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AOF1 elisa kit :: Human Lysine-specific histone demethylase 1B (AOF1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS9327045 AOF1 elisa kit
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $680 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,100 +3 FREE 8GB USB
10x96-Strip-Wells  /  $6,095 +7 FREE 8GB USB
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 Product Name   

Lysine-specific histone demethylase 1B (AOF1), ELISA Kit

★Popular Item★
 Also Known As   

Human Lysine-specific histone demethylase 1B (AOF1) ELISA Kit

 Product Synonym Names    amine oxidase (flavin containing) domain 1; RP1-298J15.1; AOF1; C6orf193; DKFZp686I0412; FLJ33898; FLJ34109; FLJ43328; LSD2; bA204B7.3; dJ298J15.2; amine oxidase (flavin containing) domain 1|amine oxidase; flavin containing 1
 Product Gene Name   

AOF1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 6; NC_000006.11 (18155619..18224084). Location: 6p22.3
 OMIM    613081
 3D Structure    ModBase 3D Structure for Q8NB78
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 Species Reactivity    Human
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 Specificity    No significant cross-reactivity or interference between this analyte and analogues is observed.
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 Assay Type    Sandwich
 Detection Range    0.625 ng/ml - 20 ng/ml.
 Sensitivity    0.1 ng/ml.
 Intra-assay Precision    Intra-assay CV (%) is less than 15%.
 Inter-assay Precision    Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean 100].
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of AOF1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for AOF1 purchase    MBS9327045 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Lysine-specific histone demethylase 1B (AOF1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.625 ng/ml - 20 ng/ml in biological research samples containing AOF1, with an estimated sensitivity of 0.1 ng/ml. The ELISA analytical biochemical technique of the MBS9327045 kit is based on AOF1 antibody-AOF1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AOF1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AOF1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for AOF1 elisa kit

   Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of KDM1B (hereafter termed "analyte") in undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
Sample Manual Insert of MBS9327045. Click to request current manual
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NCBI/Uniprot data below describe general gene information for AOF1. It may not necessarily be applicable to this product.
 NCBI GI #    116256451
 NCBI GeneID    221656
 NCBI Accession #    NP_694587.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_153042.3 [Other Products]
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 UniProt Primary Accession #    Q8NB78 [Other Products]
 UniProt Secondary Accession #    Q5TGV3; Q6AI15; Q6ZUU4; Q8N258; Q96EL7; A2A2C5; A2A2C6 [Other Products]
 UniProt Related Accession #    Q8NB78 [Other Products]
 Molecular Weight    92,098 Da
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 NCBI Official Full Name    lysine-specific histone demethylase 1B
 NCBI Official Synonym Full Names    lysine (K)-specific demethylase 1B
 NCBI Official Symbol    KDM1B [Similar Products]
 NCBI Official Synonym Symbols   
AOF1; LSD2; C6orf193; bA204B7.3; dJ298J15.2
[Similar Products]
 NCBI Protein Information    lysine-specific histone demethylase 1B; amine oxidase, flavin containing 1; lysine-specific histone demethylase 2; amine oxidase (flavin containing) domain 1; flavin-containing amine oxidase domain-containing protein 1
 UniProt Protein Name    Lysine-specific histone demethylase 1B
 UniProt Synonym Protein Names   
Flavin-containing amine oxidase domain-containing protein 1; Lysine-specific histone demethylase 2
 UniProt Gene Name    KDM1B [Similar Products]
 UniProt Synonym Gene Names    AOF1; C6orf193; LSD2 [Similar Products]
 UniProt Entry Name    KDM1B_HUMAN
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 NCBI Summary for AOF1    Flavin-dependent histone demethylases, such as KDM1B, regulate histone lysine methylation, an epigenetic mark that regulates gene expression and chromatin function (Karytinos et al., 2009 [PubMed 19407342]).[supplied by OMIM, Oct 2009]
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 UniProt Comments for AOF1    AOF1: Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri- methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys- 36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4. Belongs to the flavin monoamine oxidase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; EC 1.-.-.-

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: nucleoplasm; nucleus

Molecular Function: DNA binding; FAD binding; zinc ion binding; oxidoreductase activity

Biological Process: establishment and/or maintenance of chromatin architecture; genetic imprinting; regulation of transcription, DNA-dependent; transcription, DNA-dependent; DNA methylation during gametogenesis; multicellular organismal development
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 Research Articles on AOF1    1. Human LSD2/KDM1b/AOF1 regulates gene transcription by modulating intragenic H3K4me2 methylation.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with AOF1 elisa kitOrgans/Tissues associated with AOF1 elisa kit
 Disease Name  Pubmed Publications
 Pregnancy Complications Antibodies  >1 publications with AOF1 and Pregnancy Complications
 Hypertension Antibodies  >1 publications with AOF1 and Hypertension
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >1 publications with AOF1 and Muscle
 Prostate Antibodies  >1 publications with AOF1 and Prostate
 Heart Antibodies  >1 publications with AOF1 and Heart
 Thyroid Antibodies  >1 publications with AOF1 and Thyroid
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