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SGSH elisa kit :: Human N-sulfoglucosamine sulfohydrolase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS932877
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
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 Product Name   

N-sulfoglucosamine sulfohydrolase (SGSH), ELISA Kit

★Popular Item★
 Also Known As   

Human N-sulphoglucosamine sulphohydrolase, SGSH ELISA Kit

 Product Synonym Names    Human N-sulphoglucosamine sulphohydrolase (SGSH) ELISA kit; HSS; MPS3A; SFMD; heparan sulfate sulfatase; sulfamidase; sulfoglucosamine sulfamidase; N-sulfoglucosamine sulfohydrolase
 Product Gene Name   

SGSH elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS932877 Testing Data    Testing Data PDF
 OMIM    605270
 3D Structure    ModBase 3D Structure for P51688
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human SGSH. No significant cross-reactivity or interference between human SGSH and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, cell lysates
 Assay Type    Quantitative Sandwich
 Detection Range    31.25 pg/ml -2000 pg/ml
 Sensitivity    7.81 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of SGSH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for SGSH purchase    MBS932877 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the N-sulfoglucosamine sulfohydrolase (SGSH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25 pg/ml -2000 pg/ml in biological research samples containing SGSH, with an estimated sensitivity of 7.81 pg/ml. The ELISA analytical biochemical technique of the MBS932877 kit is based on SGSH antibody-SGSH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SGSH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SGSH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for SGSH elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SGSH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SGSH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SGSH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SGSH bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of SGSH elisa kit    SGSH elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS932877. Click to request current manual
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NCBI/Uniprot data below describe general gene information for SGSH. It may not necessarily be applicable to this product.
 NCBI GI #    4506919
 NCBI GeneID    6448
 NCBI Accession #    NP_000190.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000199.3 [Other Products]
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 UniProt Primary Accession #    P51688 [Other Products]
 UniProt Secondary Accession #    A8K5E2 [Other Products]
 UniProt Related Accession #    P51688 [Other Products]
 Molecular Weight    56,695 Da
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 NCBI Official Full Name    N-sulphoglucosamine sulphohydrolase
 NCBI Official Synonym Full Names    N-sulfoglucosamine sulfohydrolase
 NCBI Official Symbol    SGSH [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    N-sulphoglucosamine sulphohydrolase; sulphamidase; heparan sulfate sulfatase; sulfoglucosamine sulfamidase; mucopolysaccharidosis type IIIA
 UniProt Protein Name    N-sulphoglucosamine sulphohydrolase
 UniProt Synonym Protein Names   
Sulfoglucosamine sulfamidase; Sulphamidase
 Protein Family    N-sulphoglucosamine sulphohydrolase
 UniProt Gene Name    SGSH [Similar Products]
 UniProt Synonym Gene Names    HSS [Similar Products]
 UniProt Entry Name    SPHM_HUMAN
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 NCBI Summary for SGSH    This gene encodes one of several enzymes involved in the lysosomal degradation of heparan sulfate. Mutations in this gene are associated with Sanfilippo syndrome A, one type of the lysosomal storage disease mucopolysaccaridosis III, which results from impaired degradation of heparan sulfate. Transcripts of varying sizes have been reported but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
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 UniProt Comments for SGSH    SGSH: Defects in SGSH are the cause of mucopolysaccharidosis type 3A (MPS3A); also known as Sanfilippo syndrome A. MPS3A is a severe form of mucopolysaccharidosis type 3, an autosomal recessive lysosomal storage disease due to impaired degradation of heparan sulfate. MPS3 is characterized by severe central nervous system degeneration, but only mild somatic disease. Onset of clinical features usually occurs between 2 and 6 years; severe neurologic degeneration occurs in most patients between 6 and 10 years of age, and death occurs typically during the second or third decade of life. MPS3A is characterized by earlier onset, rapid progression of symptoms and shorter survival. Belongs to the sulfatase family.

Protein type: Glycan Metabolism - glycosaminoglycan degradation; Hydrolase; EC

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: lysosomal lumen

Molecular Function: N-sulfoglucosamine sulfohydrolase activity; metal ion binding; sulfuric ester hydrolase activity; catalytic activity

Biological Process: proteoglycan metabolic process; glycosaminoglycan catabolic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; pathogenesis

Disease: Mucopolysaccharidosis, Type Iiia
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 Research Articles on SGSH    1. Pre-symptomatic treatment of progressive neurodegenerative disease (mucopolysaccharidosis type IIIA) via intra-cerebrospinal fluid injection of recombinant human SGSH mediates highly significant reductions in neuropathology in a canine model.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with SGSH elisa kitPathways associated with SGSH elisa kit
 Reference Product  PubMed Publications
 NAGLU elisa kit  >5 publications with SGSH and NAGLU
 IDUA elisa kit  >3 publications with SGSH and IDUA
 SUMF1 elisa kit  >2 publications with SGSH and SUMF1
 GNS elisa kit  >1 publications with SGSH and GNS
 HGSNAT elisa kit  >1 publications with SGSH and HGSNAT
 Products by Pathway  Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Glycogen Storage Diseases Pathway antibodies  Glycogen Storage Diseases Pathway Diagram
 Glycosaminoglycan Degradation Pathway antibodies  Glycosaminoglycan Degradation Pathway Diagram
 Glycosaminoglycan Degradation Pathway antibodies  Glycosaminoglycan Degradation Pathway Diagram
 Glycosaminoglycan Metabolism Pathway antibodies  Glycosaminoglycan Metabolism Pathway Diagram
 HS-GAG Degradation Pathway antibodies  HS-GAG Degradation Pathway Diagram
 Heparan Sulfate Degradation Pathway antibodies  Heparan Sulfate Degradation Pathway Diagram
 Heparan Sulfate Degradation Pathway antibodies  Heparan Sulfate Degradation Pathway Diagram
 Heparan Sulfate/heparin (HS-GAG) Metabolism Pathway antibodies  Heparan Sulfate/heparin (HS-GAG) Metabolism Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
Diseases associated with SGSH elisa kitOrgans/Tissues associated with SGSH elisa kit
 Disease Name  Pubmed Publications
 Mucopolysaccharidosis III Antibodies  >20 publications with SGSH and Mucopolysaccharidosis III
 Death Antibodies  >6 publications with SGSH and Death
 Nervous System Diseases Antibodies  >5 publications with SGSH and Nervous System Diseases
 Splenomegaly Antibodies  >1 publications with SGSH and Splenomegaly
 Hypertrophy Antibodies  >1 publications with SGSH and Hypertrophy
 Body Weight Changes Antibodies  >1 publications with SGSH and Body Weight Changes
 Immune System Diseases Antibodies  >1 publications with SGSH and Immune System Diseases
 Atrophy Antibodies  >1 publications with SGSH and Atrophy
 Inflammation Antibodies  >1 publications with SGSH and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >9 publications with SGSH and Brain
 Blood Antibodies  >2 publications with SGSH and Blood
 Liver Antibodies  >2 publications with SGSH and Liver
 Vascular Antibodies  >1 publications with SGSH and Vascular
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