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| Product Name
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Mixed Lymphocyte Reaction Blocking Factors (MLR Bf), ELISA Kit
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| Also Known As
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Human Mixed Lymphocyte Reaction Blocking Factors ELISA Kit
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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| Sample Manual Insert
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| Request for Current Manual Insert
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| Species Reactivity
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Human
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| Samples
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Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
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| Assay Type
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Competitive
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| Detection Range
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250-5000pg/mL
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| Sensitivity
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1.0pg/mL
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| Intended Uses
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This MLR-Bf ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human MLR-Bf. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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| Preparation and Storage
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Store all reagents at 2-8 degree C.
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| Sample Preparation
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We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
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| Product Note
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Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
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| Other Notes
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Small volumes of MLR Bf elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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| Searchable Terms for MLR Bf purchase
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MBS756357 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Mixed Lymphocyte Reaction Blocking Factors (MLR Bf) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 250-5000pg/mL in biological research samples containing MLR Bf, with an estimated sensitivity of 1.0pg/mL. The ELISA analytical biochemical technique of the MBS756357 kit is based on MLR Bf antibody-MLR Bf antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MLR Bf antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MLR Bf. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Principle of the Assay: MLR-Bf ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MLR-Bf antibody and an MLR-Bf-HRP conjugate. The assay sample and buffer are incubated together with MLR-Bf-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MLR-Bf concentration since MLR-Bf from samples and MLR-Bf-HRP conjugate compete for the anti-MLR-Bf antibody binding site. Since the number of sites is limited, as more sites are occupied by MLR-Bf from the sample, fewer sites are left to bind MLR-Bf-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MLR-Bf concentration in each sample is interpolated from this standard curve.
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| Typical Testing Data/Standard Curve (for reference only) of MLR Bf elisa kit
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| Sample Manual Insert of MBS756357. Click to request current manual
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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ELISA Kit 
