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NSF elisa kit :: Human N-Ethylmaleimide Sensitive Factor (NSF) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2020015 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $270 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $665 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,715 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,895 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

N-Ethylmaleimide Sensitive Factor (NSF), ELISA Kit

★Popular Item★
 Also Known As   

N-Ethylmaleimide Sensitive Factor (NSF) ELISA Kit

 Product Synonym Names    SKD2; N-Ethylmaleimide Sensitive Fusion Protein; Vesicle-fusing ATPase; Vesicular-fusion protein NSF
 Product Gene Name   

NSF elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    601633
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of N-Ethylmaleimide Sensitive Factor (NSF).
No significant cross-reactivity or interference between N-Ethylmaleimide Sensitive Factor (NSF) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Tissue homogenates, Cell lysates and Other Biological Fluids
 Detection Range    0.312-20ng/mL
 Sensitivity    < 0.117ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level N-Ethylmaleimide Sensitive Factor (NSF) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level N-Ethylmaleimide Sensitive Factor (NSF) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NSF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NSF purchase    MBS2020015 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the N-Ethylmaleimide Sensitive Factor (NSF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312-20ng/mL in biological research samples containing NSF, with an estimated sensitivity of < 0.117ng/mL. The ELISA analytical biochemical technique of the MBS2020015 kit is based on NSF antibody-NSF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NSF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NSF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates, Cell lysates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for NSF elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Ethylmaleimide Sensitive Factor (NSF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to N-Ethylmaleimide Sensitive Factor (NSF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-Ethylmaleimide Sensitive Factor (NSF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of N-Ethylmaleimide Sensitive Factor (NSF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for NSF elisa kit    Neuroscience
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 Typical Testing Data/Standard Curve (for reference only) of NSF elisa kit    NSF elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2020015. Click to request current manual
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NCBI/Uniprot data below describe general gene information for NSF. It may not necessarily be applicable to this product.
 NCBI GI #    467977
 NCBI GeneID    4905
 NCBI Accession #    AAA17411.1 [Other Products]
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 UniProt Secondary Accession #    Q8N6D7; Q9UKZ2; A8K2D9; B4DFA2 [Other Products]
 UniProt Related Accession #    P46459 [Other Products]
 Molecular Weight    71,584 Da
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 NCBI Official Full Name    N-ethylmaleimide-sensitive factor, partial
 NCBI Official Synonym Full Names    N-ethylmaleimide-sensitive factor
 NCBI Official Symbol    NSF [Similar Products]
 NCBI Official Synonym Symbols   
SKD2
[Similar Products]
 NCBI Protein Information    vesicle-fusing ATPase
 UniProt Protein Name    Vesicle-fusing ATPase
 UniProt Synonym Protein Names   
N-ethylmaleimide-sensitive fusion protein; NEM-sensitive fusion protein; Vesicular-fusion protein NSF
 UniProt Gene Name    NSF [Similar Products]
 UniProt Synonym Gene Names    NEM-sensitive fusion protein [Similar Products]
 UniProt Entry Name    NSF_HUMAN
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 UniProt Comments for NSF    NSF: required for intracellular membrane fusion and vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Functions as a SNAP receptor (SNARE) chaperone which binds, through soluble NSF attachment proteins (SNAPs), to SNARE complexes and utilizes the energy of ATP hydrolysis to disassemble them thus facilitating SNARE recycling. While this is a major function of NSF, it does seem to interact with other proteins, such as the AMPA receptor subunit, GluR2, and beta2-AR and is thought to affect their trafficking patterns.

Protein type: Autophagy; Vesicle; EC 3.6.4.6; Adaptor/scaffold; Protease

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: Golgi stack; lysosomal membrane; cytoplasm; postsynaptic density; plasma membrane; dendritic shaft; cytosol

Molecular Function: protein binding; metal ion binding; ATPase activity; syntaxin-1 binding; protein complex binding; ATPase activity, coupled; protein kinase binding; Rab GTPase binding; ATP binding; PDZ domain binding

Biological Process: regulation of exocytosis; vesicle-mediated transport; synaptic transmission; intra-Golgi vesicle-mediated transport; exocytosis; metabolic process; Golgi to plasma membrane protein transport; Golgi vesicle docking; positive regulation of receptor recycling; potassium ion transport; plasma membrane fusion
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 Research Articles on NSF    1. a processive helicase-like mechanism for NSF in which approximately 1 residue is unwound for every hydrolyzed ATP molecule.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with NSF elisa kitPathways associated with NSF elisa kit
 Reference Product  PubMed Publications
 STX1A elisa kit  >25 publications with NSF and STX1A
 PICK1 elisa kit  >19 publications with NSF and PICK1
 GOSR1 elisa kit  >4 publications with NSF and GOSR1
 NAPA elisa kit  >2 publications with NSF and NAPA
 VAPA elisa kit  >1 publications with NSF and VAPA
 GABARAPL1 elisa kit  >1 publications with NSF and GABARAPL1
 GRIA2 elisa kit  >1 publications with NSF and GRIA2
 GRIP2 elisa kit  >1 publications with NSF and GRIP2
 NAPG elisa kit  >1 publications with NSF and NAPG
 Products by Pathway  Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 GABAergic Synapse Pathway antibodies  GABAergic Synapse Pathway Diagram
 GABAergic Synapse Pathway antibodies  GABAergic Synapse Pathway Diagram
 Glutamate Binding, Activation Of AMPA Receptors And Synaptic Plasticity Pathway antibodies  Glutamate Binding, Activation Of AMPA Receptors And Synaptic Plasticity Pathway Diagram
 Neuronal System Pathway antibodies  Neuronal System Pathway Diagram
 Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell Pathway antibodies  Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell Pathway Diagram
 Synaptic Vesicle Pathway antibodies  Synaptic Vesicle Pathway Diagram
 Synaptic Vesicle Cycle Pathway antibodies  Synaptic Vesicle Cycle Pathway Diagram
 Synaptic Vesicle Cycle Pathway antibodies  Synaptic Vesicle Cycle Pathway Diagram
 Trafficking Of AMPA Receptors Pathway antibodies  Trafficking Of AMPA Receptors Pathway Diagram
Diseases associated with NSF elisa kitOrgans/Tissues associated with NSF elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >1667 publications with NSF and Nervous System Diseases
 Brain Diseases Antibodies  >1025 publications with NSF and Brain Diseases
 Cardiovascular Diseases Antibodies  >508 publications with NSF and Cardiovascular Diseases
 Fibrosis Antibodies  >368 publications with NSF and Fibrosis
 Kidney Diseases Antibodies  >247 publications with NSF and Kidney Diseases
 Brain Injuries Antibodies  >116 publications with NSF and Brain Injuries
 Disease Models, Animal Antibodies  >102 publications with NSF and Disease Models, Animal
 Seizures Antibodies  >101 publications with NSF and Seizures
 Neurodegenerative Diseases Antibodies  >75 publications with NSF and Neurodegenerative Diseases
 Inflammation Antibodies  >71 publications with NSF and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >1114 publications with NSF and Brain
 Bone Antibodies  >737 publications with NSF and Bone
 Nerve Antibodies  >517 publications with NSF and Nerve
 Blood Antibodies  >476 publications with NSF and Blood
 Vascular Antibodies  >393 publications with NSF and Vascular
 Kidney Antibodies  >276 publications with NSF and Kidney
 Skin Antibodies  >251 publications with NSF and Skin
 Muscle Antibodies  >168 publications with NSF and Muscle
 Heart Antibodies  >97 publications with NSF and Heart
 Liver Antibodies  >90 publications with NSF and Liver
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