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NRF2 elisa kit :: Human Nuclear Factor Erythroid 2-Related Factor 2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS024775 NRF2 elisa kit
Unit / Price
48-Strip-Wells  /  $500 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,250 +4 FREE 8GB USB
10x96-Strip-Wells  /  $6,395 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Nuclear Factor Erythroid 2-Related Factor 2 (NRF2), ELISA Kit

★Popular Item★
 Also Known As   

Human Nuclear Factor Erythroid 2-Related Factor 2 ELISA Kit

 Product Gene Name   

NRF2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 2; NC_000002.11 (178095031..178129859, complement). Location: 2q31
 OMIM    600492
 3D Structure    ModBase 3D Structure for Q16236
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 Species Reactivity    Human
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 Specificity    No significant cross-reactivity or interference between Human NRF2 and analogues was observed.
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 Samples    Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
 Assay Type    Sandwich
 Detection Range    62.5 pg/ml - 2000 pg/ml.
 Sensitivity    10 pg/ml.
 Intended Uses    This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated NRF2 concentrations in Human serum, plasma and other body fluids. Using Purified Human NRF2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NRF2 and NRF2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NRF2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Intra-assay Precision    Intra-assay CV (%) is less than 15%.
 Inter-assay Precision    Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NRF2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NRF2 purchase    MBS024775 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Nuclear Factor Erythroid 2-Related Factor 2 (NRF2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 62.5 pg/ml - 2000 pg/ml in biological research samples containing NRF2, with an estimated sensitivity of 10 pg/ml. The ELISA analytical biochemical technique of the MBS024775 kit is based on NRF2 antibody-NRF2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NRF2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NRF2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for NRF2 elisa kit

   Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated NRF2 concentrations in Human serum, plasma and other body fluids. Using Purified Human NRF2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NRF2 and NRF2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NRF2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS024775. Click to request current manual
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NCBI/Uniprot data below describe general gene information for NRF2. It may not necessarily be applicable to this product.
 NCBI GI #    20149576
 NCBI GeneID    4780
 NCBI Accession #    NP_006155.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_006164.4 [Other Products]
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 UniProt Primary Accession #    Q16236 [Other Products]
 UniProt Secondary Accession #    Q53RW6; Q59HH2; Q96F71; B2RBU2; B4E338; E9PGJ7 [Other Products]
 UniProt Related Accession #    Q16236 [Other Products]
 Molecular Weight    67,827 Da
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 NCBI Official Full Name    nuclear factor erythroid 2-related factor 2 isoform 1
 NCBI Official Synonym Full Names    nuclear factor (erythroid-derived 2)-like 2
 NCBI Official Symbol    NFE2L2 [Similar Products]
 NCBI Official Synonym Symbols   
NRF2
[Similar Products]
 NCBI Protein Information    nuclear factor erythroid 2-related factor 2; HEBP1; NFE2-related factor 2; NF-E2-related factor 2; nuclear factor erythroid-derived 2-like 2; nuclear factor, erythroid derived 2, like 2
 UniProt Protein Name    Nuclear factor erythroid 2-related factor 2
 UniProt Synonym Protein Names   
HEBP1; Nuclear factor, erythroid derived 2, like 2
 Protein Family    Nuclear factor erythroid 2-related factor
 UniProt Gene Name    NFE2L2 [Similar Products]
 UniProt Synonym Gene Names    NRF2; NF-E2-related factor 2 [Similar Products]
 UniProt Entry Name    NF2L2_HUMAN
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 NCBI Summary for NRF2    This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
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 UniProt Comments for NRF2    NRF2: a transcription factor that regulates basal expression and antioxidant induction of a transcription factor for NAD(P)H:quinone oxidoreductase-1 (NQO1) and other detoxifying genes. Targeted for proteasomal degradation by INrf2. Oxidative stress causes its phosphorylation and release from INrf2.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 2q31

Cellular Component: nucleoplasm; centrosome; cytoplasm; plasma membrane; cytosol; chromatin; nucleus

Molecular Function: protein domain specific binding; protein binding; DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; positive regulation of blood coagulation; unfolded protein response; regulation of embryonic development; proteasomal ubiquitin-independent protein catabolic process; protein ubiquitination; positive regulation of transcription from RNA polymerase II promoter
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 Research Articles on NRF2    1. RXRalpha diminishes cytoprotection by NRF2 by binding directly to the newly defined Neh7 domain in NRF2.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with NRF2 elisa kitPathways associated with NRF2 elisa kit
 Reference Product  PubMed Publications
 HMOX1 elisa kit  >723 publications with NRF2 and HMOX1
 KEAP1 elisa kit  >525 publications with NRF2 and KEAP1
 NQO1 elisa kit  >351 publications with NRF2 and NQO1
 JUN elisa kit  >309 publications with NRF2 and JUN
 CUL3 elisa kit  >50 publications with NRF2 and CUL3
 MAFG elisa kit  >20 publications with NRF2 and MAFG
 PMF1 elisa kit  >7 publications with NRF2 and PMF1
 CREBBP elisa kit  >4 publications with NRF2 and CREBBP
 UBC elisa kit  >2 publications with NRF2 and UBC
 Products by Pathway  Pathway Diagram
 AhR Pathway antibodies  AhR Pathway Diagram
 Keap1-Nrf2 Pathway antibodies  Keap1-Nrf2 Pathway Diagram
 Oxidative Stress Pathway antibodies  Oxidative Stress Pathway Diagram
 Protein Processing In Endoplasmic Reticulum Pathway antibodies  Protein Processing In Endoplasmic Reticulum Pathway Diagram
 Protein Processing In Endoplasmic Reticulum Pathway antibodies  Protein Processing In Endoplasmic Reticulum Pathway Diagram
 Selenium Metabolism And Selenoproteins Pathway antibodies  Selenium Metabolism And Selenoproteins Pathway Diagram
Diseases associated with NRF2 elisa kitOrgans/Tissues associated with NRF2 elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >443 publications with NRF2 and Neoplasms
 Drug Toxicity Antibodies  >408 publications with NRF2 and Drug Toxicity
 Inflammation Antibodies  >383 publications with NRF2 and Inflammation
 Disease Models, Animal Antibodies  >225 publications with NRF2 and Disease Models, Animal
 Nervous System Diseases Antibodies  >215 publications with NRF2 and Nervous System Diseases
 Necrosis Antibodies  >179 publications with NRF2 and Necrosis
 Liver Diseases Antibodies  >170 publications with NRF2 and Liver Diseases
 Brain Diseases Antibodies  >154 publications with NRF2 and Brain Diseases
 Liver Neoplasms Antibodies  >78 publications with NRF2 and Liver Neoplasms
 Gastrointestinal Diseases Antibodies  >73 publications with NRF2 and Gastrointestinal Diseases
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >399 publications with NRF2 and Liver
 Lung Antibodies  >292 publications with NRF2 and Lung
 Blood Antibodies  >289 publications with NRF2 and Blood
 Brain Antibodies  >244 publications with NRF2 and Brain
 Vascular Antibodies  >197 publications with NRF2 and Vascular
 Muscle Antibodies  >136 publications with NRF2 and Muscle
 Kidney Antibodies  >130 publications with NRF2 and Kidney
 Heart Antibodies  >122 publications with NRF2 and Heart
 Skin Antibodies  >78 publications with NRF2 and Skin
 Nerve Antibodies  >62 publications with NRF2 and Nerve
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