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MUC5B elisa kit :: Human MUC5B ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2507356
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   


★Popular Item★
 Also Known As   

Human MUC5B (Mucin 5 Subtype B) ELISA Kit

 Product Gene Name   

MUC5B elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2507356 COA    COA PDF
 OMIM    178500
 3D Structure    ModBase 3D Structure for Q9HC84
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 Species Reactivity    Human
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 Specificity    This kit recognizes natural and recombinant Human MUC5B. No significant cross-reactivity or interference between Human MUC5B and analogues was observed.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    0.625-40ng/mL
 Sensitivity    Min: 0.375ng/mL; Max: 40ng/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of MUC5B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MUC5B purchase    MBS2507356 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the MUC5B, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.625-40ng/mL in biological research samples containing MUC5B, with an estimated sensitivity of Min: 0.375ng/mL; Max: 40ng/mL. The ELISA analytical biochemical technique of the MBS2507356 kit is based on MUC5B antibody-MUC5B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MUC5B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MUC5B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for MUC5B elisa kit

   Intended Uses||This ELISA kit applies to the in vitro quantitative determination of Human MUC5B concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MUC5B. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MUC5B and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MUC5B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of MUC5B. You can calculate the concentration of MUC5B in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of MUC5B elisa kit    MUC5B elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2507356. Click to request current manual
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NCBI/Uniprot data below describe general gene information for MUC5B. It may not necessarily be applicable to this product.
 NCBI GI #    301172750
 NCBI GeneID    727897
 NCBI Accession #    NP_002449.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_002458.2 [Other Products]
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 UniProt Primary Accession #    Q9HC84 [Other Products]
 UniProt Secondary Accession #    O00447; O00573; O14985; O15494; O95291; O95451; Q14881; Q7M4S5; Q99552; Q9UE28 [Other Products]
 UniProt Related Accession #    Q9HC84 [Other Products]
 Molecular Weight    596,340 Da
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 NCBI Official Full Name    mucin-5B
 NCBI Official Synonym Full Names    mucin 5B, oligomeric mucus/gel-forming
 NCBI Official Symbol    MUC5B [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    mucin-5B; cervical mucin MUC5B; high molecular weight salivary mucin MG1; mucin 5, subtype B, tracheobronchial; sublingual gland mucin
 UniProt Protein Name    Mucin-5B
 UniProt Synonym Protein Names   
Cervical mucin; High molecular weight salivary mucin MG1; Mucin-5 subtype B, tracheobronchial; Sublingual gland mucin
 Protein Family    Mucin
 UniProt Gene Name    MUC5B [Similar Products]
 UniProt Synonym Gene Names    MUC5; MUC-5B [Similar Products]
 UniProt Entry Name    MUC5B_HUMAN
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 NCBI Summary for MUC5B    This gene encodes a member of the mucin family of proteins, which are highly glycosylated macromolecular components of mucus secretions. This family member is the major gel-forming mucin in mucus. It is a major contributor to the lubricating and viscoelastic properties of whole saliva, normal lung mucus and cervical mucus. This gene has been found to be up-regulated in some human diseases, including sinus mucosa of chronic rhinosinusitis (CRS), CRS with nasal polyposis, chronic obstructive pulmonary disease (COPD) and H. pylori-associated gastric disease, and it may be involved in the pathogenesis of these diseases. [provided by RefSeq, Jul 2010]
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 UniProt Comments for MUC5B    MUC5B: Gel-forming mucin that is thought to contribute to the lubricating and viscoelastic properties of whole saliva and cervical mucus. Defects in MUC5B are a cause of susceptibility to pulmonary fibrosis idiopathic (IPF). Pulmonary fibrosis is a lung disease characterized by shortness of breath, radiographically evident diffuse pulmonary infiltrates, and varying degrees of inflammation and fibrosis on biopsy. It results in acute lung injury with subsequent scarring and endstage lung disease. A common polymorphism in the promoter of MUC5B is associated with familial interstitial pneumonia and idiopathic pulmonary fibrosis, suggesting that dysregulated MUC5B expression in the lung may be involved in the pathogenesis of pulmonary fibrosis (PubMed:21506741).

Protein type: Secreted; Secreted, signal peptide; Cell adhesion

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: extracellular space; Golgi lumen

Molecular Function: protein binding

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; regulation of macrophage activation; defense response to bacterium; post-translational protein modification

Disease: Pulmonary Fibrosis, Idiopathic
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 Research Articles on MUC5B    1. Adolescents with very high intensity of dental caries disease had increased levels of MUC1 and MUC5B.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with MUC5B elisa kitDiseases associated with MUC5B elisa kit
 Products by Pathway  Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 O-linked Glycosylation Pathway antibodies  O-linked Glycosylation Pathway Diagram
 O-linked Glycosylation Of Mucins Pathway antibodies  O-linked Glycosylation Of Mucins Pathway Diagram
 Post-translational Protein Modification Pathway antibodies  Post-translational Protein Modification Pathway Diagram
 Salivary Secretion Pathway antibodies  Salivary Secretion Pathway Diagram
 Salivary Secretion Pathway antibodies  Salivary Secretion Pathway Diagram
 Termination Of O-glycan Biosynthesis Pathway antibodies  Termination Of O-glycan Biosynthesis Pathway Diagram
 Disease Name  Pubmed Publications
 Lung Diseases Antibodies  >121 publications with MUC5B and Lung Diseases
 Inflammation Antibodies  >71 publications with MUC5B and Inflammation
 Idiopathic Pulmonary Fibrosis Antibodies  >36 publications with MUC5B and Idiopathic Pulmonary Fibrosis
 Immune System Diseases Antibodies  >35 publications with MUC5B and Immune System Diseases
 Hyperplasia Antibodies  >29 publications with MUC5B and Hyperplasia
 Necrosis Antibodies  >19 publications with MUC5B and Necrosis
 Lung Neoplasms Antibodies  >16 publications with MUC5B and Lung Neoplasms
 Respiration Disorders Antibodies  >11 publications with MUC5B and Respiration Disorders
 Ovarian Neoplasms Antibodies  >7 publications with MUC5B and Ovarian Neoplasms
 Ovarian Diseases Antibodies  >6 publications with MUC5B and Ovarian Diseases
Organs/Tissues associated with MUC5B elisa kit
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >159 publications with MUC5B and Lung
 Intestine Antibodies  >50 publications with MUC5B and Intestine
 Trachea Antibodies  >38 publications with MUC5B and Trachea
 Stomach Antibodies  >36 publications with MUC5B and Stomach
 Larynx Antibodies  >30 publications with MUC5B and Larynx
 Cervix Antibodies  >12 publications with MUC5B and Cervix
 Pancreas Antibodies  >12 publications with MUC5B and Pancreas
 Uterus Antibodies  >8 publications with MUC5B and Uterus
 Prostate Antibodies  >5 publications with MUC5B and Prostate
 Pharynx Antibodies  >2 publications with MUC5B and Pharynx
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