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LOX elisa kit :: Human Lysyl Oxidase (LOX) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2023535 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Standard Curve/Testing Data
Unit / Price
24-Strip-Wells  /  $270 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $665 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,715 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,895 +6 FREE 8GB USB
 
 Product Name   

Lysyl Oxidase (LOX), ELISA Kit

★Popular Item★
 Also Known As   

Lysyl Oxidase (LOX) ELISA Kit

 Product Synonym Names    Protein-Lysine 6-Oxidase
 Product Gene Name   

LOX elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    153455
 3D Structure    ModBase 3D Structure for P28300
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Lysyl Oxidase (LOX).
No significant cross-reactivity or interference between Lysyl Oxidase (LOX) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
 Detection Range    0.156-10ng/mL
 Sensitivity    < 0.056ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Lysyl Oxidase (LOX) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Lysyl Oxidase (LOX) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of LOX elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for LOX purchase    MBS2023535 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Lysyl Oxidase (LOX) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing LOX, with an estimated sensitivity of < 0.056ng/mL. The ELISA analytical biochemical technique of the MBS2023535 kit is based on LOX antibody-LOX antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LOX antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LOX. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for LOX elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysyl Oxidase (LOX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lysyl Oxidase (LOX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lysyl Oxidase (LOX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lysyl Oxidase (LOX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for LOX elisa kit    Enzyme & Kinase
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 Typical Standard Curve/Testing Data of LOX elisa kit    LOX elisa kit Typical Standard Curve/Testing Data image
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NCBI/Uniprot data below describe general gene information for LOX. It may not necessarily be applicable to this product.
 NCBI GI #    257065
 NCBI GeneID    4015
 NCBI Accession #    AAB23549.1 [Other Products]
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 UniProt Primary Accession #    P28300 [Other Products]
 UniProt Secondary Accession #    Q5FWF0; B2R5Q3 [Other Products]
 UniProt Related Accession #    P28300 [Other Products]
 Molecular Weight    46,944 Da
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 NCBI Official Full Name    lysyl oxidase
 NCBI Official Synonym Full Names    lysyl oxidase
 NCBI Official Symbol    LOX [Similar Products]
 NCBI Protein Information    protein-lysine 6-oxidase
 UniProt Protein Name    Protein-lysine 6-oxidase
 UniProt Synonym Protein Names   
Lysyl oxidase
 Protein Family    Linoleate 9/13-lipoxygenase
 UniProt Gene Name    LOX [Similar Products]
 UniProt Entry Name    LYOX_HUMAN
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 NCBI Summary for LOX    The protein encoded by this gene is an extracellular copper enzyme that initiates the crosslinking of collagens and elastin. The enzyme catalyzes oxidative deamination of the epsilon-amino group in certain lysine and hydroxylysine residues of collagens and lysine residues of elastin. In addition to crosslinking extracellular matrix proteins, the encoded protein may have a role in tumor suppression. Defects in this gene are a cause of autosomal recessive cutis laxa type I (CL type I). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010]
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 UniProt Comments for LOX    LOX: Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. In addition to cross-linking of extracellular matrix proteins, may have a direct role in tumor suppression. Belongs to the lysyl oxidase family.

Protein type: Oxidoreductase; Secreted; EC 1.4.3.13; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q23.2

Cellular Component: proteinaceous extracellular matrix; extracellular space; collagen; extracellular region; nucleus

Molecular Function: protein binding; copper ion binding; protein-lysine 6-oxidase activity

Biological Process: response to drug; elastic fiber assembly; blood vessel development; extracellular matrix organization and biogenesis; collagen fibril organization; wound healing; response to steroid hormone stimulus; protein modification process; lung development

Disease: Cutis Laxa, Autosomal Recessive, Type Ia
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 Research Articles on LOX    1. LOX may play a role in the metastasis of non-small cell lung cancer by promoting MMP2/MMP9 expression. LOX expression is an independent prognostic factor for survival in NSCLC.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with LOX elisa kitPathways associated with LOX elisa kit
 Reference Product  PubMed Publications
 ALOX5 elisa kit  >364 publications with LOX and ALOX5
 SRC elisa kit  >30 publications with LOX and SRC
 FGF2 elisa kit  >13 publications with LOX and FGF2
 FBLN5 elisa kit  >7 publications with LOX and FBLN5
 FBN1 elisa kit  >4 publications with LOX and FBN1
 TGFBI elisa kit  >2 publications with LOX and TGFBI
 FN1 elisa kit  >2 publications with LOX and FN1
 ELN elisa kit  >1 publications with LOX and ELN
 Products by Pathway  Pathway Diagram
 Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway antibodies  Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway Diagram
 Collagen Formation Pathway antibodies  Collagen Formation Pathway Diagram
 Crosslinking Of Collagen Fibrils Pathway antibodies  Crosslinking Of Collagen Fibrils Pathway Diagram
 Elastic Fibre Formation Pathway antibodies  Elastic Fibre Formation Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
Diseases associated with LOX elisa kitOrgans/Tissues associated with LOX elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >580 publications with LOX and Inflammation
 Cardiovascular Diseases Antibodies  >563 publications with LOX and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >374 publications with LOX and Disease Models, Animal
 Necrosis Antibodies  >227 publications with LOX and Necrosis
 Heart Diseases Antibodies  >174 publications with LOX and Heart Diseases
 Brain Diseases Antibodies  >156 publications with LOX and Brain Diseases
 Neoplasms, Experimental Antibodies  >153 publications with LOX and Neoplasms, Experimental
 Hypertension Antibodies  >140 publications with LOX and Hypertension
 Fibrosis Antibodies  >126 publications with LOX and Fibrosis
 Lung Neoplasms Antibodies  >116 publications with LOX and Lung Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >703 publications with LOX and Vascular
 Muscle Antibodies  >386 publications with LOX and Muscle
 Brain Antibodies  >364 publications with LOX and Brain
 Lung Antibodies  >278 publications with LOX and Lung
 Heart Antibodies  >251 publications with LOX and Heart
 Liver Antibodies  >239 publications with LOX and Liver
 Bone Antibodies  >219 publications with LOX and Bone
 Skin Antibodies  >178 publications with LOX and Skin
 Kidney Antibodies  >159 publications with LOX and Kidney
 Embryonic Tissue Antibodies  >153 publications with LOX and Embryonic Tissue
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