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NGLY1 elisa kit :: Human N-glycanase 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS916030
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

N-glycanase 1 (NGLY1), ELISA Kit

 Also Known As   

Human Peptide-N, NGLY1 ELISA Kit

 Product Synonym Names    Human Peptide-N (NGLY1) ELISA kit; FLJ11005; FLJ12409; PNG1; PNGase; peptide-N (4) - (N-acetyl-beta-glucosaminyl) asparagine amidase; N-glycanase 1
 Product Gene Name   

NGLY1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS916030 Testing Data    Testing Data PDF
 OMIM    gene 615273
 3D Structure    ModBase 3D Structure for Q96IV0
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human NGLY1. No significant cross-reactivity or interference between human NGLY1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    37.5 pg/ml -2400 pg/ml.
 Sensitivity    The minimum detectable dose of human NGLY1 is typically less than 9.38 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NGLY1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NGLY1 purchase    MBS916030 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the N-glycanase 1 (NGLY1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 37.5 pg/ml -2400 pg/ml in biological research samples containing NGLY1, with an estimated sensitivity of The minimum detectable dose of human NGLY1 is typically less than 9.38 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS916030 kit is based on NGLY1 antibody-NGLY1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NGLY1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NGLY1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for NGLY1 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NGLY1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NGLY1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NGLY1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NGLY1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of NGLY1 elisa kit    NGLY1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS916030. Click to request current manual
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NCBI/Uniprot data below describe general gene information for NGLY1. It may not necessarily be applicable to this product.
 NCBI GI #    223941798
 NCBI GeneID    55768
 NCBI Accession #    NP_001138765.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001145293.1 [Other Products]
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 UniProt Primary Accession #    Q96IV0 [Other Products]
 UniProt Secondary Accession #    Q59FB1; Q6PJD8; Q9BVR8; Q9NR70; B4DJE9 [Other Products]
 UniProt Related Accession #    Q96IV0 [Other Products]
 Molecular Weight    74,390 Da
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 NCBI Official Full Name    peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase isoform 2
 NCBI Official Synonym Full Names    N-glycanase 1
 NCBI Official Symbol    NGLY1 [Similar Products]
 NCBI Official Synonym Symbols   
PNG1; CDG1V; PNGase
[Similar Products]
 NCBI Protein Information    peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase; peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase; hPNGase; peptide:N-glycanase
 UniProt Protein Name    Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase
 UniProt Synonym Protein Names   
N-glycanase 1; Peptide:N-glycanase
 UniProt Gene Name    NGLY1 [Similar Products]
 UniProt Synonym Gene Names    PNG1; PNGase; hPNGase [Similar Products]
 UniProt Entry Name    NGLY1_HUMAN
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 NCBI Summary for NGLY1    This gene encodes an enzyme that catalyzes hydrolysis of an N(4)-(acetyl-beta-D-glucosaminyl) asparagine residue to N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue. The encoded enzyme may play a role in the proteasome-mediated degradation of misfolded glycoproteins. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2009]
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 UniProt Comments for NGLY1    NGLY1: Specifically deglycosylates the denatured form of N- linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl- glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high- mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins. Belongs to the transglutaminase-like superfamily. PNGase family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; EC 3.5.1.52

Chromosomal Location of Human Ortholog: 3p24.2

Cellular Component: cytoplasm

Molecular Function: protein binding; peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity; metal ion binding

Biological Process: glycoprotein catabolic process

Disease: Congenital Disorder Of Deglycosylation
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 Research Articles on NGLY1    1. PNGase-PUB serves not only as p97-binding module but also as a possible activator of HR23 in endoplasmic reticulum-associated degradation mechanisms.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with NGLY1 elisa kitDiseases associated with NGLY1 elisa kit
 Products by Pathway  Pathway Diagram
 Protein Processing In Endoplasmic Reticulum Pathway antibodies  Protein Processing In Endoplasmic Reticulum Pathway Diagram
 Protein Processing In Endoplasmic Reticulum Pathway antibodies  Protein Processing In Endoplasmic Reticulum Pathway Diagram
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >1 publications with NGLY1 and Nervous System Diseases
 Movement Disorders Antibodies  >1 publications with NGLY1 and Movement Disorders
 Brain Diseases Antibodies  >1 publications with NGLY1 and Brain Diseases
Organs/Tissues associated with NGLY1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >1 publications with NGLY1 and Testis
 Placenta Antibodies  >1 publications with NGLY1 and Placenta
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