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NELL1 elisa kit :: Human NEL-like 1 (chicken) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS900586
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

NEL-like 1 (chicken) (NELL1), ELISA Kit

★Popular Item★
 Also Known As   

Human Protein kinase C-binding protein NELL1, NELL1 ELISA Kit

 Product Synonym Names    Human Protein kinase C-binding protein NELL1 (NELL1) ELISA kit; FLJ45906; IDH3GL; NRP1; isocitrate dehydrogenase 3 (NAD+) gamma-like; nel-like 1; neural epidermal growth factor-like 1; NEL-like 1 (chicken)
 Product Gene Name   

NELL1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS900586 Testing Data    Testing Data PDF
 OMIM    602319
 3D Structure    ModBase 3D Structure for Q92832
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human NELL1. No significant cross-reactivity or interference between human NELL1 and analogues was observed.
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 Samples    Serum, plasma, cell culture supernates, tissue homogenates.
 Assay Type    Sandwich
 Detection Range    31.25 pg/ml -2000 pg/ml.
 Sensitivity    7.81 pg/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NELL1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NELL1 purchase    MBS900586 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the NEL-like 1 (chicken) (NELL1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25 pg/ml -2000 pg/ml in biological research samples containing NELL1, with an estimated sensitivity of 7.81 pg/ml. The ELISA analytical biochemical technique of the MBS900586 kit is based on NELL1 antibody-NELL1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NELL1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NELL1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for NELL1 elisa kit

   Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NELL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NELL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NELL1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NELL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of NELL1 elisa kit    NELL1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS900586. Click to request current manual
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NCBI/Uniprot data below describe general gene information for NELL1. It may not necessarily be applicable to this product.
 NCBI GI #    570359542
 NCBI GeneID    4745
 NCBI Accession #    NP_001275642.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001288713.1 [Other Products]
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 UniProt Primary Accession #    Q92832 [Other Products]
 UniProt Secondary Accession #    Q4VB90; Q4VB91; Q6NSY8; Q9Y472; B2CKC1 [Other Products]
 UniProt Related Accession #    Q92832 [Other Products]
 Molecular Weight    89,635 Da
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 NCBI Official Full Name    protein kinase C-binding protein NELL1 isoform 3
 NCBI Official Synonym Full Names    NEL-like 1 (chicken)
 NCBI Official Symbol    NELL1 [Similar Products]
 NCBI Official Synonym Symbols   
NRP1; IDH3GL
[Similar Products]
 NCBI Protein Information    protein kinase C-binding protein NELL1; nel-related protein 1; neural epidermal growth factor-like 1
 UniProt Protein Name    Protein kinase C-binding protein NELL1
 UniProt Synonym Protein Names   
NEL-like protein 1; Nel-related protein 1
 Protein Family    Protein kinase C-binding protein
 UniProt Gene Name    NELL1 [Similar Products]
 UniProt Synonym Gene Names    NRP1 [Similar Products]
 UniProt Entry Name    NELL1_HUMAN
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 NCBI Summary for NELL1    This gene encodes a cytoplasmic protein that contains epidermal growth factor (EGF)-like repeats. The encoded heterotrimeric protein may be involved in cell growth regulation and differentiation. A similar protein in rodents is involved in craniosynostosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]
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 UniProt Comments for NELL1    NELL1: Plays a role in the control of cell growth and differentiation. Promotes osteoblast cell differentiation and terminal mineralization. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: perinuclear region of cytoplasm; cytoplasm; extracellular region; nuclear envelope

Molecular Function: protein binding; calcium ion binding

Biological Process: positive regulation of osteoblast differentiation; nervous system development; regulation of gene expression; negative regulation of osteoblast proliferation; cell differentiation; positive regulation of bone mineralization
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 Research Articles on NELL1    1. NELL1 is able to promote the osteogenic differentiation of periodontal ligament stem cells, which may be related to the downregulation of Msx2 expression.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with NELL1 elisa kitDiseases associated with NELL1 elisa kit
 Reference Product  PubMed Publications
 RUNX2 elisa kit  >14 publications with NELL1 and RUNX2
 BMP7 elisa kit  >3 publications with NELL1 and BMP7
 SP7 elisa kit  >2 publications with NELL1 and SP7
 Disease Name  Pubmed Publications
 Congenital Abnormalities Antibodies  >10 publications with NELL1 and Congenital Abnormalities
 Craniosynostoses Antibodies  >7 publications with NELL1 and Craniosynostoses
 Intestinal Neoplasms Antibodies  >2 publications with NELL1 and Intestinal Neoplasms
 Necrosis Antibodies  >2 publications with NELL1 and Necrosis
 Disease Models, Animal Antibodies  >2 publications with NELL1 and Disease Models, Animal
 Fibrosis Antibodies  >1 publications with NELL1 and Fibrosis
 Motor Skills Disorders Antibodies  >1 publications with NELL1 and Motor Skills Disorders
 Rhabdomyosarcoma Antibodies  >1 publications with NELL1 and Rhabdomyosarcoma
 Carcinoma, Hepatocellular Antibodies  >1 publications with NELL1 and Carcinoma, Hepatocellular
 Inflammation Antibodies  >1 publications with NELL1 and Inflammation
Organs/Tissues associated with NELL1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >7 publications with NELL1 and Brain
 Kidney Antibodies  >5 publications with NELL1 and Kidney
 Connective Tissue Antibodies  >4 publications with NELL1 and Connective Tissue
 Muscle Antibodies  >4 publications with NELL1 and Muscle
 Testis Antibodies  >2 publications with NELL1 and Testis
 Blood Antibodies  >1 publications with NELL1 and Blood
 Lung Antibodies  >1 publications with NELL1 and Lung
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