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NKCC2 elisa kit :: Human Na-K-Cl Cotransporter 2 (NKCC2) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS453691
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $400 +1 FREE 8GB USB
96-Strip-Wells  /  $535 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,425 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,710 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Na-K-Cl Cotransporter 2 (NKCC2), ELISA Kit

★Popular Item★
 Also Known As   

Human Na-K-Cl Cotransporter 2 (NKCC2) ELISA Kit

 Product Synonym Names    SLC12A1; NKCC2; Solute Carrier Family 12 Member 1; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; Kidney-specific Na-K-Cl symporter
 Product Gene Name   

NKCC2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    U58130 mRNA
 3D Structure    ModBase 3D Structure for Q13621
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of NKCC2. No significant cross-reactivity or interference between NKCC2 and analogues was observed.
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 Samples    Tissue homogenates, cell lysates and other biological fluids.
 Assay Type    Sandwich
 Detection Range    31.25-2000pg/mL
 Sensitivity    13.8pg/mL
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NKCC2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NKCC2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
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 Preparation and Storage    For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NKCC2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NKCC2 purchase    MBS453691 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Na-K-Cl Cotransporter 2 (NKCC2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25-2000pg/mL in biological research samples containing NKCC2, with an estimated sensitivity of 13.8pg/mL. The ELISA analytical biochemical technique of the MBS453691 kit is based on NKCC2 antibody-NKCC2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NKCC2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NKCC2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates, cell lysates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for NKCC2 elisa kit

   Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NKCC2 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NKCC2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NKCC2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NKCC2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NKCC2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of NKCC2 elisa kit    NKCC2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS453691. Click to request current manual
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NCBI/Uniprot data below describe general gene information for NKCC2. It may not necessarily be applicable to this product.
 NCBI GI #    134254459
 NCBI GeneID    6557
 NCBI Accession #    NP_000329.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000338.2 [Other Products]
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 UniProt Primary Accession #    Q13621 [Other Products]
 UniProt Secondary Accession #    A8JYA2; E9PDW4 [Other Products]
 UniProt Related Accession #    Q13621 [Other Products]
 Molecular Weight    – Da
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 NCBI Official Full Name    solute carrier family 12 member 1 isoform A
 NCBI Official Synonym Full Names    solute carrier family 12 member 1
 NCBI Official Symbol    SLC12A1 [Similar Products]
 NCBI Official Synonym Symbols   
BSC1; NKCC2
[Similar Products]
 NCBI Protein Information    solute carrier family 12 member 1
 UniProt Protein Name    Solute carrier family 12 member 1
 UniProt Synonym Protein Names   
Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; Kidney-specific Na-K-Cl symporter
 UniProt Gene Name    SLC12A1 [Similar Products]
 UniProt Synonym Gene Names    NKCC2 [Similar Products]
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 NCBI Summary for NKCC2    This gene encodes a kidney-specific sodium-potassium-chloride cotransporter that is expressed on the luminal membrane of renal epithelial cells of the thick ascending limb of Henle's loop and the macula densa. It plays a key role in concentrating urine and accounts for most of the NaCl resorption. It is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their biological validity in humans has not been experimentally proven.[provided by RefSeq, May 2010]
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 UniProt Comments for NKCC2    Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume.
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 Research Articles on NKCC2    1. Low SLC12A1 urine levels were associated with Bartter syndrome.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with NKCC2 elisa kitPathways associated with NKCC2 elisa kit
 Reference Product  PubMed Publications
 AQP2 elisa kit  >52 publications with NKCC2 and AQP2
 KCNJ1 elisa kit  >41 publications with NKCC2 and KCNJ1
 CLCNKB elisa kit  >17 publications with NKCC2 and CLCNKB
 STK39 elisa kit  >17 publications with NKCC2 and STK39
 AVP elisa kit  >16 publications with NKCC2 and AVP
 WNK1 elisa kit  >14 publications with NKCC2 and WNK1
 WNK4 elisa kit  >10 publications with NKCC2 and WNK4
 WNK3 elisa kit  >6 publications with NKCC2 and WNK3
 SCAMP2 elisa kit  >2 publications with NKCC2 and SCAMP2
 Products by Pathway  Pathway Diagram
 Cation-coupled Chloride Cotransporters Pathway antibodies  Cation-coupled Chloride Cotransporters Pathway Diagram
 SLC-mediated Transmembrane Transport Pathway antibodies  SLC-mediated Transmembrane Transport Pathway Diagram
 Transmembrane Transport Of Small Molecules Pathway antibodies  Transmembrane Transport Of Small Molecules Pathway Diagram
 Transport Of Inorganic Cations/anions And Amino Acids/oligopeptides Pathway antibodies  Transport Of Inorganic Cations/anions And Amino Acids/oligopeptides Pathway Diagram
Diseases associated with NKCC2 elisa kitOrgans/Tissues associated with NKCC2 elisa kit
 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >92 publications with NKCC2 and Kidney Diseases
 Hypertension Antibodies  >84 publications with NKCC2 and Hypertension
 Bartter Syndrome Antibodies  >50 publications with NKCC2 and Bartter Syndrome
 Disease Models, Animal Antibodies  >29 publications with NKCC2 and Disease Models, Animal
 Hypokalemia Antibodies  >27 publications with NKCC2 and Hypokalemia
 Polyuria Antibodies  >27 publications with NKCC2 and Polyuria
 Renal Insufficiency Antibodies  >14 publications with NKCC2 and Renal Insufficiency
 Brain Diseases Antibodies  >10 publications with NKCC2 and Brain Diseases
 Hypotension Antibodies  >8 publications with NKCC2 and Hypotension
 Proteinuria Antibodies  >7 publications with NKCC2 and Proteinuria
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >269 publications with NKCC2 and Kidney
 Vascular Antibodies  >29 publications with NKCC2 and Vascular
 Brain Antibodies  >18 publications with NKCC2 and Brain
 Parathyroid Antibodies  >12 publications with NKCC2 and Parathyroid
 Intestine Antibodies  >10 publications with NKCC2 and Intestine
 Muscle Antibodies  >10 publications with NKCC2 and Muscle
 Liver Antibodies  >9 publications with NKCC2 and Liver
 Lung Antibodies  >8 publications with NKCC2 and Lung
 Bone Antibodies  >8 publications with NKCC2 and Bone
 Eye Antibodies  >4 publications with NKCC2 and Eye
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