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MFAP5 elisa kit :: Human Microfibrillar-associated protein 5 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS762226
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $280 +1 FREE 8GB USB
96-Strip-Wells  /  $375 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,015 +3 FREE 8GB USB
15x96-Strip-Wells  /  $4,285 +5 FREE 8GB USB
25x96-Strip-Wells  /  $6,715 +8 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Microfibrillar-associated protein 5 (MFAP5), ELISA Kit

★Popular Item★
 Also Known As   

Human Microfibrillar-associated protein 5 ELISA Kit

 Product Synonym Names    MAGP-2/MFAP5 (Microfibrillar-associated protein 5)/MP25/MAGP2/MFAP-5/Microfibril-associated glycoprotein 2/microfibrillar associated protein 5/microfibril-associated glycoprotein-2
 Product Gene Name   

MFAP5 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS762226 COA    COA PDF
 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of MFAP5. No significant cross-reactivity or interference between MFAP5 and analogues was observed.
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 Samples    Serum, plasma and other biological fluids.
 Assay Type    Sandwich ELISA, Double Antibody
 Detection Range    0.312-20ng/ml
 Sensitivity    0.188ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MFAP5 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MFAP5 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of MFAP5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MFAP5 purchase    MBS762226 is a ready-to-use microwell, strip plate Sandwich, Double Antibody ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Microfibrillar-associated protein 5 (MFAP5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312-20ng/ml in biological research samples containing MFAP5, with an estimated sensitivity of 0.188ng/ml. The ELISA analytical biochemical technique of the MBS762226 kit is based on MFAP5 antibody-MFAP5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MFAP5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MFAP5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for MFAP5 elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- MFAP5 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- MFAP5 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MFAP5 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MFAP5 can be calculated.
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 Typical Testing Data/Standard Curve (for reference only) of MFAP5 elisa kit    MFAP5 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS762226. Click to request current manual
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NCBI/Uniprot data below describe general gene information for MFAP5. It may not necessarily be applicable to this product.
 NCBI GI #    27805993
 NCBI GeneID    281908
 NCBI Accession #    NP_776811.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_174386.2 [Other Products]
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 UniProt Secondary Accession #    Q3ZC93 [Other Products]
 UniProt Related Accession #    Q28022 [Other Products]
 Molecular Weight    19,080 Da
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 NCBI Official Full Name    microfibrillar-associated protein 5
 NCBI Official Symbol    MFAP5 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    microfibrillar-associated protein 5
 UniProt Protein Name    Microfibrillar-associated protein 5
 UniProt Synonym Protein Names   
MP25; Microfibril-associated glycoprotein 2; MAGP-2
 Protein Family    Microfibrillar-associated protein
 UniProt Gene Name    MFAP5 [Similar Products]
 UniProt Synonym Gene Names    MAGP2; MFAP-5; MAGP-2 [Similar Products]
 UniProt Entry Name    MFAP5_BOVIN
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 UniProt Comments for MFAP5    May play a role in hematopoiesis. In the cardiovascular system, could regulate growth factors or participate in cell signaling in maintaining large vessel integrity (). Component of the elastin-associated microfibrils ().
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 Research Articles on MFAP5    1. results show MAGP-2 is covalently and periodically located along the fibrillin-containing microfibrils of the developing nuchal ligament, suggesting that it is an integral component of most if not all of the microfibrils in this elastic fiber-rich tissue
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with MFAP5 elisa kitPathways associated with MFAP5 elisa kit
 Reference Product  PubMed Publications
 MFAP2 elisa kit  >3 publications with MFAP5 and MFAP2
 FBN1 elisa kit  >2 publications with MFAP5 and FBN1
 FBLN2 elisa kit  >1 publications with MFAP5 and FBLN2
 Products by Pathway  Pathway Diagram
 Elastic Fibre Formation Pathway antibodies  Elastic Fibre Formation Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Molecules Associated With Elastic Fibres Pathway antibodies  Molecules Associated With Elastic Fibres Pathway Diagram
Diseases associated with MFAP5 elisa kitOrgans/Tissues associated with MFAP5 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >3 publications with MFAP5 and Inflammation
 Adenocarcinoma Antibodies  >2 publications with MFAP5 and Adenocarcinoma
 Cardiovascular Diseases Antibodies  >1 publications with MFAP5 and Cardiovascular Diseases
 Ovarian Diseases Antibodies  >1 publications with MFAP5 and Ovarian Diseases
 Ovarian Neoplasms Antibodies  >1 publications with MFAP5 and Ovarian Neoplasms
 Neoplasms, Experimental Antibodies  >1 publications with MFAP5 and Neoplasms, Experimental
 Fibrosis Antibodies  >1 publications with MFAP5 and Fibrosis
 Weight Loss Antibodies  >1 publications with MFAP5 and Weight Loss
 Infertility, Male Antibodies  >1 publications with MFAP5 and Infertility, Male
 Cell Transformation, Neoplastic Antibodies  >1 publications with MFAP5 and Cell Transformation, Neoplastic
 Organ/Tissue Name  Pubmed Publications
 Adipose Tissue Antibodies  >3 publications with MFAP5 and Adipose Tissue
 Muscle Antibodies  >2 publications with MFAP5 and Muscle
 Liver Antibodies  >1 publications with MFAP5 and Liver
 Uterus Antibodies  >1 publications with MFAP5 and Uterus
 Lung Antibodies  >1 publications with MFAP5 and Lung
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