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MMP8 elisa kit :: Human MMP8 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS824833
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96 Tests  /  $470 +1 FREE 8GB USB
 
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 Product Name   

MMP8, ELISA Kit

★Popular Item★
 Also Known As   

Human MMP8 ELISA Kit

 Product Synonym Names    CLG1; Neutrophil collagenase; Matrix metalloproteinase-8; MMP-8; PMNL collagenase; PMNL-CL
 Product Gene Name   

MMP8 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    120355
 3D Structure    ModBase 3D Structure for P22894
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 Species Reactivity    Human
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 Specificity    The Human MMP8 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human MMP8 proteins within the range of 156 pg/ml - 10000 pg/ml.
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 Samples    Cell Culture Supernatants, Serum, Plasma
 Sensitivity    The minimum detectable dose of Human MMP8 is typically less than 10 pg/ml.
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 Preparation and Storage    Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of MMP8 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MMP8 purchase    MBS824833 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the MMP8, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MMP8, with an estimated sensitivity of The minimum detectable dose of Human MMP8 is typically less than 10 pg/ml. The ELISA analytical biochemical technique of the MBS824833 kit is based on MMP8 antibody-MMP8 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MMP8 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MMP8. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell Culture Supernatants, Serum, Plasma. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for MMP8 elisa kit

   Principle of the assay: The Bioscience Human MMP8 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MMP8 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human MMP8 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP8 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP8 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP8 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Matrix metalloproteinase 8 (MMP8) also called neutrophil collagenase. Neutrophil collagenase, a member of the family of matrix metalloproteinases, is distinct from the collagenase of skin fibroblasts and synovial cells in substrate specificity and immunologic crossreactivity. MMP8, an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells. The human neutrophil collagenase (HNC) cDNA clone has been sequenced and shown to encode a 467-residue protein. Neutrophil collagenase has been found to possess 57% identity with the deduced protein sequence for fibroblast collagenase with 72% chemical similarity. Certain regions of the molecule, including the putative zinc-binding region, are highly conserved. When compared with the published sequence for fibroblast collagenase, neutrophil collagenase contains four additional sites for glycosylation.
 Applications Tested/Suitable for MMP8 elisa kit   

Sandwich ELISA (SE)

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 Typical Testing Data/Standard Curve (for reference only) of MMP8 elisa kit    MMP8 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS824833. Click to request current manual
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NCBI/Uniprot data below describe general gene information for MMP8. It may not necessarily be applicable to this product.
 NCBI GI #    4505221
 NCBI GeneID    4317
 NCBI Accession #    NP_002415.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_002424.2 [Other Products]
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 UniProt Primary Accession #    P22894 [Other Products]
 UniProt Secondary Accession #    Q45F99 [Other Products]
 UniProt Related Accession #    P22894 [Other Products]
 Molecular Weight    53,412 Da
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 NCBI Official Full Name    neutrophil collagenase preproprotein
 NCBI Official Synonym Full Names    matrix metallopeptidase 8 (neutrophil collagenase)
 NCBI Official Symbol    MMP8 [Similar Products]
 NCBI Official Synonym Symbols   
HNC; CLG1; MMP-8; PMNL-CL
[Similar Products]
 NCBI Protein Information    neutrophil collagenase; PMNL collagenase; matrix metalloproteinase-8; matrix metalloproteinase 8 (neutrophil collagenase)
 UniProt Protein Name    Neutrophil collagenase
 UniProt Synonym Protein Names   
Matrix metalloproteinase-8; MMP-8; PMNL collagenase; PMNL-CL
 Protein Family    Neutrophil collagenase
 UniProt Gene Name    MMP8 [Similar Products]
 UniProt Synonym Gene Names    CLG1; MMP-8; PMNL-CL [Similar Products]
 UniProt Entry Name    MMP8_HUMAN
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 NCBI Summary for MMP8    Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by this gene is stored in secondary granules within neutrophils and is activated by autolytic cleavage. Its function is degradation of type I, II and III collagens. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]
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 UniProt Comments for MMP8    MMP8: Can degrade fibrillar type I, II, and III collagens. Belongs to the peptidase M10A family.

Protein type: EC 3.4.24.34; Protease; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region

Molecular Function: zinc ion binding; serine-type endopeptidase activity; metalloendopeptidase activity; calcium ion binding

Biological Process: collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; proteolysis
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 Research Articles on MMP8    1. MMP8 rs1940475 SNP modifies the host response to inflammatory stimuli.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with MMP8 elisa kitPathways associated with MMP8 elisa kit
 Reference Product  PubMed Publications
 TIMP1 elisa kit  >25 publications with MMP8 and TIMP1
 TIMP2 elisa kit  >11 publications with MMP8 and TIMP2
 CCL2 elisa kit  >6 publications with MMP8 and CCL2
 PTGS2 elisa kit  >2 publications with MMP8 and PTGS2
 EFNB1 elisa kit  >1 publications with MMP8 and EFNB1
 Products by Pathway  Pathway Diagram
 Activation Of Matrix Metalloproteinases Pathway antibodies  Activation Of Matrix Metalloproteinases Pathway Diagram
 Collagen Degradation Pathway antibodies  Collagen Degradation Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Matrix Metalloproteinases Pathway antibodies  Matrix Metalloproteinases Pathway Diagram
Diseases associated with MMP8 elisa kitOrgans/Tissues associated with MMP8 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >73 publications with MMP8 and Inflammation
 Cardiovascular Diseases Antibodies  >34 publications with MMP8 and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >26 publications with MMP8 and Disease Models, Animal
 Fibrosis Antibodies  >26 publications with MMP8 and Fibrosis
 Lung Diseases Antibodies  >17 publications with MMP8 and Lung Diseases
 Hemorrhage Antibodies  >16 publications with MMP8 and Hemorrhage
 Atherosclerosis Antibodies  >14 publications with MMP8 and Atherosclerosis
 Necrosis Antibodies  >12 publications with MMP8 and Necrosis
 Wounds and Injuries Antibodies  >12 publications with MMP8 and Wounds and Injuries
 Heart Diseases Antibodies  >11 publications with MMP8 and Heart Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >77 publications with MMP8 and Blood
 Connective Tissue Antibodies  >15 publications with MMP8 and Connective Tissue
 Skin Antibodies  >13 publications with MMP8 and Skin
 Liver Antibodies  >10 publications with MMP8 and Liver
 Bone Marrow Antibodies  >9 publications with MMP8 and Bone Marrow
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