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LY9 elisa kit :: Human Lymphocyte Antigen 9 (LY9) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2020709 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $260 +1 FREE 8GB USB
48-Strip-Wells  /  $475 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,590 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,670 +5 FREE 8GB USB
 
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 Product Name   

Lymphocyte Antigen 9 (LY9), ELISA Kit

 Also Known As   

Lymphocyte Antigen 9 (LY9) ELISA Kit

 Product Synonym Names    CD229; LY9; SLAMF3; Hly9; Mly9; SLAMF3; Cell surface molecule Ly-9; Signaling lymphocytic activation molecule 3
 Product Gene Name   

LY9 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    600684
 3D Structure    ModBase 3D Structure for Q9HBG7
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Lymphocyte Antigen 9 (LY9).
No significant cross-reactivity or interference between Lymphocyte Antigen 9 (LY9) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Tissue homogenates, Cell lysates and Other Biological Fluids
 Detection Range    0.312-20ng/mL
 Sensitivity    < 0.128ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Lymphocyte Antigen 9 (LY9) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Lymphocyte Antigen 9 (LY9) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of LY9 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for LY9 purchase    MBS2020709 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Lymphocyte Antigen 9 (LY9) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312-20ng/mL in biological research samples containing LY9, with an estimated sensitivity of < 0.128ng/mL. The ELISA analytical biochemical technique of the MBS2020709 kit is based on LY9 antibody-LY9 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LY9 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LY9. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates, Cell lysates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for LY9 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lymphocyte Antigen 9 (LY9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lymphocyte Antigen 9 (LY9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lymphocyte Antigen 9 (LY9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lymphocyte Antigen 9 (LY9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for LY9 elisa kit    Signal transduction; CD & Adhesion molecule; Tumor immunity; Infection immunity; Immune molecule
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 Typical Testing Data/Standard Curve (for reference only) of LY9 elisa kit    LY9 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2020709. Click to request current manual
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NCBI/Uniprot data below describe general gene information for LY9. It may not necessarily be applicable to this product.
 NCBI GI #    40555763
 NCBI GeneID    4063
 NCBI Accession #    AAH64485.1 [Other Products]
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 UniProt Primary Accession #    Q9HBG7 [Other Products]
 UniProt Secondary Accession #    Q14775; Q5VYI3; Q6P2J4; Q9H4N5; Q9NQ24; A8K7N3 [Other Products]
 UniProt Related Accession #    Q9HBG7 [Other Products]
 Molecular Weight    71,961 Da
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 NCBI Official Full Name    Lymphocyte antigen 9
 NCBI Official Synonym Full Names    lymphocyte antigen 9
 NCBI Official Symbol    LY9 [Similar Products]
 NCBI Official Synonym Symbols   
hly9; mLY9; CD229; SLAMF3
[Similar Products]
 NCBI Protein Information    T-lymphocyte surface antigen Ly-9
 UniProt Protein Name    T-lymphocyte surface antigen Ly-9
 UniProt Synonym Protein Names   
Cell surface molecule Ly-9; Lymphocyte antigen 9; SLAM family member 3; SLAMF3; Signaling lymphocytic activation molecule 3; CD_antigen: CD229
 Protein Family    T-lymphocyte surface antigen
 UniProt Gene Name    LY9 [Similar Products]
 UniProt Synonym Gene Names    SLAMF3 [Similar Products]
 UniProt Entry Name    LY9_HUMAN
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 NCBI Summary for LY9    LY9 belongs to the SLAM family of immunomodulatory receptors (see SLAMF1; MIM 603492) and interacts with the adaptor molecule SAP (SH2D1A; MIM 300490) (Graham et al., 2006 [PubMed 16365421]).[supplied by OMIM, Mar 2008]
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 UniProt Comments for LY9    CD229: May participate in adhesion reactions between T lymphocytes and accessory cells by homophilic interaction. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: integral to membrane

Molecular Function: receptor activity

Biological Process: cell adhesion
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 Research Articles on LY9    1. SLAMF3 is an inhibitor of hepatocellular carcinoma cell proliferation and tumor progression.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with LY9 elisa kitDiseases associated with LY9 elisa kit
 Reference Product  PubMed Publications
 SH2D1A elisa kit  >15 publications with LY9 and SH2D1A
 CD4 elisa kit  >6 publications with LY9 and CD4
 SH2D1B elisa kit  >2 publications with LY9 and SH2D1B
 SLAMF1 elisa kit  >1 publications with LY9 and SLAMF1
 PTPRC elisa kit  >1 publications with LY9 and PTPRC
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >2 publications with LY9 and Carcinoma
 Infant, Newborn, Diseases Antibodies  >2 publications with LY9 and Infant, Newborn, Diseases
 Liver Neoplasms Antibodies  >1 publications with LY9 and Liver Neoplasms
 Liver Diseases Antibodies  >1 publications with LY9 and Liver Diseases
 Cardiovascular Diseases Antibodies  >1 publications with LY9 and Cardiovascular Diseases
 Neoplasms, Experimental Antibodies  >1 publications with LY9 and Neoplasms, Experimental
 Necrosis Antibodies  >1 publications with LY9 and Necrosis
Organs/Tissues associated with LY9 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >35 publications with LY9 and Blood
 Bone Marrow Antibodies  >7 publications with LY9 and Bone Marrow
 Spleen Antibodies  >3 publications with LY9 and Spleen
 Thymus Antibodies  >3 publications with LY9 and Thymus
 Lymph Node Antibodies  >2 publications with LY9 and Lymph Node
 Pharynx Antibodies  >1 publications with LY9 and Pharynx
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