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PRAME elisa kit :: Human Melanoma antigen preferentially expressed in tumors ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2886190
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $380 +1 FREE 8GB USB
96-Strip-Wells  /  $500 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,170 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,840 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Melanoma antigen preferentially expressed in tumors (PRAME), ELISA Kit

★Popular Item★
 Also Known As   

Human Melanoma antigen preferentially expressed in tumors ELISA Kit

 Product Synonym Names    Melanoma antigen preferentially expressed in tumors; Opa-interacting protein 4; OIP-4; Preferentially expressed antigen of melanoma; PRAME; MAPE; OIP4
 Product Gene Name   

PRAME elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2886190 Testing Data    Testing Data PDF
 OMIM    606021
 3D Structure    ModBase 3D Structure for P78395
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 Species Reactivity    Human
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 Specificity    Recombinant and natural Human Melanoma antigen
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 Samples    serum, plasma, tissue homogenates?cell culture supernates or other biological fluids
 Detection Range    0.156-10 ng/mL
 Sensitivity    <0.056 ng/mL
 Intra-assay Precision    Intra-Assay CV: ?4.9%
 Inter-assay Precision    Inter-Assay CV: ?8.8%
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 Preparation and Storage    For long term storage, please store the entire kit at -20 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PRAME elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PRAME purchase    MBS2886190 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Melanoma antigen preferentially expressed in tumors (PRAME) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10 ng/mL in biological research samples containing PRAME, with an estimated sensitivity of <0.056 ng/mL. The ELISA analytical biochemical technique of the MBS2886190 kit is based on PRAME antibody-PRAME antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PRAME antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PRAME. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as serum, plasma, tissue homogenates?cell culture supernates or other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PRAME elisa kit

   Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates?cell culture supernates or other biological fluids.
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 Typical Testing Data/Standard Curve (for reference only) of PRAME elisa kit    PRAME elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2886190. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PRAME. It may not necessarily be applicable to this product.
 NCBI GI #    619328985
 NCBI GeneID    23532
 NCBI Accession #    NP_001278644.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001291715.1 [Other Products]
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 UniProt Primary Accession #    P78395 [Other Products]
 UniProt Secondary Accession #    O43481; Q8IXN8; B2R6Y7 [Other Products]
 UniProt Related Accession #    P78395 [Other Products]
 Molecular Weight    57,890 Da
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 NCBI Official Full Name    melanoma antigen preferentially expressed in tumors isoform a
 NCBI Official Synonym Full Names    preferentially expressed antigen in melanoma
 NCBI Official Symbol    PRAME [Similar Products]
 NCBI Official Synonym Symbols   
MAPE; OIP4; CT130; OIP-4
[Similar Products]
 NCBI Protein Information    melanoma antigen preferentially expressed in tumors
 UniProt Protein Name    Melanoma antigen preferentially expressed in tumors
 UniProt Synonym Protein Names   
Opa-interacting protein 4; OIP-4; Preferentially expressed antigen of melanoma
 Protein Family    Melanoma antigen
 UniProt Gene Name    PRAME [Similar Products]
 UniProt Synonym Gene Names    MAPE; OIP4; OIP-4 [Similar Products]
 UniProt Entry Name    PRAME_HUMAN
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 NCBI Summary for PRAME    This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]
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 UniProt Comments for PRAME    PRAME: Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. Prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis. Belongs to the PRAME family.

Protein type: Cancer Testis Antigen (CTA); Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 22q11.22

Cellular Component: nucleus; plasma membrane

Molecular Function: protein binding; retinoic acid receptor binding

Biological Process: apoptosis; cell differentiation; negative regulation of apoptosis; negative regulation of cell differentiation; negative regulation of retinoic acid receptor signaling pathway; negative regulation of transcription, DNA-dependent; positive regulation of cell proliferation; regulation of growth; transcription, DNA-dependent
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 Research Articles on PRAME    1. PRAME immunoreactivity in myeloid leukemia (ML) of Down syndrome (DS) is largely due to the non-blast components, while PRAME immunoreactivity in blasts of Transient abnormal myelopoiesis (TAM) is not restricted to cases that progress to ML of DS.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PRAME elisa kitDiseases associated with PRAME elisa kit
 Reference Product  PubMed Publications
 CUL2 elisa kit  >2 publications with PRAME and CUL2
 OSGEP elisa kit  >1 publications with PRAME and OSGEP
 Disease Name  Pubmed Publications
 Melanoma Antibodies  >92 publications with PRAME and Melanoma
 Leukemia Antibodies  >90 publications with PRAME and Leukemia
 Leukemia, Myelogenous, Chronic, BCR-ABL Positive Antibodies  >18 publications with PRAME and Leukemia, Myelogenous, Chronic, BCR-ABL Positive
 Sarcoma Antibodies  >12 publications with PRAME and Sarcoma
 Carcinoma, Renal Cell Antibodies  >8 publications with PRAME and Carcinoma, Renal Cell
 Multiple Myeloma Antibodies  >8 publications with PRAME and Multiple Myeloma
 Necrosis Antibodies  >6 publications with PRAME and Necrosis
 Breast Neoplasms Antibodies  >5 publications with PRAME and Breast Neoplasms
 Carcinoma, Non-Small-Cell Lung Antibodies  >4 publications with PRAME and Carcinoma, Non-Small-Cell Lung
 Head and Neck Neoplasms Antibodies  >4 publications with PRAME and Head and Neck Neoplasms
Organs/Tissues associated with PRAME elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >75 publications with PRAME and Blood
 Testis Antibodies  >47 publications with PRAME and Testis
 Bone Marrow Antibodies  >32 publications with PRAME and Bone Marrow
 Lung Antibodies  >12 publications with PRAME and Lung
 Skin Antibodies  >10 publications with PRAME and Skin
 Kidney Antibodies  >8 publications with PRAME and Kidney
 Eye Antibodies  >8 publications with PRAME and Eye
 Brain Antibodies  >8 publications with PRAME and Brain
 Lymph Antibodies  >7 publications with PRAME and Lymph
 Prostate Antibodies  >5 publications with PRAME and Prostate
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