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MIF elisa kit :: Human MIF ELISA Kit

Scan QR to view Datasheet Catalog #    MBS355376
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96 Tests  /  $540 +1 FREE 8GB USB
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 Product Name   


★Popular Item★
 Also Known As   


 Product Synonym Names    Human MIF
 Product Gene Name   

MIF elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 22; NC_000022.10 (24236565..24237409). Location: 22q11.23
 OMIM    153620
 3D Structure    ModBase 3D Structure for P14174
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 Species Reactivity    Human
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 Assay Type    Sandwich
 Detection Range    156 pg/ml-10,000 pg/ml
 Sensitivity    < 20pg/ml
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 Preparation and Storage    Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of MIF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MIF purchase    MBS355376 is a ready-to-use microwell, strip-or-full plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the MIF, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 156 pg/ml-10,000 pg/ml in biological research samples containing MIF, with an estimated sensitivity of < 20pg/ml. The ELISA analytical biochemical technique of the MBS355376 kit is based on MIF antibody-MIF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MIF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MIF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for MIF elisa kit

   Background/Introduction: Macrophage migration inhibitory factor (MIF or MMIF) also known as glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, or phenylpyruvate tautomerase is a protein that in humans is encoded by the MIF gene. Macrophage migration inhibitory factor assembles into a trimer composed of three identical subunits. Each of these monomers contain two antiparallel alpha helices and a four-stranded beta sheet. The monomers surround a central channel with 3-fold rotational symmetry. MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. It is an inflammatory mediator associated with rheumatoid arthritis (RA) severity. Additionally, evidence suggests that there is a correlation between MIF production and metastatic potential in colorectal cancer.

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-MIF polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-MIF polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MIF amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MIF can be calculated.
 Applications Tested/Suitable for MIF elisa kit   

Quantitative sELISA (EIA)

 Application Notes for MIF elisa kit    For quantitative detection of MIF in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
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 Typical Testing Data/Standard Curve (for reference only) of MIF elisa kit    MIF elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS355376. Click to request current manual
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NCBI/Uniprot data below describe general gene information for MIF. It may not necessarily be applicable to this product.
 NCBI GI #    47678571
 NCBI GeneID    4282
 NCBI Accession #    CAG30406.1 [Other Products]
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 UniProt Primary Accession #    P14174 [Other Products]
 UniProt Secondary Accession #    Q2V4Y5; Q6FHV0; A5Z1R8; B2R4S3 [Other Products]
 UniProt Related Accession #    P14174 [Other Products]
 Molecular Weight    12,476 Da
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 NCBI Official Full Name    MIF
 NCBI Official Synonym Full Names    macrophage migration inhibitory factor (glycosylation-inhibiting factor)
 NCBI Official Symbol    MIF [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    macrophage migration inhibitory factor; L-dopachrome isomerase; L-dopachrome tautomerase; phenylpyruvate tautomerase
 UniProt Protein Name    Macrophage migration inhibitory factor
 UniProt Synonym Protein Names   
Glycosylation-inhibiting factor; GIF; L-dopachrome isomerase; L-dopachrome tautomerase (EC:; Phenylpyruvate tautomerase
 Protein Family    MIF-like protein
 UniProt Gene Name    MIF [Similar Products]
 UniProt Synonym Gene Names    GLIF; MMIF; MIF; GIF [Similar Products]
 UniProt Entry Name    MIF_HUMAN
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 NCBI Summary for MIF    This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008]
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 UniProt Comments for MIF    Function: Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity. Ref.23 Ref.24

Catalytic activity: Keto-phenylpyruvate = enol-phenylpyruvate. Ref.32 Ref.34L-dopachrome = 5,6-dihydroxyindole-2-carboxylate. Ref.32 Ref.34

Subunit structure: Homotrimer. Interacts with CXCR2 extracellular domain

By similarity. Interacts with the CD74 extracellular domain, COPS5 and BNIPL. Ref.18 Ref.21 Ref.22 Ref.25 Ref.33 Ref.34

Subcellular location: Secreted. Cytoplasm. Note: Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens. Ref.1 Ref.2 Ref.18 Ref.23 Ref.25

Induction: Up-regulated in concanavalin-A-treated lymphocytes. Up-regulated in macrophages upon exposure to M.tuberculosis antigens. Ref.1 Ref.23

Involvement in disease: Rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]: An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.Note: Disease susceptibility is associated with variations affecting the gene represented in this entry.

Miscellaneous: Serum levels of MIF are elevated in patients with severe sepsis or septic shock. High levels of MIF are correlated with low survival. Drugs that inhibit tautomerase activity protect against death due to sepsis.

Sequence similarities: Belongs to the MIF family.

Biophysicochemical propertiesKinetic parameters:KM=249 µM for phenylpyruvate Ref.20KM=168 µM for p-hydroxyphenylpyruvateVmax=2113 µmol/min/mg enzyme toward phenylpyruvateVmax=524 µmol/min/mg enzyme toward p-hydroxyphenylpyruvate
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 Research Articles on MIF    1. Mutant N110C can form a higher-order oligomer in equilibrium with a single locked trimer. The X-ray structure confirms a local conformational change that disrupts the subunit interface and results in global changes responsible for the oligomeric form.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with MIF elisa kitPathways associated with MIF elisa kit
 Reference Product  PubMed Publications
 CD74 elisa kit  >109 publications with MIF and CD74
 COPS5 elisa kit  >17 publications with MIF and COPS5
 MMP9 elisa kit  >10 publications with MIF and MMP9
 S100A8 elisa kit  >4 publications with MIF and S100A8
 TAT elisa kit  >1 publications with MIF and TAT
 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Phenylalanine Metabolism Pathway antibodies  Phenylalanine Metabolism Pathway Diagram
 Phenylalanine Metabolism Pathway antibodies  Phenylalanine Metabolism Pathway Diagram
 Spinal Cord Injury Pathway antibodies  Spinal Cord Injury Pathway Diagram
 Tyrosine Metabolism Pathway antibodies  Tyrosine Metabolism Pathway Diagram
 Tyrosine Metabolism Pathway antibodies  Tyrosine Metabolism Pathway Diagram
Diseases associated with MIF elisa kitOrgans/Tissues associated with MIF elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >620 publications with MIF and Inflammation
 Neoplasms Antibodies  >429 publications with MIF and Neoplasms
 Necrosis Antibodies  >303 publications with MIF and Necrosis
 Disease Models, Animal Antibodies  >210 publications with MIF and Disease Models, Animal
 Lung Diseases Antibodies  >200 publications with MIF and Lung Diseases
 Carcinoma Antibodies  >145 publications with MIF and Carcinoma
 Sepsis Antibodies  >130 publications with MIF and Sepsis
 Arthritis, Rheumatoid Antibodies  >118 publications with MIF and Arthritis, Rheumatoid
 Heart Diseases Antibodies  >90 publications with MIF and Heart Diseases
 Pneumonia Antibodies  >79 publications with MIF and Pneumonia
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1436 publications with MIF and Blood
 Brain Antibodies  >389 publications with MIF and Brain
 Vascular Antibodies  >216 publications with MIF and Vascular
 Skin Antibodies  >207 publications with MIF and Skin
 Lung Antibodies  >195 publications with MIF and Lung
 Muscle Antibodies  >183 publications with MIF and Muscle
 Spleen Antibodies  >164 publications with MIF and Spleen
 Liver Antibodies  >151 publications with MIF and Liver
 Heart Antibodies  >128 publications with MIF and Heart
 Lymph Antibodies  >122 publications with MIF and Lymph
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