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MTR elisa kit :: Human Methionine synthase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS763612
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $300 +1 FREE 8GB USB
96-Strip-Wells  /  $415 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Methionine synthase (MTR), ELISA Kit

★Popular Item★
 Also Known As   

Human Methionine synthase ELISA Kit

 Product Synonym Names    MTR/Methionine synthase/5-methyltetrahydrofolate--homocysteine methyltransferase/Vitamin-B12 dependent methionine synthase/MS
 Product Gene Name   

MTR elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS763612 COA    COA PDF
 MBS763612 QC Report    QC Report PDF
 OMIM    156570
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of MTR. No significant cross-reactivity or interference between MTR and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates and other biological fluids
 Detection Range    0.625-40ng/ml
 Sensitivity    <0.375ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MTR were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MTR were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of MTR elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MTR purchase    MBS763612 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Methionine synthase (MTR) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.625-40ng/ml in biological research samples containing MTR, with an estimated sensitivity of <0.375ng/ml. The ELISA analytical biochemical technique of the MBS763612 kit is based on MTR antibody-MTR antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MTR antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MTR. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for MTR elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- MTR antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- MTR antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP- Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MTR amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MTR can be calculated.
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 Typical Testing Data/Standard Curve (for reference only) of MTR elisa kit    MTR elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS763612. Click to request current manual
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NCBI/Uniprot data below describe general gene information for MTR. It may not necessarily be applicable to this product.
 NCBI GI #    2842762
 NCBI GeneID    4548
 NCBI Accession #    Q99707.2 [Other Products]
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 UniProt Secondary Accession #    Q99713; Q99723; A1L4N8; A9Z1W4; B7ZLW7; B9EGF7 [Other Products]
 UniProt Related Accession #    Q99707 [Other Products]
 Molecular Weight    134,793 Da
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 NCBI Official Full Name    Methionine synthase
 NCBI Official Synonym Full Names    5-methyltetrahydrofolate-homocysteine methyltransferase
 NCBI Official Symbol    MTR [Similar Products]
 NCBI Official Synonym Symbols   
MS; HMAG; cblG
[Similar Products]
 NCBI Protein Information    methionine synthase
 UniProt Protein Name    Methionine synthase
 UniProt Synonym Protein Names   
5-methyltetrahydrofolate--homocysteine methyltransferase; Vitamin-B12 dependent methionine synthase; MS
 Protein Family    Methionine synthase
 UniProt Gene Name    MTR [Similar Products]
 UniProt Synonym Gene Names    MS [Similar Products]
 UniProt Entry Name    METH_HUMAN
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 NCBI Summary for MTR    This gene encodes the 5-methyltetrahydrofolate-homocysteine methyltransferase. This enzyme, also known as cobalamin-dependent methionine synthase, catalyzes the final step in methionine biosynthesis. Mutations in MTR have been identified as the underlying cause of methylcobalamin deficiency complementation group G. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]
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 UniProt Comments for MTR    MTR: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate. Defects in MTR are the cause of methylcobalamin deficiency type G (cblG); also known as homocystinuria-megaloblastic anemia complementation type G. It is an autosomal recessive inherited disease that causes mental retardation, macrocytic anemia, and homocystinuria. Mild deficiency in MS activity could be associated with mild hyperhomocysteinemia, a risk factor for cardiovascular disease and possibly neural tube defects. MS mutations could also be involved in tumorigenesis. Defects in MTR may be a cause of susceptibility to folate-sensitive neural tube defects (FS-NTD). The most common NTDs are open spina bifida (myelomeningocele) and anencephaly. Genetic defects in MTR may affect the risk of spina bifida via the maternal rather than the embryonic genotype. Belongs to the vitamin-B12 dependent methionine synthase family.

Protein type: Amino Acid Metabolism - cysteine and methionine; Cofactor and Vitamin Metabolism - one carbon pool by folate; EC 2.1.1.13; Methyltransferase

Chromosomal Location of Human Ortholog: 1q43

Cellular Component: cytoplasm; cytosol

Molecular Function: cobalamin binding; homocysteine S-methyltransferase activity; methionine synthase activity; protein binding; zinc ion binding

Biological Process: axon regeneration; cobalamin metabolic process; methionine biosynthetic process; methylation; nervous system development; pteridine and derivative metabolic process; response to axon injury; sulfur amino acid metabolic process; vitamin metabolic process; water-soluble vitamin metabolic process; xenobiotic metabolic process

Disease: Homocystinuria-megaloblastic Anemia, Cblg Complementation Type; Neural Tube Defects, Folate-sensitive
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 Research Articles on MTR    1. Methionine synthase (MTR) and methionine synthase reductase (MTRR) polymorphisms were significantly associated with the increased neural tube defects risk in a Chinese population.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with MTR elisa kitPathways associated with MTR elisa kit
 Reference Product  PubMed Publications
 MTHFR elisa kit  >295 publications with MTR and MTHFR
 MTRR elisa kit  >236 publications with MTR and MTRR
 CBS elisa kit  >84 publications with MTR and CBS
 MTHFD1 elisa kit  >48 publications with MTR and MTHFD1
 AHCY elisa kit  >4 publications with MTR and AHCY
 MAT2A elisa kit  >3 publications with MTR and MAT2A
 MMACHC elisa kit  >3 publications with MTR and MMACHC
 MAT1A elisa kit  >2 publications with MTR and MAT1A
 MMADHC elisa kit  >1 publications with MTR and MMADHC
 Products by Pathway  Pathway Diagram
 Biological Oxidations Pathway antibodies  Biological Oxidations Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Cobalamin (Cbl, Vitamin B12) Transport And Metabolism Pathway antibodies  Cobalamin (Cbl, Vitamin B12) Transport And Metabolism Pathway Diagram
 Cysteine And Methionine Metabolism Pathway antibodies  Cysteine And Methionine Metabolism Pathway Diagram
 Cysteine And Methionine Metabolism Pathway antibodies  Cysteine And Methionine Metabolism Pathway Diagram
 Defective MTR Causes Methylmalonic Aciduria And Homocystinuria Type CblG Pathway antibodies  Defective MTR Causes Methylmalonic Aciduria And Homocystinuria Type CblG Pathway Diagram
 Defective MTRR Causes Methylmalonic Aciduria And Homocystinuria Type CblE Pathway antibodies  Defective MTRR Causes Methylmalonic Aciduria And Homocystinuria Type CblE Pathway Diagram
 Defects In Cobalamin (B12) Metabolism Pathway antibodies  Defects In Cobalamin (B12) Metabolism Pathway Diagram
 Defects In Vitamin And Cofactor Metabolism Pathway antibodies  Defects In Vitamin And Cofactor Metabolism Pathway Diagram
Diseases associated with MTR elisa kitOrgans/Tissues associated with MTR elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >506 publications with MTR and Nervous System Diseases
 Demyelinating Diseases Antibodies  >261 publications with MTR and Demyelinating Diseases
 Brain Diseases Antibodies  >240 publications with MTR and Brain Diseases
 Central Nervous System Diseases Antibodies  >222 publications with MTR and Central Nervous System Diseases
 Atrophy Antibodies  >81 publications with MTR and Atrophy
 Gastrointestinal Diseases Antibodies  >60 publications with MTR and Gastrointestinal Diseases
 Disease Models, Animal Antibodies  >46 publications with MTR and Disease Models, Animal
 Edema Antibodies  >43 publications with MTR and Edema
 Cognition Disorders Antibodies  >42 publications with MTR and Cognition Disorders
 Lymphoma Antibodies  >41 publications with MTR and Lymphoma
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >571 publications with MTR and Brain
 Blood Antibodies  >230 publications with MTR and Blood
 Nerve Antibodies  >144 publications with MTR and Nerve
 Liver Antibodies  >64 publications with MTR and Liver
 Bone Antibodies  >62 publications with MTR and Bone
 Vascular Antibodies  >56 publications with MTR and Vascular
 Muscle Antibodies  >55 publications with MTR and Muscle
 Heart Antibodies  >40 publications with MTR and Heart
 Lung Antibodies  >32 publications with MTR and Lung
 Kidney Antibodies  >31 publications with MTR and Kidney
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