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MUC20 elisa kit :: Human MUC20 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2508173
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

MUC20, ELISA Kit

★Popular Item★
 Also Known As   

Human MUC20 (Mucin 20) ELISA Kit

 Product Gene Name   

MUC20 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    610360
 3D Structure    ModBase 3D Structure for Q8N307
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 Species Reactivity    Human
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 Specificity    This kit recognizes natural and recombinant Human MUC20. No significant cross-reactivity or interference between Human MUC20 and analogues was observed.
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 Samples    Serum, plasma and other biological fluids
 Assay Type    Sandwich
 Detection Range    0.156-10ng/mL
 Sensitivity    The minimum detectable dose of Human MUC20 is 0.094ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
 Intended Uses    This ELISA kit applies to the in vitro quantitative determination of Human MUC20 concentrations in serum, plasma and other biological fluids.
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 Preparation and Storage    Store at 4 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of MUC20 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MUC20 purchase    MBS2508173 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the MUC20, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing MUC20, with an estimated sensitivity of The minimum detectable dose of Human MUC20 is 0.094ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero). The ELISA analytical biochemical technique of the MBS2508173 kit is based on MUC20 antibody-MUC20 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MUC20 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MUC20. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for MUC20 elisa kit

   Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MUC20. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MUC20 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MUC20, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of MUC20. You can calculate the concentration of MUC20 in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of MUC20 elisa kit    MUC20 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2508173. Click to request current manual
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NCBI/Uniprot data below describe general gene information for MUC20. It may not necessarily be applicable to this product.
 NCBI GI #    541444092
 NCBI GeneID    200958
 NCBI Accession #    NP_001269435.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001282506.1 [Other Products]
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 UniProt Primary Accession #    Q8N307 [Other Products]
 UniProt Secondary Accession #    Q6UX97; Q76I83; Q76I85; Q86ST8; Q8NBY6; Q96KA1; Q9P2I8 [Other Products]
 UniProt Related Accession #    Q8N307 [Other Products]
 Molecular Weight    68,308 Da
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 NCBI Official Full Name    mucin-20 isoform a
 NCBI Official Synonym Full Names    mucin 20, cell surface associated
 NCBI Official Symbol    MUC20 [Similar Products]
 NCBI Official Synonym Symbols   
MUC-20
[Similar Products]
 NCBI Protein Information    mucin-20; transmembrane mucin MUC20S
 UniProt Protein Name    Mucin-20
 Protein Family    Mucin
 UniProt Gene Name    MUC20 [Similar Products]
 UniProt Synonym Gene Names    KIAA1359; MUC-20 [Similar Products]
 UniProt Entry Name    MUC20_HUMAN
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 NCBI Summary for MUC20    This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins secreted by many epithelial tissues to form an insoluble mucous barrier. The C-terminus of this family member associates with the multifunctional docking site of the MET proto-oncogene and suppresses activation of some downstream MET signaling cascades. The protein features a mucin tandem repeat domain that varies between two and six copies in most individuals. Multiple variants encoding different isoforms have been found for this gene. A related pseudogene, which is also located on chromosome 3, has been identified. [provided by RefSeq, Apr 2014]
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 UniProt Comments for MUC20    MUC20: May regulate MET signaling cascade. Seems to decrease hepatocyte growth factor (HGF)-induced transient MAPK activation. Blocks GRB2 recruitment to MET thus suppressing the GRB2-RAS pathway. Inhibits HGF-induced proliferation of MMP1 and MMP9 expression. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: Golgi lumen; microvillus membrane; apical plasma membrane; basal plasma membrane; extracellular region

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; activation of MAPK activity; hepatocyte growth factor receptor signaling pathway; post-translational protein modification; protein homooligomerization
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 Research Articles on MUC20    1. MUC20 is a novel transmembrane mucin expressed by the human corneal and conjunctival epithelia, and suggest that differential expression of MUC20 during differentiation has a role in maintaining ocular surface homeostasis.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with MUC20 elisa kitPathways associated with MUC20 elisa kit
 Reference Product  PubMed Publications
 MUC4 elisa kit  >13 publications with MUC20 and MUC4
 MUC13 elisa kit  >10 publications with MUC20 and MUC13
 MUC5B elisa kit  >8 publications with MUC20 and MUC5B
 MUC2 elisa kit  >6 publications with MUC20 and MUC2
 MUC19 elisa kit  >4 publications with MUC20 and MUC19
 MUC15 elisa kit  >4 publications with MUC20 and MUC15
 MUC7 elisa kit  >3 publications with MUC20 and MUC7
 MET elisa kit  >1 publications with MUC20 and MET
 MUC3A elisa kit  >1 publications with MUC20 and MUC3A
 Products by Pathway  Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 O-linked Glycosylation Pathway antibodies  O-linked Glycosylation Pathway Diagram
 O-linked Glycosylation Of Mucins Pathway antibodies  O-linked Glycosylation Of Mucins Pathway Diagram
 Post-translational Protein Modification Pathway antibodies  Post-translational Protein Modification Pathway Diagram
 Signaling Events Mediated By Hepatocyte Growth Factor Receptor (c-Met) Pathway antibodies  Signaling Events Mediated By Hepatocyte Growth Factor Receptor (c-Met) Pathway Diagram
 Termination Of O-glycan Biosynthesis Pathway antibodies  Termination Of O-glycan Biosynthesis Pathway Diagram
Diseases associated with MUC20 elisa kitOrgans/Tissues associated with MUC20 elisa kit
 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >5 publications with MUC20 and Kidney Diseases
 Inflammation Antibodies  >4 publications with MUC20 and Inflammation
 Necrosis Antibodies  >2 publications with MUC20 and Necrosis
 Liver Diseases Antibodies  >1 publications with MUC20 and Liver Diseases
 Uterine Diseases Antibodies  >1 publications with MUC20 and Uterine Diseases
 Hypertension Antibodies  >1 publications with MUC20 and Hypertension
 Hyperplasia Antibodies  >1 publications with MUC20 and Hyperplasia
 Placenta Diseases Antibodies  >1 publications with MUC20 and Placenta Diseases
 Organ/Tissue Name  Pubmed Publications
 Intestine Antibodies  >6 publications with MUC20 and Intestine
 Kidney Antibodies  >4 publications with MUC20 and Kidney
 Prostate Antibodies  >3 publications with MUC20 and Prostate
 Lung Antibodies  >3 publications with MUC20 and Lung
 Bladder Antibodies  >3 publications with MUC20 and Bladder
 Epididymis Antibodies  >2 publications with MUC20 and Epididymis
 Eye Antibodies  >2 publications with MUC20 and Eye
 Liver Antibodies  >1 publications with MUC20 and Liver
 Trachea Antibodies  >1 publications with MUC20 and Trachea
 Brain Antibodies  >1 publications with MUC20 and Brain
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