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NPR1 elisa kit :: Human Natriuretic Peptide Receptor 1 (NPR1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2019629 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $380 +1 FREE 8GB USB
48-Strip-Wells  /  $565 +1 FREE 8GB USB
96-Strip-Wells  /  $765 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,130 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,670 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Natriuretic Peptide Receptor 1 (NPR1), ELISA Kit

 Also Known As   

Natriuretic Peptide Receptor 1 (NPR1) ELISA Kit

 Product Synonym Names    ANPRA; ANPa; GUC2A; GUCY2A; NPR-A; Guanylate Cyclase A; Atrionatriuretic Peptide Receptor A; Atrial natriuretic peptide receptor type A
 Product Gene Name   

NPR1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for P18293
 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Natriuretic Peptide Receptor 1 (NPR1).
No significant cross-reactivity or interference between Natriuretic Peptide Receptor 1 (NPR1) and analogues was observed.
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 Samples    Tissue homogenates, Cell lysates and other biological fluids
 Assay Type    Sandwich
 Detection Range    0.313-20ng/mL
 Sensitivity    Typically less than 0.115ng/mL.
 Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Natriuretic Peptide Receptor 1 (NPR1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Natriuretic Peptide Receptor 1 (NPR1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
 Assay Procedure Summary    1. Prepare all reagents, samples and standards;
2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;
3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;
4. Aspirate and wash 3 times;
5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;
6. Aspirate and wash 5 times;
7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;
8. Add 50uL Stop Solution. Read at 450nm immediately.
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 Preparation and Storage    For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C.

For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NPR1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NPR1 purchase    MBS2019629 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Natriuretic Peptide Receptor 1 (NPR1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.313-20ng/mL in biological research samples containing NPR1, with an estimated sensitivity of Typically less than 0.115ng/mL. The ELISA analytical biochemical technique of the MBS2019629 kit is based on NPR1 antibody-NPR1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NPR1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NPR1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates, Cell lysates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for NPR1 elisa kit

   Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Natriuretic Peptide Receptor 1 (NPR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Natriuretic Peptide Receptor 1 (NPR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Natriuretic Peptide Receptor 1 (NPR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Natriuretic Peptide Receptor 1 (NPR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of NPR1 elisa kit    NPR1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2019629. Click to request current manual
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NCBI/Uniprot data below describe general gene information for NPR1. It may not necessarily be applicable to this product.
 NCBI GI #    148683193
 NCBI GeneID    18160
 NCBI Accession #    EDL15140.1 [Other Products]
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 UniProt Primary Accession #    P18293 [Other Products]
 UniProt Related Accession #    P18293 [Other Products]
 Molecular Weight    119,109 Da
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 NCBI Official Full Name    natriuretic peptide receptor 1
 NCBI Official Synonym Full Names    natriuretic peptide receptor 1
 NCBI Official Symbol    Npr1 [Similar Products]
 NCBI Official Synonym Symbols   
GC-A; NPRA; Pndr; NPR-A; AI893888
[Similar Products]
 NCBI Protein Information    atrial natriuretic peptide receptor 1
 UniProt Protein Name    Atrial natriuretic peptide receptor 1
 UniProt Synonym Protein Names   
Atrial natriuretic peptide receptor type A; ANP-A; ANPR-A; NPR-A; Guanylate cyclase A; GC-A
 Protein Family    Nitrogen permease reactivator protein
 UniProt Gene Name    Npr1 [Similar Products]
 UniProt Synonym Gene Names    Npra; ANP-A; ANPR-A; NPR-A; GC-A [Similar Products]
 UniProt Entry Name    ANPRA_MOUSE
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 UniProt Comments for NPR1    ANPA: natriuretic peptide receptor A, which mediates natriuretic, diuretic, and vasorelaxing actions of the natriuretic peptides. Contains five functional domains: an extracellular ligand-binding domain, a single membrane-spanning region, and intracellularly a protein kinase homology domain, a helical hinge region involved in oligomerization, and a carboxyl-terminal guanylyl cyclase catalytic domain. Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Has guanylate cyclase activity on binding of ANF. Hypertensive SHR Rat has polymorphism at the locus. Human non-coding polymorphisms can alter expression up to two-fold, and have been associated with hypertension. A coding SNP is associated with hypertension and myocardial infarction.

Protein type: Protein kinase, dual-specificity (receptor); Guanylyl cyclase; Lyase; Membrane protein, integral; Receptor, misc.; Kinase, protein; Protein kinase, RGC; Nucleotide Metabolism - purine; EC 4.6.1.2; RGC group; RGC family

Cellular Component: membrane; integral to membrane; plasma membrane; intracellular; receptor complex

Molecular Function: guanylate cyclase activity; hormone binding; GTP binding; phosphorus-oxygen lyase activity; natriuretic peptide receptor activity; peptide hormone binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; lyase activity; protein kinase binding; ATP binding; protein kinase activity

Biological Process: cGMP biosynthetic process; cyclic nucleotide biosynthetic process; cell surface receptor linked signal transduction; positive regulation of cGMP biosynthetic process; negative regulation of smooth muscle cell proliferation; receptor guanylyl cyclase signaling pathway; regulation of blood pressure; regulation of blood vessel size; protein amino acid phosphorylation; dopamine metabolic process
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 Research Articles on NPR1    1. these findings define a novel epigenetic regulatory mechanism that governs Npr1 gene transcription.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with NPR1 elisa kitDiseases associated with NPR1 elisa kit
 Products by Pathway  Pathway Diagram
 Oxytocin Signaling Pathway antibodies  Oxytocin Signaling Pathway Diagram
 Oxytocin Signaling Pathway antibodies  Oxytocin Signaling Pathway Diagram
 PluriNetWork Pathway antibodies  PluriNetWork Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Vascular Smooth Muscle Contraction Pathway antibodies  Vascular Smooth Muscle Contraction Pathway Diagram
 Vascular Smooth Muscle Contraction Pathway antibodies  Vascular Smooth Muscle Contraction Pathway Diagram
 XPodNet - Protein-protein Interactions In The Podocyte Expanded By STRING Pathway antibodies  XPodNet - Protein-protein Interactions In The Podocyte Expanded By STRING Pathway Diagram
 CAMP Signaling Pathway antibodies  CAMP Signaling Pathway Diagram
 Disease Name  Pubmed Publications
 Hypertension Antibodies  >23 publications with NPR1 and Hypertension
 Hypertrophy Antibodies  >21 publications with NPR1 and Hypertrophy
 Heart Diseases Antibodies  >19 publications with NPR1 and Heart Diseases
 Necrosis Antibodies  >15 publications with NPR1 and Necrosis
 Fibrosis Antibodies  >14 publications with NPR1 and Fibrosis
 Cardiomegaly Antibodies  >13 publications with NPR1 and Cardiomegaly
 Heart Failure Antibodies  >9 publications with NPR1 and Heart Failure
 Inflammation Antibodies  >7 publications with NPR1 and Inflammation
 Brain Diseases Antibodies  >3 publications with NPR1 and Brain Diseases
 Kidney Diseases Antibodies  >3 publications with NPR1 and Kidney Diseases
Organs/Tissues associated with NPR1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >20 publications with NPR1 and Brain
 Kidney Antibodies  >18 publications with NPR1 and Kidney
 Embryonic Tissue Antibodies  >4 publications with NPR1 and Embryonic Tissue
 Adrenal Gland Antibodies  >3 publications with NPR1 and Adrenal Gland
 Lung Antibodies  >3 publications with NPR1 and Lung
 Liver Antibodies  >2 publications with NPR1 and Liver
 Salivary Gland Antibodies  >1 publications with NPR1 and Salivary Gland
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