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NPHN elisa kit :: Human Nephrin (NPHN) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2022646 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $265 +1 FREE 8GB USB
48-Strip-Wells  /  $515 +1 FREE 8GB USB
96-Strip-Wells  /  $695 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,840 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,180 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Nephrin (NPHN), ELISA Kit

 Also Known As   

Nephrin (NPHN) ELISA Kit

 Product Synonym Names    NPHS1; CNF; Nephrosis 1; Congenital Finnish Type; Renal glomerulus-specific cell adhesion receptor
 Product Gene Name   

NPHN elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 OMIM    256300
 3D Structure    ModBase 3D Structure for O60500
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Nephrin (NPHN).
No significant cross-reactivity or interference between Nephrin (NPHN) and analogues was observed.
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 Samples    Tissue homogenates, urine, Cell lysates, cell culture supernates and other biological fluids
 Assay Type    Sandwich
 Detection Range    0.156-10ng/mL
 Sensitivity    Typically less than 0.057ng/mL.
 Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nephrin (NPHN) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nephrin (NPHN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
 Assay Procedure Summary    1. Prepare all reagents, samples and standards;
2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;
3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;
4. Aspirate and wash 3 times;
5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;
6. Aspirate and wash 5 times;
7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;
8. Add 50uL Stop Solution. Read at 450nm immediately.
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 Preparation and Storage    For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C.

For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Other Notes    Small volumes of NPHN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NPHN purchase    MBS2022646 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Nephrin (NPHN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing NPHN, with an estimated sensitivity of Typically less than 0.057ng/mL. The ELISA analytical biochemical technique of the MBS2022646 kit is based on NPHN antibody-NPHN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NPHN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NPHN. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions such as Tissue homogenates, urine, Cell lysates, cell culture supernates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for NPHN elisa kit

   Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nephrin (NPHN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nephrin (NPHN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nephrin (NPHN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nephrin (NPHN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of NPHN elisa kit    NPHN elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2022646
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NCBI/Uniprot data below describe general gene information for NPHN. It may not necessarily be applicable to this product.
 NCBI GI #    10441644
 NCBI GeneID    4868
 NCBI Accession #    AAG17141.1 [Other Products]
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 UniProt Primary Accession #    O60500 [Other Products]
 UniProt Secondary Accession #    A6NDH2; C3RX61 [Other Products]
 UniProt Related Accession #    O60500 [Other Products]
 Molecular Weight    130,546 Da
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 NCBI Official Full Name    nephrin
 NCBI Official Synonym Full Names    nephrosis 1, congenital, Finnish type (nephrin)
 NCBI Official Symbol    NPHS1 [Similar Products]
 NCBI Official Synonym Symbols   
CNF; NPHN; nephrin
[Similar Products]
 NCBI Protein Information    nephrin
 UniProt Protein Name    Nephrin
 UniProt Synonym Protein Names   
Renal glomerulus-specific cell adhesion receptor
 UniProt Gene Name    NPHS1 [Similar Products]
 UniProt Synonym Gene Names    NPHN [Similar Products]
 UniProt Entry Name    NPHN_HUMAN
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 NCBI Summary for NPHN    This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.[provided by RefSeq, Oct 2009]
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 UniProt Comments for NPHN    NPHS1: Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion. Interacts with CD2AP (via C-terminal domain). Interacts with MAGI1 (via PDZ 2 and 3 domains) forming a tripartite complex with IGSF5/JAM4. Interacts with DDN; the interaction is direct. Self-associates (via the Ig-like domains). Also interacts (via the Ig-like domains) with KIRREL/NEPH1 and KIRREL2; the interaction with KIRREL is dependent on KIRREL glycosylation. Forms a complex with ACTN4, CASK, IQGAP1, MAGI2, SPTAN1 and SPTBN1. Interacts with NPHS2. Specifically expressed in podocytes of kidney glomeruli. Belongs to the immunoglobulin superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion; Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: protein complex; cell projection; integral to plasma membrane; plasma membrane; lipid raft

Molecular Function: protein domain specific binding; protein binding; myosin binding; spectrin binding; alpha-actinin binding

Biological Process: skeletal muscle development; positive regulation of actin filament polymerization; glomerular basement membrane development; JNK cascade; cell adhesion; excretion; myoblast fusion

Disease: Nephrotic Syndrome, Type 1
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 Research Articles on NPHN    1. biochemical reconstitution on supported lipid bilayers of protein clusters containing the adhesion receptor Nephrin and its cytoplasmic partners, Nck and N-WASP, is reported.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with NPHN elisa kitPathways associated with NPHN elisa kit
 Reference Product  PubMed Publications
 NPHS2 elisa kit  >78 publications with NPHN and NPHS2
 CD2AP elisa kit  >24 publications with NPHN and CD2AP
 TRPC6 elisa kit  >8 publications with NPHN and TRPC6
 TJP1 elisa kit  >6 publications with NPHN and TJP1
 SRC elisa kit  >4 publications with NPHN and SRC
 KIRREL elisa kit  >4 publications with NPHN and KIRREL
 FYN elisa kit  >1 publications with NPHN and FYN
 Products by Pathway  Pathway Diagram
 Cell-Cell Communication Pathway antibodies  Cell-Cell Communication Pathway Diagram
 Nephrin Interactions Pathway antibodies  Nephrin Interactions Pathway Diagram
 Nephrin/Neph1 Signaling In The Kidney Podocyte Pathway antibodies  Nephrin/Neph1 Signaling In The Kidney Podocyte Pathway Diagram
Diseases associated with NPHN elisa kitOrgans/Tissues associated with NPHN elisa kit
 Disease Name  Pubmed Publications
 Nephrotic Syndrome Antibodies  >142 publications with NPHN and Nephrotic Syndrome
 Kidney Diseases Antibodies  >142 publications with NPHN and Kidney Diseases
 Nephrosis Antibodies  >112 publications with NPHN and Nephrosis
 Proteinuria Antibodies  >76 publications with NPHN and Proteinuria
 Glomerulonephritis Antibodies  >37 publications with NPHN and Glomerulonephritis
 Glomerulosclerosis, Focal Segmental Antibodies  >37 publications with NPHN and Glomerulosclerosis, Focal Segmental
 Renal Insufficiency Antibodies  >16 publications with NPHN and Renal Insufficiency
 Kidney Failure, Chronic Antibodies  >15 publications with NPHN and Kidney Failure, Chronic
 Disease Models, Animal Antibodies  >12 publications with NPHN and Disease Models, Animal
 Hypertension Antibodies  >9 publications with NPHN and Hypertension
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >131 publications with NPHN and Kidney
 Pancreas Antibodies  >8 publications with NPHN and Pancreas
 Brain Antibodies  >6 publications with NPHN and Brain
 Thymus Antibodies  >3 publications with NPHN and Thymus
 Testis Antibodies  >2 publications with NPHN and Testis
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