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NKB elisa kit :: Human Neurokinin B ELISA Kit

Scan QR to view Datasheet Catalog #    MBS760185
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $300 +1 FREE 8GB USB
96-Strip-Wells  /  $415 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Neurokinin B (NKB), ELISA Kit

★Popular Item★
 Also Known As   

Human Neurokinin B ELISA Kit

 Product Synonym Names    NKB
 Product Gene Name   

NKB elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS760185 COA    COA PDF
 OMIM    162330
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of NKB. No significant cross-reactivity or interference between NKB and analogues is observed.
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 Samples    Serum, plasma, tissue homogenates and other biological fluids
 Assay Type    Quantitative Competitive
 Detection Range    15.625-1000pg/ml
 Sensitivity    <9.375pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NKB were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NKB were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NKB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NKB purchase    MBS760185 is a ready-to-use microwell, strip plate Quantitative Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Neurokinin B (NKB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.625-1000pg/ml in biological research samples containing NKB, with an estimated sensitivity of <9.375pg/ml. The ELISA analytical biochemical technique of the MBS760185 kit is based on NKB antibody-NKB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NKB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NKB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for NKB elisa kit

   Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with NKB. During the reaction, NKB in the sample or standard competes with a fixed amount of NKB on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to NKB. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NKB in the samples is then determined by comparing the O D of the samples to the standard curve.
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 Typical Standard Curve/Testing Data of NKB elisa kit    NKB elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS760185. Click to request current manual
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NCBI/Uniprot data below describe general gene information for NKB. It may not necessarily be applicable to this product.
 NCBI GI #    8572053
 NCBI GeneID    6866
 NCBI Accession #    AAF76980.1 [Other Products]
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 UniProt Secondary Accession #    Q6IAG2; Q71BC6; Q71BC9 [Other Products]
 UniProt Related Accession #    Q9UHF0 [Other Products]
 Molecular Weight    11,358 Da
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 NCBI Official Full Name    neurokinin B
 NCBI Official Synonym Full Names    tachykinin 3
 NCBI Official Symbol    TAC3 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    tachykinin-3
 UniProt Protein Name    Tachykinin-3
 UniProt Synonym Protein Names   
 UniProt Gene Name    TAC3 [Similar Products]
 UniProt Synonym Gene Names    NKNB; NKB [Similar Products]
 UniProt Entry Name    TKNK_HUMAN
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 NCBI Summary for NKB    This gene encodes a member of the tachykinin family of secreted neuropeptides. The encoded preproprotein is proteolytically processed to generate the mature peptide, which is primarily expressed in the central and peripheral nervous systems and functions as a neurotransmitter. This peptide is the ligand for the neurokinin-3 receptor. This protein is also expressed in the outer syncytiotrophoblast of the placenta and may be associated with pregnancy-induced hypertension and pre-eclampsia. Mutations in this gene are associated with normosmic hypogonadotropic hypogonadism. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]
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 UniProt Comments for NKB    TAC3: Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles. Belongs to the tachykinin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: extracellular region; extracellular space

Molecular Function: protein binding; receptor binding

Biological Process: female pregnancy; neuropeptide signaling pathway; tachykinin signaling pathway

Disease: Hypogonadotropic Hypogonadism 10 With Or Without Anosmia
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 Research Articles on NKB    1. Plasma neurokinin B level is higher in patients with NDHT, indicating an unfavorable cardiovascular prognosis.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with NKB elisa kitPathways associated with NKB elisa kit
 Reference Product  PubMed Publications
 TACR3 elisa kit  >44 publications with NKB and TACR3
 PROKR2 elisa kit  >22 publications with NKB and PROKR2
 KISS1R elisa kit  >21 publications with NKB and KISS1R
 PROK2 elisa kit  >19 publications with NKB and PROK2
 GNRH1 elisa kit  >15 publications with NKB and GNRH1
 TAC1 elisa kit  >8 publications with NKB and TAC1
 TACR1 elisa kit  >3 publications with NKB and TACR1
 TACR2 elisa kit  >2 publications with NKB and TACR2
 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
 Peptide Ligand-binding Receptors Pathway antibodies  Peptide Ligand-binding Receptors Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By GPCR Pathway antibodies  Signaling By GPCR Pathway Diagram
 Tachykinin Receptors Bind Tachykinins Pathway antibodies  Tachykinin Receptors Bind Tachykinins Pathway Diagram
Diseases associated with NKB elisa kitOrgans/Tissues associated with NKB elisa kit
 Disease Name  Pubmed Publications
 Hypogonadism Antibodies  >37 publications with NKB and Hypogonadism
 Congenital Abnormalities Antibodies  >12 publications with NKB and Congenital Abnormalities
 Idiopathic Hypogonadotropic Hypogonadism Antibodies  >12 publications with NKB and Idiopathic Hypogonadotropic Hypogonadism
 Nervous System Diseases Antibodies  >6 publications with NKB and Nervous System Diseases
 Neoplasm Metastasis Antibodies  >2 publications with NKB and Neoplasm Metastasis
 Gastrointestinal Diseases Antibodies  >2 publications with NKB and Gastrointestinal Diseases
 Fetal Growth Retardation Antibodies  >1 publications with NKB and Fetal Growth Retardation
 Abnormalities, Multiple Antibodies  >1 publications with NKB and Abnormalities, Multiple
 Spinal Cord Diseases Antibodies  >1 publications with NKB and Spinal Cord Diseases
 Neurobehavioral Manifestations Antibodies  >1 publications with NKB and Neurobehavioral Manifestations
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >15 publications with NKB and Brain
 Placenta Antibodies  >3 publications with NKB and Placenta
 Uterus Antibodies  >1 publications with NKB and Uterus
 Connective Tissue Antibodies  >1 publications with NKB and Connective Tissue
 Embryonic Tissue Antibodies  >1 publications with NKB and Embryonic Tissue
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