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MLCK elisa kit :: Human Myosin Light Chain Kinase (MLCK) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS262705 MLCK elisa kit
Unit / Price
48-Strip-Wells  /  $260 +1 FREE 8GB USB
96-Strip-Wells  /  $405 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $2,880 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Myosin Light Chain Kinase (MLCK), ELISA Kit

 Also Known As   

Human Myosin Light Chain Kinase (MLCK) ELISA Kit

 Product Gene Name   

MLCK elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Request for Current Manual Insert    Request Current Manual
 OMIM    gene 613780
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 Species Reactivity    Human
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of MLCK elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MLCK purchase    MBS262705 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Myosin Light Chain Kinase (MLCK) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MLCK. The ELISA analytical biochemical technique of the MBS262705 kit is based on MLCK antibody-MLCK antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MLCK antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MLCK. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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NCBI/Uniprot data below describe general gene information for MLCK. It may not necessarily be applicable to this product.
 NCBI GI #    7239696
 NCBI GeneID    4638
 NCBI Accession #    AAC18423.2 [Other Products]
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 UniProt Secondary Accession #    O95796; O95797; O95798; O95799; Q14844; Q16794; Q17S15; Q3ZCP9; Q5MY99; B4DUE3; D3DN97 [Other Products]
 UniProt Related Accession #    Q15746 [Other Products]
 Molecular Weight    110,076 Da
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 NCBI Official Full Name    myosin light chain kinase
 NCBI Official Synonym Full Names    myosin light chain kinase
 NCBI Official Symbol    MYLK [Similar Products]
 NCBI Official Synonym Symbols   
KRP; AAT7; MLCK; MLCK1; MYLK1; smMLCK; MLCK108; MLCK210; MSTP083
[Similar Products]
 NCBI Protein Information    myosin light chain kinase, smooth muscle; telokin; kinase-related protein; myosin, light polypeptide kinase; smooth muscle myosin light chain kinase
 UniProt Protein Name    Myosin light chain kinase, smooth muscle
 UniProt Synonym Protein Names   
Kinase-related protein; KRP; TelokinCleaved into the following chain:Myosin light chain kinase, smooth muscle, deglutamylated form
 UniProt Gene Name    MYLK [Similar Products]
 UniProt Synonym Gene Names    MLCK; MLCK1; MYLK1; MLCK; smMLCK; KRP [Similar Products]
 UniProt Entry Name    MYLK_HUMAN
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 NCBI Summary for MLCK    This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts. [provided by RefSeq, Jul 2008]
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 UniProt Comments for MLCK    smMLCK: Calcium/calmodulin-dependent myosin light chain kinase implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activity. Phosphorylates PTK2B/PYK2 and myosin light-chains. Involved in the inflammatory response (e.g. apoptosis, vascular permeability, leukocyte diapedesis), cell motility and morphology, airway hyperreactivity and other activities relevant to asthma. Required for tonic airway smooth muscle contraction that is necessary for physiological and asthmatic airway resistance. Necessary for gastrointestinal motility. Implicated in the regulation of endothelial as well as vascular permeability, probably via the regulation of cytoskeletal rearrangements. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. Critical participant in signaling sequences that result in fibroblast apoptosis. Plays a role in the regulation of epithelial cell survival. Required for epithelial wound healing, especially during actomyosin ring contraction during purse-string wound closure. Mediates RhoA- dependent membrane blebbing. Triggers TRPC5 channel activity in a calcium-dependent signaling, by inducing its subcellular localization at the plasma membrane. Promotes cell migration (including tumor cells) and tumor metastasis. PTK2B/PYK2 activation by phosphorylation mediates ITGB2 activation and is thus essential to trigger neutrophil transmigration during acute lung injury (ALI). May regulate optic nerve head astrocyte migration. Probably involved in mitotic cytoskeletal regulation. Regulates tight junction probably by modulating ZO-1 exchange in the perijunctional actomyosin ring. Mediates burn-induced microvascular barrier injury; triggers endothelial contraction in the development of microvascular hyperpermeability by phosphorylating MLC. Essential for intestinal barrier dysfunction. Mediates Giardia spp.-mediated reduced epithelial barrier function during giardiasis intestinal infection via reorganization of cytoskeletal F-actin and tight junctional ZO-1. Necessary for hypotonicity-induced Ca(2+) entry and subsequent activation of volume-sensitive organic osmolyte/anion channels (VSOAC) in cervical cancer cells. Responsible for high proliferative ability of breast cancer cells through anti-apoptosis. Defects in MYLK are the cause of familial aortic aneurysm thoracic type 7 (AAT7). AAT7 is a disease characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. It is primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.18; Kinase, protein; Protein kinase, CAMK; CAMK group; MLCK family

Chromosomal Location of Human Ortholog: 3q21

Cellular Component: lamellipodium; cytoplasm; stress fiber; intercellular junction; cytosol; cleavage furrow

Molecular Function: calmodulin binding; protein binding; calmodulin-dependent protein kinase activity; metal ion binding; actin binding; ATP binding; myosin light chain kinase activity

Biological Process: tonic smooth muscle contraction; smooth muscle contraction; muscle contraction; actin filament organization; bleb formation; positive regulation of calcium ion transport; protein amino acid phosphorylation; positive regulation of cell migration

Disease: Aortic Aneurysm, Familial Thoracic 7
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 Research Articles on MLCK    1. Results suggest that Aurora B, but not Rho/MLCK (myosin-light-chain kinase) signaling, is essential for the localization of 2P-MRLC (myosin regulatory light chains) to the midzone in dividing HeLa cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with MLCK elisa kitDiseases associated with MLCK elisa kit
 Products by Pathway  Pathway Diagram
 Aurora B Signaling Pathway antibodies  Aurora B Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Endothelin Pathway antibodies  Endothelin Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Gastric Acid Secretion Pathway antibodies  Gastric Acid Secretion Pathway Diagram
 Gastric Acid Secretion Pathway antibodies  Gastric Acid Secretion Pathway Diagram
 MicroRNAs In Cardiomyocyte Hypertrophy Pathway antibodies  MicroRNAs In Cardiomyocyte Hypertrophy Pathway Diagram
 Disease Name  Pubmed Publications
 Acute Lung Injury Antibodies  >9 publications with MLCK and Acute Lung Injury
 Inflammation Antibodies  >7 publications with MLCK and Inflammation
 Vascular Diseases Antibodies  >7 publications with MLCK and Vascular Diseases
 Neoplasms Antibodies  >6 publications with MLCK and Neoplasms
 Hypersensitivity Antibodies  >6 publications with MLCK and Hypersensitivity
 Glaucoma Antibodies  >2 publications with MLCK and Glaucoma
 Pneumonia Antibodies  >2 publications with MLCK and Pneumonia
 Gastrointestinal Diseases Antibodies  >2 publications with MLCK and Gastrointestinal Diseases
 Disease Models, Animal Antibodies  >2 publications with MLCK and Disease Models, Animal
 Hypertension Antibodies  >2 publications with MLCK and Hypertension
Organs/Tissues associated with MLCK elisa kit
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >24 publications with MLCK and Muscle
 Vascular Antibodies  >14 publications with MLCK and Vascular
 Lung Antibodies  >13 publications with MLCK and Lung
 Blood Antibodies  >7 publications with MLCK and Blood
 Eye Antibodies  >3 publications with MLCK and Eye
 Stomach Antibodies  >2 publications with MLCK and Stomach
 Intestine Antibodies  >2 publications with MLCK and Intestine
 Nerve Antibodies  >2 publications with MLCK and Nerve
 Brain Antibodies  >2 publications with MLCK and Brain
 Uterus Antibodies  >2 publications with MLCK and Uterus
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