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NLRP3 elisa kit :: Human NLR family, pyrin domain containing 3 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS917009
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

NLR family, pyrin domain containing 3 (NLRP3), ELISA Kit

★Popular Item★
 Also Known As   

Human NACHT, LRR and PYD domains-containing protein 3, NLRP3/C1orf7/CIAS1/NALP3/PYPAF1 ELISA Kit

 Product Synonym Names    Human NACHT; LRR and PYD domains-containing protein 3 (NLRP3/C1orf7/CIAS1/NALP3/PYPAF1) ELISA kit; AGTAVPRL; AII; AII/AVP; AVP; C1orf7; CIAS1; CLR1.1; FCAS; FCU; FLJ95925; MWS; NALP3; PYPAF1; AII/AVP receptor-like; NACHT domain-; leucine-rich repeat-; and PYD-containing protein 3; NACHT; LRR and PY; NLR family; pyrin domain containing 3
 Product Gene Name   

NLRP3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS917009 Testing Data    Testing Data PDF
 OMIM    gene 607115
 3D Structure    ModBase 3D Structure for Q96P20
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human NLRP3. No significant cross-reactivity or interference between human NLRP3 and analogues was observed.
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 Samples    Serum, cell culture supernates, cerebrospinal fluid (CSF)
 Detection Range    0.156 ng/ml -10 ng/mL
 Sensitivity    <0.039 ng/mL
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NLRP3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NLRP3 purchase    MBS917009 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the NLR family, pyrin domain containing 3 (NLRP3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156 ng/ml -10 ng/mL in biological research samples containing NLRP3, with an estimated sensitivity of <0.039 ng/mL. The ELISA analytical biochemical technique of the MBS917009 kit is based on NLRP3 antibody-NLRP3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NLRP3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NLRP3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, cell culture supernates, cerebrospinal fluid (CSF). Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for NLRP3 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NLRP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NLRP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NLRP3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NLRP3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of NLRP3 elisa kit    NLRP3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS917009. Click to request current manual
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NCBI/Uniprot data below describe general gene information for NLRP3. It may not necessarily be applicable to this product.
 NCBI GI #    119395764
 NCBI GeneID    114548
 NCBI Accession #    NP_001073289.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001079821.2 [Other Products]
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 UniProt Primary Accession #    Q96P20 [Other Products]
 UniProt Secondary Accession #    O75434; Q17RS2; Q59H68; Q5JQS8; Q5JQS9; Q6TG35; Q8TCW0; B2RC97; B7ZKS9; B7ZKT2; B7ZKT3 [Other Products]
 UniProt Related Accession #    Q96P20 [Other Products]
 Molecular Weight    118,173 Da
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 NCBI Official Full Name    NACHT, LRR and PYD domains-containing protein 3 isoform a
 NCBI Official Synonym Full Names    NLR family, pyrin domain containing 3
 NCBI Official Symbol    NLRP3 [Similar Products]
 NCBI Official Synonym Symbols   
AII; AVP; FCU; MWS; FCAS; CIAS1; NALP3; C1orf7; CLR1.1; PYPAF1; AGTAVPRL
[Similar Products]
 NCBI Protein Information    NACHT, LRR and PYD domains-containing protein 3; cryopyrin; caterpiller protein 1.1; PYRIN-containing APAF1-like protein 1; NACHT, LRR and PYD containing protein 3; cold autoinflammatory syndrome 1 protein; NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3
 UniProt Protein Name    NACHT, LRR and PYD domains-containing protein 3
 UniProt Synonym Protein Names   
Angiotensin/vasopressin receptor AII/AVP-like; Caterpiller protein 1.1; CLR1.1; Cold autoinflammatory syndrome 1 protein; Cryopyrin; PYRIN-containing APAF1-like protein 1
 Protein Family    NACHT, LRR and PYD domains-containing protein
 UniProt Gene Name    NLRP3 [Similar Products]
 UniProt Synonym Gene Names    C1orf7; CIAS1; NALP3; PYPAF1; CLR1.1 [Similar Products]
 UniProt Entry Name    NALP3_HUMAN
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 NCBI Summary for NLRP3    This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID). Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid. [provided by RefSeq, Oct 2008]
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 UniProt Comments for NLRP3    NLRP3: May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1); also known as familial cold urticaria. FCAS are rare autosomal dominant systemic inflammatory diseases characterized by episodes of rash, arthralgia, fever and conjunctivitis after generalized exposure to cold. Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS); also known as urticaria-deafness-amyloidosis syndrome. MWS is a hereditary periodic fever syndrome characterized by fever, chronic recurrent urticaria, arthralgias, progressive sensorineural deafness, and reactive renal amyloidosis. The disease may be severe if generalized amyloidosis occurs. Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA); also known as neonatal onset multisystem inflammatory disease (NOMID). CINCA is a rare congenital inflammatory disorder characterized by a triad of neonatal onset of cutaneous symptoms, chronic meningitis and joint manifestations with recurrent fever and inflammation. Belongs to the NLRP family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 1q44

Cellular Component: cytoplasm; cytosol

Molecular Function: peptidoglycan binding; protein binding; ATP binding

Biological Process: caspase activation; apoptosis; negative regulation of acute inflammatory response; positive regulation of caspase activity; interleukin-1 beta production; defense response; positive regulation of interleukin-1 beta secretion; negative regulation of NF-kappaB import into nucleus; signal transduction; protein oligomerization; activation of NF-kappaB transcription factor; negative regulation of interleukin-1 beta secretion; inhibition of NF-kappaB transcription factor; negative regulation of inflammatory response; interleukin-18 production; innate immune response; interleukin-1 secretion; inflammatory response; detection of biotic stimulus; defense response to virus

Disease: Cinca Syndrome; Familial Cold Autoinflammatory Syndrome 1; Muckle-wells Syndrome
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 Research Articles on NLRP3    1. NLRP3 inflammasome-initiated inflammation by controlling ROS production in astrocytes.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with NLRP3 elisa kitPathways associated with NLRP3 elisa kit
 Reference Product  PubMed Publications
 PYCARD elisa kit  >79 publications with NLRP3 and PYCARD
 NLRC4 elisa kit  >60 publications with NLRP3 and NLRC4
 CARD8 elisa kit  >15 publications with NLRP3 and CARD8
 Products by Pathway  Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Inflammasomes Pathway antibodies  Inflammasomes Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 NOD-like Receptor Signaling Pathway antibodies  NOD-like Receptor Signaling Pathway Diagram
 NOD-like Receptor Signaling Pathway antibodies  NOD-like Receptor Signaling Pathway Diagram
 Nucleotide-binding Domain, Leucine Rich Repeat Containing Receptor (NLR) Signaling Pathways antibodies  Nucleotide-binding Domain, Leucine Rich Repeat Containing Receptor (NLR) Signaling Pathways Diagram
 Pertussis Pathway antibodies  Pertussis Pathway Diagram
 Pertussis Pathway antibodies  Pertussis Pathway Diagram
Diseases associated with NLRP3 elisa kitOrgans/Tissues associated with NLRP3 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >389 publications with NLRP3 and Inflammation
 MUCKLE-WELLS SYNDROME Antibodies  >121 publications with NLRP3 and MUCKLE-WELLS SYNDROME
 Necrosis Antibodies  >80 publications with NLRP3 and Necrosis
 Cryopyrin-Associated Periodic Syndromes Antibodies  >72 publications with NLRP3 and Cryopyrin-Associated Periodic Syndromes
 FAMILIAL COLD AUTOINFLAMMATORY SYNDROME 1 Antibodies  >71 publications with NLRP3 and FAMILIAL COLD AUTOINFLAMMATORY SYNDROME 1
 Death Antibodies  >70 publications with NLRP3 and Death
 Disease Models, Animal Antibodies  >38 publications with NLRP3 and Disease Models, Animal
 Lung Diseases Antibodies  >33 publications with NLRP3 and Lung Diseases
 Gastrointestinal Diseases Antibodies  >32 publications with NLRP3 and Gastrointestinal Diseases
 Pain Antibodies  >27 publications with NLRP3 and Pain
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >182 publications with NLRP3 and Blood
 Lung Antibodies  >42 publications with NLRP3 and Lung
 Bone Marrow Antibodies  >35 publications with NLRP3 and Bone Marrow
 Brain Antibodies  >11 publications with NLRP3 and Brain
 Intestine Antibodies  >10 publications with NLRP3 and Intestine
 Connective Tissue Antibodies  >5 publications with NLRP3 and Connective Tissue
 Pancreas Antibodies  >4 publications with NLRP3 and Pancreas
 Thymus Antibodies  >1 publications with NLRP3 and Thymus
 Prostate Antibodies  >1 publications with NLRP3 and Prostate
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