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NPYR2 elisa kit :: Human Neuropeptide Y Receptor Y2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS762919
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $300 +1 FREE 8GB USB
96-Strip-Wells  /  $415 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Neuropeptide Y Receptor Y2 (NPYR2), ELISA Kit

★Popular Item★
 Also Known As   

Human Neuropeptide Y Receptor Y2 ELISA Kit

 Product Synonym Names    NPYR2
 Product Gene Name   

NPYR2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS762919 COA    COA PDF
 OMIM    162642
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of NPYR2 . No significant cross-reactivity or interference between NPYR2 and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the crossreactivity detection between NPYR2 and all the analogues, therefore, cross reaction may still exist.
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 Samples    Serum, plasma, tissue homogenates and other biological fluids
 Assay Type    Sandwich
 Detection Range    0.313-20ng/ml
 Sensitivity    <0.188ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NPYR2 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NPYR2 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NPYR2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NPYR2 purchase    MBS762919 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Neuropeptide Y Receptor Y2 (NPYR2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.313-20ng/ml in biological research samples containing NPYR2, with an estimated sensitivity of <0.188ng/ml. The ELISA analytical biochemical technique of the MBS762919 kit is based on NPYR2 antibody-NPYR2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NPYR2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NPYR2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for NPYR2 elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- NPYR2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- NPYR2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the NPYR2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of NPYR2 can be calculated.
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 Typical Testing Data/Standard Curve (for reference only) of NPYR2 elisa kit    NPYR2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS762919. Click to request current manual
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NCBI/Uniprot data below describe general gene information for NPYR2. It may not necessarily be applicable to this product.
 NCBI GI #    29824918
 NCBI GeneID    4887
 NCBI Accession #    AAO92062.1 [Other Products]
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 UniProt Secondary Accession #    Q13281; Q13457; Q4W5G7; Q6AZZ6; Q9UE67 [Other Products]
 UniProt Related Accession #    P49146 [Other Products]
 Molecular Weight    42,731 Da
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 NCBI Official Full Name    neuropeptide Y receptor Y2
 NCBI Official Synonym Full Names    neuropeptide Y receptor Y2
 NCBI Official Symbol    NPY2R [Similar Products]
 NCBI Official Synonym Symbols   
NPY2-R
[Similar Products]
 NCBI Protein Information    neuropeptide Y receptor type 2
 UniProt Protein Name    Neuropeptide Y receptor type 2
 UniProt Synonym Protein Names   
NPY-Y2 receptor; Y2 receptor
 UniProt Gene Name    NPY2R [Similar Products]
 UniProt Synonym Gene Names    NPY2-R; Y2 receptor [Similar Products]
 UniProt Entry Name    NPY2R_HUMAN
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 UniProt Comments for NPYR2    NPY2R: Receptor for neuropeptide Y and peptide YY. The rank order of affinity of this receptor for pancreatic polypeptides is PYY > NPY > PYY (3-36) > NPY (2-36) > [Ile-31, Gln-34] PP > [Leu- 31, Pro-34] NPY > PP, [Pro-34] PYY and NPY free acid. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral; GPCR, family 1

Chromosomal Location of Human Ortholog: 4q31

Cellular Component: cytosol; integral to plasma membrane; nonmotile primary cilium; plasma membrane

Molecular Function: calcium channel regulator activity; neuropeptide Y receptor activity; peptide YY receptor activity; protein binding; receptor activity

Biological Process: behavioral fear response; cell surface receptor linked signal transduction; elevation of cytosolic calcium ion concentration; feeding behavior; G-protein signaling, adenylate cyclase inhibiting pathway; locomotory behavior; negative regulation of cAMP biosynthetic process; negative regulation of neurological process; negative regulation of secretion; negative regulation of synaptic transmission, glutamatergic; neuropeptide signaling pathway; nitric oxide mediated signal transduction; positive regulation of circadian sleep/wake cycle, non-REM sleep; positive regulation of dopamine secretion; positive regulation of peptide secretion; positive regulation of smooth muscle contraction; regulation of sensory perception of pain; secretion
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 Research Articles on NPYR2    1. Data show that the Y2-receptor high expression G allele is associated with a less efficient mode of action cascading where different task goals are activated in parallel.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with NPYR2 elisa kitPathways associated with NPYR2 elisa kit
 Reference Product  PubMed Publications
 NPY elisa kit  >20 publications with NPYR2 and NPY
 NPY1R elisa kit  >15 publications with NPYR2 and NPY1R
 NPY5R elisa kit  >13 publications with NPYR2 and NPY5R
 PYY elisa kit  >6 publications with NPYR2 and PYY
 PPY elisa kit  >2 publications with NPYR2 and PPY
 NMU elisa kit  >1 publications with NPYR2 and NMU
 POMC elisa kit  >1 publications with NPYR2 and POMC
 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class A Rhodopsin-like Pathway antibodies  GPCRs, Class A Rhodopsin-like Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
 Peptide GPCRs Pathway antibodies  Peptide GPCRs Pathway Diagram
 Peptide Ligand-binding Receptors Pathway antibodies  Peptide Ligand-binding Receptors Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
Diseases associated with NPYR2 elisa kitOrgans/Tissues associated with NPYR2 elisa kit
 Disease Name  Pubmed Publications
 Brain Diseases Antibodies  >3 publications with NPYR2 and Brain Diseases
 Abnormalities, Multiple Antibodies  >2 publications with NPYR2 and Abnormalities, Multiple
 Alcoholism Antibodies  >2 publications with NPYR2 and Alcoholism
 Hypertension Antibodies  >2 publications with NPYR2 and Hypertension
 Edema Antibodies  >1 publications with NPYR2 and Edema
 Cognition Disorders Antibodies  >1 publications with NPYR2 and Cognition Disorders
 Hypertrophy Antibodies  >1 publications with NPYR2 and Hypertrophy
 Cardiomegaly Antibodies  >1 publications with NPYR2 and Cardiomegaly
 Neurobehavioral Manifestations Antibodies  >1 publications with NPYR2 and Neurobehavioral Manifestations
 Epilepsy Antibodies  >1 publications with NPYR2 and Epilepsy
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >13 publications with NPYR2 and Brain
 Nerve Antibodies  >3 publications with NPYR2 and Nerve
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