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C-ERC elisa kit :: Human Mesothelin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS764860
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $300 +1 FREE 8GB USB
96-Strip-Wells  /  $415 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Mesothelin (C-ERC), ELISA Kit

★Popular Item★
 Also Known As   

Human Mesothelin ELISA Kit

 Product Synonym Names    C-ERC,Mesothelin
 Product Gene Name   

C-ERC elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS764860 COA    COA PDF
 OMIM    601051
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of C-ERC. No significant cross-reactivity or interference between C-ERC and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    62.5-4000pg/ml
 Sensitivity    <37.5pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CERC were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CERC were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of C-ERC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for C-ERC purchase    MBS764860 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Mesothelin (C-ERC) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 62.5-4000pg/ml in biological research samples containing C-ERC, with an estimated sensitivity of <37.5pg/ml. The ELISA analytical biochemical technique of the MBS764860 kit is based on C-ERC antibody-C-ERC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect C-ERC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, C-ERC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for C-ERC elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- C-ERC antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- C-ERC antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the C-ERC amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of C-ERC can be calculated.
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 Typical Standard Curve/Testing Data of C-ERC elisa kit    C-ERC elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS764860. Click to request current manual
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NCBI/Uniprot data below describe general gene information for C-ERC. It may not necessarily be applicable to this product.
 NCBI GI #    293651530
 NCBI GeneID    10232
 NCBI Accession #    NP_001170826.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001177355.2 [Other Products]
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 UniProt Secondary Accession #    Q14859; Q4VQD5; Q96GR6; Q96KJ5; Q9BR17; Q9BTR2; Q9UCB2; Q9UK57; D3DU65 [Other Products]
 UniProt Related Accession #    Q13421 [Other Products]
 Molecular Weight    67,940 Da
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 NCBI Official Full Name    mesothelin isoform 1 preproprotein
 NCBI Official Synonym Full Names    mesothelin
 NCBI Official Symbol    MSLN [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    mesothelin
 UniProt Protein Name    Mesothelin
 UniProt Synonym Protein Names   
CAK1 antigen; Pre-pro-megakaryocyte-potentiating factor
 Protein Family    Mesothelin
 UniProt Gene Name    MSLN [Similar Products]
 UniProt Synonym Gene Names    MPF; MPF [Similar Products]
 UniProt Entry Name    MSLN_HUMAN
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 NCBI Summary for C-ERC    This gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010]
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 UniProt Comments for C-ERC    MSLN: Membrane-anchored forms may play a role in cellular adhesion. Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer. Belongs to the mesothelin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: cell surface; extracellular space; Golgi apparatus; membrane; plasma membrane

Molecular Function: protein binding

Biological Process: cell adhesion; cell-matrix adhesion; pancreas development
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 Research Articles on C-ERC    1. findings replicate association between rs1057147 and soluble mesothelin related peptide(SMRP)levels; SMRP performance as diagnostic biomarker for malignant pleural mesothelioma improved by considering genotype rs1057147; this polymorphism most likely affects a binding site for miR-611
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with C-ERC elisa kitDiseases associated with C-ERC elisa kit
 Reference Product  PubMed Publications
 MUC16 elisa kit  >3 publications with C-ERC and MUC16
 WFDC2 elisa kit  >2 publications with C-ERC and WFDC2
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >23 publications with C-ERC and Carcinoma
 Pancreatic Neoplasms Antibodies  >21 publications with C-ERC and Pancreatic Neoplasms
 Mesothelioma Antibodies  >20 publications with C-ERC and Mesothelioma
 Neoplasms, Mesothelial Antibodies  >18 publications with C-ERC and Neoplasms, Mesothelial
 Adenocarcinoma Antibodies  >18 publications with C-ERC and Adenocarcinoma
 Ovarian Neoplasms Antibodies  >17 publications with C-ERC and Ovarian Neoplasms
 Lung Diseases Antibodies  >11 publications with C-ERC and Lung Diseases
 Lung Neoplasms Antibodies  >10 publications with C-ERC and Lung Neoplasms
 Carcinoma, Pancreatic Ductal Antibodies  >9 publications with C-ERC and Carcinoma, Pancreatic Ductal
 Neoplasms, Experimental Antibodies  >8 publications with C-ERC and Neoplasms, Experimental
Organs/Tissues associated with C-ERC elisa kit
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >15 publications with C-ERC and Lung
 Liver Antibodies  >6 publications with C-ERC and Liver
 Pancreas Antibodies  >5 publications with C-ERC and Pancreas
 Stomach Antibodies  >4 publications with C-ERC and Stomach
 Ovary Antibodies  >3 publications with C-ERC and Ovary
 Ascites Antibodies  >2 publications with C-ERC and Ascites
 Intestine Antibodies  >2 publications with C-ERC and Intestine
 Brain Antibodies  >1 publications with C-ERC and Brain
 Prostate Antibodies  >1 publications with C-ERC and Prostate
 Cervix Antibodies  >1 publications with C-ERC and Cervix
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