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MUC1 elisa kit :: Human Mucin 1 (MUC1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2021290 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $245 +1 FREE 8GB USB
48-Strip-Wells  /  $415 +1 FREE 8GB USB
96-Strip-Wells  /  $555 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,220 +2 FREE 8GB USB
10x96-Strip-Wells  /  $4,000 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Mucin 1 (MUC1), ELISA Kit

★Popular Item★
 Also Known As   

Mucin 1 (MUC1) ELISA Kit

 Product Synonym Names    CD227; EMA; H23AG; KL-6; PEM; PEMT; PUM; CA15-3; Cancer antigen 15-3; Carcinoma-associated mucin; Episialin; Peanut-reactive urinary mucin; Polymorphic epithelial mucin
 Product Gene Name   

MUC1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    113720
 3D Structure    ModBase 3D Structure for P15941
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Mucin 1 (MUC1).
No significant cross-reactivity or interference between Mucin 1 (MUC1) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
 Detection Range    0.625-40ng/mL
 Sensitivity    < 0.266ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Mucin 1 (MUC1) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Mucin 1 (MUC1) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
 Supply Chain Verification    Manufactured in an a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of MUC1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MUC1 purchase    MBS2021290 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Mucin 1 (MUC1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.625-40ng/mL in biological research samples containing MUC1, with an estimated sensitivity of < 0.266ng/mL. The ELISA analytical biochemical technique of the MBS2021290 kit is based on MUC1 antibody-MUC1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MUC1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MUC1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for MUC1 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mucin 1 (MUC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mucin 1 (MUC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mucin 1 (MUC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mucin 1 (MUC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for MUC1 elisa kit    Tumor immunity; Infection immunity; Pulmonology
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 Typical Testing Data/Standard Curve (for reference only) of MUC1 elisa kit    MUC1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2021290. Click to request current manual
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NCBI/Uniprot data below describe general gene information for MUC1. It may not necessarily be applicable to this product.
 NCBI GI #    65301117
 NCBI GeneID    4582
 NCBI Accession #    NP_002447.4 [Other Products]
 NCBI GenBank Nucleotide #    NM_002456.5 [Other Products]
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 UniProt Primary Accession #    P15941 [Other Products]
 UniProt Secondary Accession #    P13931; P15942; P17626; A5YRV1; A6ZID9; A6ZIE0; B1AVQ8; B1AVR0; B6ECA1; E7ESE5; E7EUG9 [Other Products]
 UniProt Related Accession #    P15941 [Other Products]
 Molecular Weight    28,297 Da
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 NCBI Official Full Name    mucin-1 isoform 1
 NCBI Official Synonym Full Names    mucin 1, cell surface associated
 NCBI Official Symbol    MUC1 [Similar Products]
 NCBI Official Synonym Symbols   
EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC
[Similar Products]
 NCBI Protein Information    mucin-1
 UniProt Protein Name    Mucin-1
 UniProt Synonym Protein Names   
Breast carcinoma-associated antigen DF3; Cancer antigen 15-3; CA 15-3
 Protein Family    Mucin
 UniProt Gene Name    MUC1 [Similar Products]
 UniProt Synonym Gene Names    PUM; MUC-1; CA 15-3; KL-6; PUM; PEM; EMA; MUC1-NT; MUC1-alpha; MUC1-beta [Similar Products]
 UniProt Entry Name    MUC1_HUMAN
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 NCBI Summary for MUC1    This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011]
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 UniProt Comments for MUC1    MUC1: a large cell surface glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. Plays a role in adhesion and cell-cell interactions, metastasis and signaling. May provide a protective layer on epithelial surfaces. Direct or indirect interaction with actin cytoskeleton. Its cytoplasmic tail (MUC1CT) is involved in several signaling pathways, including those involving Ras, beta-catenin, p120 catenin, p53 and estrogen receptor alpha. MUC1CT also forms complexes with transcription factors, and then translocates to the nucleus by an unknown mechanism, where it is believed to influence the transcription of their target genes. MUC1CT has also been proposed to localize to mitochondrial membranes under conditions of genotoxic stress, where it attenuates the apoptotic pathway in response and confers resistance to apoptosis-inducing drugs. Aberrantly glycosylated forms expressed in human epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Nine alternatively spliced isoforms have been described. Isoforms 5 and 9 are secreted. Isoform 7, expressed only in tumor cells, is a receptor and binds the secreted isoform 5. The binding induces the phosphorylation of the isoform 7, alters cellular morphology and initiates cell signaling. Can bind to GRB2 adapter protein.

Protein type: Motility/polarity/chemotaxis; Actin-binding; Membrane protein, integral; Nuclear receptor co-regulator; Cell adhesion; Tumor suppressor

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: extracellular space; cell surface; Golgi lumen; integral to plasma membrane; cytoplasm; nuclear chromatin; apical plasma membrane; vesicle

Molecular Function: protein binding; p53 binding; transcription cofactor activity

Biological Process: protein amino acid O-linked glycosylation; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; cellular protein metabolic process; regulation of transcription from RNA polymerase II promoter in response to stress; O-glycan processing; post-translational protein modification; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest

Disease: Medullary Cystic Kidney Disease 1
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 Research Articles on MUC1    1. These findings support a model in which targeting MUC1-C inhibits mutant KRAS signaling in Non-small cell lung cancer cells and thereby reverses the epithelial-mesenchymal transition phenotype and decreases self-renewal.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with MUC1 elisa kitDiseases associated with MUC1 elisa kit
 Products by Pathway  Pathway Diagram
 IL-7 Signaling Pathway antibodies  IL-7 Signaling Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 O-linked Glycosylation Pathway antibodies  O-linked Glycosylation Pathway Diagram
 O-linked Glycosylation Of Mucins Pathway antibodies  O-linked Glycosylation Of Mucins Pathway Diagram
 Post-translational Protein Modification Pathway antibodies  Post-translational Protein Modification Pathway Diagram
 Termination Of O-glycan Biosynthesis Pathway antibodies  Termination Of O-glycan Biosynthesis Pathway Diagram
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >1167 publications with MUC1 and Carcinoma
 Breast Neoplasms Antibodies  >605 publications with MUC1 and Breast Neoplasms
 Lung Diseases Antibodies  >447 publications with MUC1 and Lung Diseases
 Lung Neoplasms Antibodies  >201 publications with MUC1 and Lung Neoplasms
 Neoplasms, Experimental Antibodies  >174 publications with MUC1 and Neoplasms, Experimental
 Ovarian Neoplasms Antibodies  >170 publications with MUC1 and Ovarian Neoplasms
 Disease Models, Animal Antibodies  >164 publications with MUC1 and Disease Models, Animal
 Inflammation Antibodies  >116 publications with MUC1 and Inflammation
 Necrosis Antibodies  >112 publications with MUC1 and Necrosis
 Liver Neoplasms Antibodies  >108 publications with MUC1 and Liver Neoplasms
Organs/Tissues associated with MUC1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >520 publications with MUC1 and Lung
 Stomach Antibodies  >212 publications with MUC1 and Stomach
 Pancreas Antibodies  >204 publications with MUC1 and Pancreas
 Liver Antibodies  >183 publications with MUC1 and Liver
 Intestine Antibodies  >153 publications with MUC1 and Intestine
 Prostate Antibodies  >100 publications with MUC1 and Prostate
 Eye Antibodies  >98 publications with MUC1 and Eye
 Kidney Antibodies  >96 publications with MUC1 and Kidney
 Uterus Antibodies  >77 publications with MUC1 and Uterus
 Ovary Antibodies  >58 publications with MUC1 and Ovary
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