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MTX elisa kit :: Human Methotrexate ELISA Kit

Scan QR to view Datasheet Catalog #    MBS017030
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $435 +1 FREE 8GB USB
96-Strip-Wells  /  $600 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,650 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,205 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Methotrexate (MTX), ELISA Kit

 Also Known As   

Human Methotrexate ELISA Kit

 Product Gene Name   

MTX elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    600605
 3D Structure    ModBase 3D Structure for Q13505
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 Species Reactivity    Human
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 Specificity    This kit recognizes recombinant and natural Human MTX, no significant cross-reactivity or interference was observed.
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 Samples    Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
 Detection Range    0.25 ug/ml ~ 8 ug/ml.
 Sensitivity    0.1 ug/ml.
 Intended Uses    This ELISA kit is intended for laboratory in vitro research use only, not for drug, household or other use! The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm by a spectrophotometer. In order to measure the concentration of Human MTX in the sample, this ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Human MTX concentration. The concentration of Human MTX in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Intra-assay Precision    Intra-assay CV (%) are less than 15%.
 Inter-assay Precision    Inter-assay CV (%) are less than 15%.
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of MTX elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MTX purchase    MBS017030 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Methotrexate (MTX) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.25 ug/ml ~ 8 ug/ml in biological research samples containing MTX, with an estimated sensitivity of 0.1 ug/ml. The ELISA analytical biochemical technique of the MBS017030 kit is based on MTX antibody-MTX antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MTX antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MTX. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for MTX elisa kit

   Introduction: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample.
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 Typical Testing Data/Standard Curve (for reference only) of MTX elisa kit    MTX elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS017030. Click to request current manual
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NCBI/Uniprot data below describe general gene information for MTX. It may not necessarily be applicable to this product.
 NCBI GI #    597955330
 NCBI GeneID    4580
 NCBI Accession #    NP_002446.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_002455.4 [Other Products]
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 UniProt Primary Accession #    Q13505 [Other Products]
 UniProt Secondary Accession #    Q9BUU3; B1AVR9; B1AVS0; B2R9P4 [Other Products]
 UniProt Related Accession #    Q13505 [Other Products]
 Molecular Weight    51,477 Da
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 NCBI Official Full Name    metaxin-1 isoform 1
 NCBI Official Synonym Full Names    metaxin 1
 NCBI Official Symbol    MTX1 [Similar Products]
 NCBI Official Synonym Symbols   
MTX; MTXN
[Similar Products]
 NCBI Protein Information    metaxin-1; mitochondrial outer membrane import complex protein 1
 UniProt Protein Name    Metaxin-1
 UniProt Synonym Protein Names   
Mitochondrial outer membrane import complex protein 1
 UniProt Gene Name    MTX1 [Similar Products]
 UniProt Synonym Gene Names    MTX; MTXN [Similar Products]
 UniProt Entry Name    MTX1_HUMAN
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 UniProt Comments for MTX    MTX1: Involved in transport of proteins into the mitochondrion. Essential for embryonic development. Belongs to the metaxin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: mitochondrial outer membrane; integral to membrane

Biological Process: cellular protein metabolic process; protein targeting to mitochondrion
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 Research Articles on MTX    1. Results describe the association between the MTX1 and beta-glucocerebrosidase genes and its possible effect on Parkinson disease.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with MTX elisa kitPathways associated with MTX elisa kit
 Reference Product  PubMed Publications
 MTX2 elisa kit  >14 publications with MTX and MTX2
 TOMM22 elisa kit  >1 publications with MTX and TOMM22
 TOMM20 elisa kit  >1 publications with MTX and TOMM20
 Products by Pathway  Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Mitochondrial Protein Import Pathway antibodies  Mitochondrial Protein Import Pathway Diagram
Diseases associated with MTX elisa kitOrgans/Tissues associated with MTX elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >3 publications with MTX and Neoplasms
 Necrosis Antibodies  >3 publications with MTX and Necrosis
 Weight Gain Antibodies  >1 publications with MTX and Weight Gain
 Inflammation Antibodies  >1 publications with MTX and Inflammation
 Infertility, Female Antibodies  >1 publications with MTX and Infertility, Female
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >5 publications with MTX and Blood
 Liver Antibodies  >4 publications with MTX and Liver
 Brain Antibodies  >4 publications with MTX and Brain
 Muscle Antibodies  >3 publications with MTX and Muscle
 Bone Antibodies  >3 publications with MTX and Bone
 Embryonic Tissue Antibodies  >3 publications with MTX and Embryonic Tissue
 Eye Antibodies  >2 publications with MTX and Eye
 Nerve Antibodies  >2 publications with MTX and Nerve
 Heart Antibodies  >2 publications with MTX and Heart
 Mammary Gland Antibodies  >1 publications with MTX and Mammary Gland
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