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NPHN elisa kit :: Human Nephrin (NPHN) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS265927
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $260 +1 FREE 8GB USB
96-Strip-Wells  /  $405 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $2,880 +3 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Nephrin (NPHN), ELISA Kit

★Popular Item★
 Also Known As   

Human Nephrin (NPHN) ELISA Kit

 Product Gene Name   

NPHN elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    602716
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 Species Reactivity    Human
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 Specificity    No cross-reaction with other factors.
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 Samples    Serum, plasma or cell culture supernatant
 Assay Type    Quantitative Sandwich
 Detection Range    20 ng/ml-0.312 ng/ml
 Sensitivity    Up to 0.06 ng/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NPHN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NPHN purchase    MBS265927 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Nephrin (NPHN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 20 ng/ml-0.312 ng/ml in biological research samples containing NPHN, with an estimated sensitivity of Up to 0.06 ng/ml. The ELISA analytical biochemical technique of the MBS265927 kit is based on NPHN antibody-NPHN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NPHN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NPHN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma or cell culture supernatant. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for NPHN elisa kit

   Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human NPHN monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Standard Curve/Testing Data of NPHN elisa kit    NPHN elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS265927. Click to request current manual
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NCBI/Uniprot data below describe general gene information for NPHN. It may not necessarily be applicable to this product.
 NCBI GI #    10441644
 NCBI GeneID    4868
 NCBI Accession #    AAG17141.1 [Other Products]
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 UniProt Secondary Accession #    A6NDH2; C3RX61 [Other Products]
 UniProt Related Accession #    O60500 [Other Products]
 Molecular Weight    130,546 Da
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 NCBI Official Full Name    nephrin
 NCBI Official Synonym Full Names    nephrosis 1, congenital, Finnish type (nephrin)
 NCBI Official Symbol    NPHS1 [Similar Products]
 NCBI Official Synonym Symbols   
CNF; NPHN; nephrin
[Similar Products]
 NCBI Protein Information    nephrin; renal glomerulus-specific cell adhesion receptor
 UniProt Protein Name    Nephrin
 UniProt Synonym Protein Names   
Renal glomerulus-specific cell adhesion receptor
 UniProt Gene Name    NPHS1 [Similar Products]
 UniProt Synonym Gene Names    NPHN [Similar Products]
 UniProt Entry Name    NPHN_HUMAN
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 NCBI Summary for NPHN    This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.[provided by RefSeq, Oct 2009]
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 UniProt Comments for NPHN    NPHS1: Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion. Interacts with CD2AP (via C-terminal domain). Interacts with MAGI1 (via PDZ 2 and 3 domains) forming a tripartite complex with IGSF5/JAM4. Interacts with DDN; the interaction is direct. Self-associates (via the Ig-like domains). Also interacts (via the Ig-like domains) with KIRREL/NEPH1 and KIRREL2; the interaction with KIRREL is dependent on KIRREL glycosylation. Forms a complex with ACTN4, CASK, IQGAP1, MAGI2, SPTAN1 and SPTBN1. Interacts with NPHS2. Specifically expressed in podocytes of kidney glomeruli. Belongs to the immunoglobulin superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Immunoglobulin superfamily; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: protein complex; cell projection; integral to plasma membrane; plasma membrane; lipid raft

Molecular Function: protein domain specific binding; protein binding; myosin binding; spectrin binding; alpha-actinin binding

Biological Process: skeletal muscle development; positive regulation of actin filament polymerization; glomerular basement membrane development; JNK cascade; excretion; cell adhesion; myoblast fusion

Disease: Nephrotic Syndrome, Type 1
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 Research Articles on NPHN    1. the frequency of identified disease causing mutations (NPHS1 and NPHS2) in children with steroid-resistant nephrotic syndome is 11.4%, and they show no response to treatment.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with NPHN elisa kitPathways associated with NPHN elisa kit
 Reference Product  PubMed Publications
 NPHS2 elisa kit  >66 publications with NPHN and NPHS2
 CD2AP elisa kit  >22 publications with NPHN and CD2AP
 TJP1 elisa kit  >6 publications with NPHN and TJP1
 TRPC6 elisa kit  >6 publications with NPHN and TRPC6
 SRC elisa kit  >4 publications with NPHN and SRC
 KIRREL elisa kit  >4 publications with NPHN and KIRREL
 FYN elisa kit  >1 publications with NPHN and FYN
 Products by Pathway  Pathway Diagram
 Cell-Cell Communication Pathway antibodies  Cell-Cell Communication Pathway Diagram
 Nephrin Interactions Pathway antibodies  Nephrin Interactions Pathway Diagram
 Nephrin/Neph1 Signaling In The Kidney Podocyte Pathway antibodies  Nephrin/Neph1 Signaling In The Kidney Podocyte Pathway Diagram
Diseases associated with NPHN elisa kitOrgans/Tissues associated with NPHN elisa kit
 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >124 publications with NPHN and Kidney Diseases
 Nephrosis Antibodies  >97 publications with NPHN and Nephrosis
 Proteinuria Antibodies  >71 publications with NPHN and Proteinuria
 Glomerulonephritis Antibodies  >33 publications with NPHN and Glomerulonephritis
 Glomerulosclerosis, Focal Segmental Antibodies  >30 publications with NPHN and Glomerulosclerosis, Focal Segmental
 Renal Insufficiency Antibodies  >14 publications with NPHN and Renal Insufficiency
 Kidney Failure, Chronic Antibodies  >13 publications with NPHN and Kidney Failure, Chronic
 Hypertension Antibodies  >8 publications with NPHN and Hypertension
 Albuminuria Antibodies  >6 publications with NPHN and Albuminuria
 Fibrosis Antibodies  >5 publications with NPHN and Fibrosis
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >118 publications with NPHN and Kidney
 Pancreas Antibodies  >8 publications with NPHN and Pancreas
 Brain Antibodies  >6 publications with NPHN and Brain
 Thymus Antibodies  >3 publications with NPHN and Thymus
 Testis Antibodies  >2 publications with NPHN and Testis
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