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NOV elisa kit :: Human NOV/CCN3 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS177346
 Typical Testing Data/Standard Curve
Unit / Price
96-Strip-Wells  /  $425 +1 FREE 8GB USB
 
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 Product Name   

NOV/CCN3, ELISA Kit

★Popular Item★
 Also Known As   

Human NOV/CCN3 PicoKine ELISA Kit

 Product Synonym Names    Protein NOV homolog; nephroblastoma overexpressed; CCN family member 3; CCN3; IBP-9; IGF-binding protein 9; IGFBP-9; IGFBP9; Insulin-like growth factor-binding protein 9; Nephroblastoma overexpressed; Nephroblastoma overexpressed gene protein homolog; Nephroblastoma-overexpressed gene protein homolog; NOV; NOV_HUMAN; NovH; Protein NOV homolog
 Product Gene Name   

NOV elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    164958
 3D Structure    ModBase 3D Structure for P48745
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 Species Reactivity    Human
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 Specificity    Natural and recombinant Human NOV/CCN3
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 Samples    Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
 Assay Type    Quantitative Sandwich
 Detection Range    31.2pg/ml-2000pg/ml
 Sensitivity    <2pg/ml
 Intra-assay Precision    Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
 Inter-assay Precision    Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
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 Preparation and Storage    Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NOV elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NOV purchase    MBS177346 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the NOV/CCN3, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.2pg/ml-2000pg/ml in biological research samples containing NOV, with an estimated sensitivity of <2pg/ml. The ELISA analytical biochemical technique of the MBS177346 kit is based on NOV antibody-NOV antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NOV antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NOV. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for NOV elisa kit

   Principle of the Assay: Human NOV/CCN3 ELISA Kit was based on standard sandwich enzyme-linked immunesorbent assay technology. A monoclonal antibody from mouse specific for NOV/CCN3 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: T32-M357) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for NOV/CCN3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human NOV/CCN3 amount of sample captured in plate.

Background/Introduction: NOV(nephroblastoma overexpressed), also known as CCN3, is a matricellular protein that in humans is encoded by the NOV gene. The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. This gene is mapped to 8q24.12. NOV is a potentially useful marker for the diagnosis of adrenal gland diseases, malignant adrenocortical tumors, multiple sclerosis and so on. Moreover, reduced expression of NOV in ACTs may play an important role in the process of childhood ACT tumorigenesis. Though studying, it identified Nov as a regulator of human hematopoietic stem or progenitor cells.
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  Typical Testing Data/Standard Curve of NOV elisa kit    Human NOV/CCN3 PicoKine ELISA Kit standard curve
NOV elisa kit  Typical Testing Data/Standard Curve image
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Sample Manual Insert of MBS177346. Click to request current manual
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NCBI/Uniprot data below describe general gene information for NOV. It may not necessarily be applicable to this product.
 NCBI GI #    1352515
 NCBI GeneID    4856
 NCBI Accession #    P48745.1 [Other Products]
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 UniProt Primary Accession #    P48745 [Other Products]
 UniProt Secondary Accession #    Q6I9S3; Q96BY5; Q9UDE4; B2R5X7 [Other Products]
 UniProt Related Accession #    P48745 [Other Products]
 Molecular Weight    39,162 Da
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 NCBI Official Full Name    Protein NOV homolog
 NCBI Official Synonym Full Names    nephroblastoma overexpressed
 NCBI Official Symbol    NOV [Similar Products]
 NCBI Official Synonym Symbols   
CCN3; NOVh; IBP-9; IGFBP9; IGFBP-9
[Similar Products]
 NCBI Protein Information    protein NOV homolog; CCN family member 3; IGF-binding protein 9; insulin-like growth factor-binding protein 9; nephroblastoma-overexpressed gene protein homolog
 UniProt Protein Name    Protein NOV homolog
 UniProt Synonym Protein Names   
CCN family member 3; Insulin-like growth factor-binding protein 9; IBP-9; IGF-binding protein 9; IGFBP-9; Nephroblastoma-overexpressed gene protein homolog
 Protein Family    Protein
 UniProt Gene Name    NOV [Similar Products]
 UniProt Synonym Gene Names    CCN3; IGFBP9; NOVH; NovH; IBP-9; IGF-binding protein 9; IGFBP-9 [Similar Products]
 UniProt Entry Name    NOV_HUMAN
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 NCBI Summary for NOV    The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. CNN family proteins associate with the extracellular matrix and play an important role in cardiovascular and skeletal development, fibrosis and cancer development. [provided by RefSeq, Feb 2009]
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 UniProt Comments for NOV    NOV: Immediate-early protein likely to play a role in cell growth regulation. Belongs to the CCN family. Expression is down-regulated by WT1. Interacts with FBLN1.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8q24.1

Cellular Component: proteinaceous extracellular matrix; cell soma; intracellular membrane-bound organelle; axon; dendrite

Molecular Function: heparin binding; integrin binding; insulin-like growth factor binding; growth factor activity

Biological Process: cell-cell signaling; regulation of gene expression; regulation of cell growth; cell adhesion; signal transduction
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 Research Articles on NOV    1. NOV may promote carcinogenesis via promotion of EMT and association with increased mTOR activity
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with NOV elisa kitPathways associated with NOV elisa kit
 Reference Product  PubMed Publications
 BMP2 elisa kit  >310 publications with NOV and BMP2
 TEC elisa kit  >211 publications with NOV and TEC
 MUSK elisa kit  >172 publications with NOV and MUSK
 GJA1 elisa kit  >48 publications with NOV and GJA1
 DDR1 elisa kit  >16 publications with NOV and DDR1
 PLXNA1 elisa kit  >4 publications with NOV and PLXNA1
 Products by Pathway  Pathway Diagram
 Delta-Notch Signaling Pathway antibodies  Delta-Notch Signaling Pathway Diagram
Diseases associated with NOV elisa kitOrgans/Tissues associated with NOV elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >152866 publications with NOV and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >147436 publications with NOV and Cardiovascular Diseases
 Vascular Diseases Antibodies  >105927 publications with NOV and Vascular Diseases
 Heart Diseases Antibodies  >75037 publications with NOV and Heart Diseases
 Brain Diseases Antibodies  >73666 publications with NOV and Brain Diseases
 Carcinoma Antibodies  >53970 publications with NOV and Carcinoma
 Kidney Diseases Antibodies  >33188 publications with NOV and Kidney Diseases
 Inflammation Antibodies  >33166 publications with NOV and Inflammation
 Hypertension Antibodies  >28399 publications with NOV and Hypertension
 Adenocarcinoma Antibodies  >24055 publications with NOV and Adenocarcinoma
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >262213 publications with NOV and Blood
 Brain Antibodies  >107412 publications with NOV and Brain
 Heart Antibodies  >88287 publications with NOV and Heart
 Vascular Antibodies  >78238 publications with NOV and Vascular
 Bone Antibodies  >72826 publications with NOV and Bone
 Liver Antibodies  >67084 publications with NOV and Liver
 Muscle Antibodies  >65477 publications with NOV and Muscle
 Kidney Antibodies  >50806 publications with NOV and Kidney
 Lung Antibodies  >49418 publications with NOV and Lung
 Skin Antibodies  >45964 publications with NOV and Skin
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