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MAP2K6 elisa kit :: Human Mitogen Activated Protein Kinase Kinase 6 (MAP2K6) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS9304470 MAP2K6 elisa kit
Unit / Price
48-Strip-Wells  /  $435 +1 FREE 8GB USB
96-Strip-Wells  /  $600 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Mitogen Activated Protein Kinase Kinase 6 (MAP2K6), ELISA Kit

 Also Known As   

Human Mitogen Activated Protein Kinase Kinase 6 (MAP2K6) ELISA Kit

 Product Gene Name   

MAP2K6 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 Chromosome Location    Chromosome: 12; NC_007123.5 (37501584..37550815, complement). Location: chromosome: 12
 3D Structure    ModBase 3D Structure for Q9DGE0
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 Species Reactivity    Human
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 Specificity    No significant cross-reactivity or interference between this analyte and analogues is observed.
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 Assay Type    Sandwich
 Detection Range    0.625 ng/ml - 20 ng/ml.
 Sensitivity    The sensitivity of this kit is 0.1 ng/ml.
 Intra-assay Precision    Intra-assay CV (%) is less than 15%.
 Inter-assay Precision    Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean 100].
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Other Notes    Small volumes of MAP2K6 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MAP2K6 purchase    MBS9304470 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Mitogen Activated Protein Kinase Kinase 6 (MAP2K6) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.625 ng/ml - 20 ng/ml in biological research samples containing MAP2K6, with an estimated sensitivity of The sensitivity of this kit is 0.1 ng/ml. The ELISA analytical biochemical technique of the MBS9304470 kit is based on MAP2K6 antibody-MAP2K6 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MAP2K6 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MAP2K6. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for MAP2K6 elisa kit

   Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of MAP2K6 (hereafter termed "analyte") in undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
Sample Manual Insert of MBS9304470
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NCBI/Uniprot data below describe general gene information for MAP2K6. It may not necessarily be applicable to this product.
 NCBI GI #    182889926
 NCBI GeneID    65239
 NCBI Accession #    AAI65822.1 [Other Products]
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 UniProt Primary Accession #    Q9DGE0 [Other Products]
 UniProt Secondary Accession #    Q6IQW6 [Other Products]
 UniProt Related Accession #    Q9DGE0 [Other Products]
 Molecular Weight    40,594 Da
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 NCBI Official Full Name    Map2k6 protein
 NCBI Official Synonym Full Names    mitogen-activated protein kinase kinase 6
 NCBI Official Symbol    map2k6 [Similar Products]
 NCBI Official Synonym Symbols   
MKK3; zMKK3; map2k3
[Similar Products]
 NCBI Protein Information    dual specificity mitogen-activated protein kinase kinase 6; MAPKK 6; MAP kinase kinase 6; mitogen-activated protein kinase kinase 3
 UniProt Protein Name    Dual specificity mitogen-activated protein kinase kinase 6
 UniProt Synonym Protein Names   
Mitogen-activated protein kinase kinase 3
 Protein Family    Dual specificity mitogen-activated protein kinase kinase
 UniProt Gene Name    map2k6 [Similar Products]
 UniProt Synonym Gene Names    map2k3; MAP kinase kinase 6; MAPKK 6; zMKK3 [Similar Products]
 UniProt Entry Name    MP2K6_DANRE
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 UniProt Comments for MAP2K6    Function: Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinases p38 and plays an important role in the regulation of cellular responses to cytokines and all kinds of stresses. The p38 MAP kinase signal transduction pathway leads to direct activation of transcription factors. Phosphorylation by MAP2K6 asymmetrically activates p38 on one side of the blastodisc, an event which is necessary for blastomere cleavage. Ref.1

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation: Activated by dual phosphorylation on Ser-234 and Thr-238 in response to a variety of cellular stresses, including UV radiation, osmotic shock, hypoxia, inflammatory cytokines, interferon gamma (IFNG), and less often by growth factors. MAP2K6/MKK6 is activated by the majority of M3Ks

By similarity.

Subunit structure: Dimer

By similarity. Interacts (via its D domain) with its MAP kinase substrates

By similarity. Interacts (via its DVD domain) with MAP3Ks activators

By similarity.

Subcellular location: Nucleus

By similarity. Cytoplasm

By similarity. Cytoplasm › cytoskeleton

By similarity. Note: Binds to microtubules

By similarity.

Developmental stage: Expressed both maternally and zygotically. Zygotic expression continues throughout development. Ref.1

Domain: The DVD domain (residues 338-361) contains a conserved docking site and is found in the mammalian MAP kinase kinases (MAP2Ks). The DVD sites bind to their specific upstream MAP kinase kinase kinases (MAP3Ks) and are essential for activation

By similarity.The D domain (residues 30-46) contains a conserved docking site and is required for the binding to MAPK substrates

By similarity.

Post-translational modification: Weakly autophosphorylated

By similarity. Phosphorylated at Ser-234 and Thr-238 by the majority of M3Ks.

Sequence similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.Contains 1 protein kinase domain.

Caution: Was originally (Ref.1) thought to be a Map2k3 protein but sequence analysis shows closer similarity to the Map2k6 proteins.

Sequence caution: The sequence AAH71285.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with MAP2K6 elisa kitDiseases associated with MAP2K6 elisa kit
 Products by Pathway  Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 FGF Signaling Pathway antibodies  FGF Signaling Pathway Diagram
 GnRH Signaling Pathway antibodies  GnRH Signaling Pathway Diagram
 GnRH Signaling Pathway antibodies  GnRH Signaling Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Integrin-mediated Cell Adhesion Pathway antibodies  Integrin-mediated Cell Adhesion Pathway Diagram
 MAP Kinase Activation In TLR Cascade Pathway antibodies  MAP Kinase Activation In TLR Cascade Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >49 publications with MAP2K6 and Neoplasms
 Necrosis Antibodies  >34 publications with MAP2K6 and Necrosis
 Inflammation Antibodies  >16 publications with MAP2K6 and Inflammation
 Disease Models, Animal Antibodies  >12 publications with MAP2K6 and Disease Models, Animal
 Adenocarcinoma Antibodies  >9 publications with MAP2K6 and Adenocarcinoma
 Nervous System Diseases Antibodies  >9 publications with MAP2K6 and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >8 publications with MAP2K6 and Cardiovascular Diseases
 Breast Neoplasms Antibodies  >8 publications with MAP2K6 and Breast Neoplasms
 Brain Diseases Antibodies  >7 publications with MAP2K6 and Brain Diseases
 Liver Diseases Antibodies  >7 publications with MAP2K6 and Liver Diseases
Organs/Tissues associated with MAP2K6 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >30 publications with MAP2K6 and Muscle
 Bone Antibodies  >19 publications with MAP2K6 and Bone
 Brain Antibodies  >12 publications with MAP2K6 and Brain
 Kidney Antibodies  >11 publications with MAP2K6 and Kidney
 Heart Antibodies  >9 publications with MAP2K6 and Heart
 Reproductive System Antibodies  >2 publications with MAP2K6 and Reproductive System
 Gills Antibodies  >1 publications with MAP2K6 and Gills
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