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NEFL elisa kit :: Human Neurofilament, Light Polypeptide (NEFL) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2020286 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $270 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $665 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,715 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,895 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Neurofilament, Light Polypeptide (NEFL), ELISA Kit

★Popular Item★
 Also Known As   

Neurofilament, Light Polypeptide (NEFL) ELISA Kit

 Product Synonym Names    CMT1F; CMT2E; NF-L; NF68; NFL; 68 kDa neurofilament protein; Neurofilament triplet L protein
 Product Gene Name   

NEFL elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    162280
 3D Structure    ModBase 3D Structure for P07196
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Neurofilament, Light Polypeptide (NEFL).
No significant cross-reactivity or interference between Neurofilament, Light Polypeptide (NEFL) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma, Cerebrospinal fluid and Other Biological Fluids
 Detection Range    15.6-1,000pg/mL
 Sensitivity    < 6.2pg/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Neurofilament, Light Polypeptide (NEFL) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Neurofilament, Light Polypeptide (NEFL) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NEFL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NEFL purchase    MBS2020286 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Neurofilament, Light Polypeptide (NEFL) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6-1,000pg/mL in biological research samples containing NEFL, with an estimated sensitivity of < 6.2pg/mL. The ELISA analytical biochemical technique of the MBS2020286 kit is based on NEFL antibody-NEFL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NEFL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NEFL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Cerebrospinal fluid and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for NEFL elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neurofilament, Light Polypeptide (NEFL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neurofilament, Light Polypeptide (NEFL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neurofilament, Light Polypeptide (NEFL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neurofilament, Light Polypeptide (NEFL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for NEFL elisa kit    Neuroscience
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 Typical Testing Data/Standard Curve (for reference only) of NEFL elisa kit    NEFL elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2020286. Click to request current manual
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NCBI/Uniprot data below describe general gene information for NEFL. It may not necessarily be applicable to this product.
 NCBI GI #    105990539
 NCBI GeneID    4747
 NCBI Accession #    NP_006149.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_006158.4 [Other Products]
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 UniProt Primary Accession #    P07196 [Other Products]
 UniProt Secondary Accession #    Q16154; Q8IU72; B9ZVN2 [Other Products]
 UniProt Related Accession #    P07196 [Other Products]
 Molecular Weight    61,517 Da
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 NCBI Official Full Name    neurofilament light polypeptide
 NCBI Official Synonym Full Names    neurofilament, light polypeptide
 NCBI Official Symbol    NEFL [Similar Products]
 NCBI Official Synonym Symbols   
NFL; NF-L; NF68; CMT1F; CMT2E; PPP1R110
[Similar Products]
 NCBI Protein Information    neurofilament light polypeptide
 UniProt Protein Name    Neurofilament light polypeptide
 UniProt Synonym Protein Names   
68 kDa neurofilament protein; Neurofilament triplet L protein
 Protein Family    Neurofilament light polypeptide
 UniProt Gene Name    NEFL [Similar Products]
 UniProt Synonym Gene Names    NF68; NFL; NF-L [Similar Products]
 UniProt Entry Name    NFL_HUMAN
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 NCBI Summary for NEFL    Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]
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 UniProt Comments for NEFL    NFL: one of the three (L, M, and H) intermediate filament proteins that form neurofilaments. Neurofilaments are involved in the maintenance of neuronal caliber. NF-L is the most abundant of the three neurofilament proteins. Defects cause Charcot-Marie-Tooth disease type 1F (CMT1F).

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 8p21

Cellular Component: TSC1-TSC2 complex; growth cone; axon; cytoplasm; neurofilament; cytosol

Molecular Function: protein C-terminus binding; protein binding, bridging; protein domain specific binding; identical protein binding; protein binding; phospholipase binding; structural constituent of cytoskeleton

Biological Process: protein polymerization; response to peptide hormone stimulus; neurofilament bundle assembly; response to toxin; intermediate filament organization; microtubule cytoskeleton organization and biogenesis; retrograde axon cargo transport; axon regeneration in the peripheral nervous system; synaptic transmission; axon transport of mitochondrion; regulation of axon diameter; positive regulation of axonogenesis; negative regulation of neuron apoptosis; anterograde axon cargo transport; locomotion; neuromuscular process controlling balance; neurite morphogenesis; response to corticosterone stimulus

Disease: Charcot-marie-tooth Disease, Demyelinating, Type 1f; Charcot-marie-tooth Disease, Axonal, Type 2e
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 Research Articles on NEFL    1. Data indicate that the N terminus of Pro-EMAP II binds to its C terminus, arginyl-tRNA synthetase, and the neurofilament light subunit.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with NEFL elisa kitPathways associated with NEFL elisa kit
 Reference Product  PubMed Publications
 MTMR2 elisa kit  >6 publications with NEFL and MTMR2
 GAN elisa kit  >3 publications with NEFL and GAN
 NEFH elisa kit  >3 publications with NEFL and NEFH
 NEFM elisa kit  >2 publications with NEFL and NEFM
 TSC1 elisa kit  >1 publications with NEFL and TSC1
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 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 CREB Phosphorylation Through The Activation Of CaMKII Pathway antibodies  CREB Phosphorylation Through The Activation Of CaMKII Pathway Diagram
 CREB Phosphorylation Through The Activation Of Ras Pathway antibodies  CREB Phosphorylation Through The Activation Of Ras Pathway Diagram
 Neuronal System Pathway antibodies  Neuronal System Pathway Diagram
 Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell Pathway antibodies  Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell Pathway Diagram
 Post NMDA Receptor Activation Events Pathway antibodies  Post NMDA Receptor Activation Events Pathway Diagram
 Ras Activation Uopn Ca2+ Infux Through NMDA Receptor Pathway antibodies  Ras Activation Uopn Ca2+ Infux Through NMDA Receptor Pathway Diagram
Diseases associated with NEFL elisa kitOrgans/Tissues associated with NEFL elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >55 publications with NEFL and Nervous System Diseases
 Nervous System Malformations Antibodies  >43 publications with NEFL and Nervous System Malformations
 Breast Neoplasms Antibodies  >8 publications with NEFL and Breast Neoplasms
 Brain Diseases Antibodies  >5 publications with NEFL and Brain Diseases
 Disease Models, Animal Antibodies  >4 publications with NEFL and Disease Models, Animal
 Charcot-Marie-Tooth disease, Type 1F Antibodies  >3 publications with NEFL and Charcot-Marie-Tooth disease, Type 1F
 Hypertension Antibodies  >2 publications with NEFL and Hypertension
 Lung Neoplasms Antibodies  >2 publications with NEFL and Lung Neoplasms
 Ataxia Antibodies  >2 publications with NEFL and Ataxia
 Immune System Diseases Antibodies  >2 publications with NEFL and Immune System Diseases
 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >36 publications with NEFL and Nerve
 Brain Antibodies  >18 publications with NEFL and Brain
 Prostate Antibodies  >11 publications with NEFL and Prostate
 Spinal Cord Antibodies  >8 publications with NEFL and Spinal Cord
 Lung Antibodies  >5 publications with NEFL and Lung
 Eye Antibodies  >3 publications with NEFL and Eye
 Ganglia Antibodies  >2 publications with NEFL and Ganglia
 Kidney Antibodies  >2 publications with NEFL and Kidney
 Bone Marrow Antibodies  >1 publications with NEFL and Bone Marrow
 Embryonic Tissue Antibodies  >1 publications with NEFL and Embryonic Tissue
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