies  >4 publications with LRP4 and Tooth Abnormalities
 Inflammation Antibodies  >3 publications with LRP4 and Inflammation
 Hyperplasia Antibodies  >3 publications with LRP4 and Hyperplasia
 Abnormalities, Multiple Antibodies  >3 publications with LRP4 and Abnormalities, Multiple
 Brain Diseases Antibodies  >3 publications with LRP4 and Brain Diseases
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >68 publications with LRP4 and Muscle
 Nerve Antibodies  >23 publications with LRP4 and Nerve
 Blood Antibodies  >17 publications with LRP4 and Blood
 Embryonic Tissue Antibodies  >10 publications with LRP4 and Embryonic Tissue
 Brain Antibodies  >10 publications with LRP4 and Brain
 Connective Tissue Antibodies  >7 publications with LRP4 and Connective Tissue
 Thymus Antibodies  >5 publications with LRP4 and Thymus
 Heart Antibodies  >3 publications with LRP4 and Heart
 Kidney Antibodies  >3 publications with LRP4 and Kidney
 Lung Antibodies  >3 publications with LRP4 and Lung
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