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TNFRSF11B elisa kit :: Human OPG ELISA Kit

Scan QR to view Datasheet Catalog #    MBS175881
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96-Strip-Wells  /  $380 +1 FREE 8GB USB
 
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 Product Name   

OPG (TNFRSF11B), ELISA Kit

★Popular Item★
 Also Known As   

Human OPG PicoKine ELISA Kit

 Product Synonym Names    Tumor necrosis factor receptor superfamily member 11B; tumor necrosis factor receptor superfamily, member 11b; MGC29565; MGC93568; OCIF; OPG; Osteoclastogenesis inhibitory factor; Osteoprotegerin; TNFRSF 11B; TNFRSF11B; TR 1; TR1; TR11B_HUMAN; Tumor necrosis factor receptor superfamily member 11B
 Product Gene Name   

TNFRSF11B elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    602643
 3D Structure    ModBase 3D Structure for O00300
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 Species Reactivity    Human
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 Specificity    Natural and recombinant Human OPG
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 Assay Type    Quantitative Sandwich
 Detection Range    93.7pg/ml-6000pg/ml
 Sensitivity    <5pg/ml
 Intra-assay Precision    Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
 Inter-assay Precision    Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
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 Preparation and Storage    Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TNFRSF11B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TNFRSF11B purchase    MBS175881 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the OPG (TNFRSF11B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 93.7pg/ml-6000pg/ml in biological research samples containing TNFRSF11B, with an estimated sensitivity of <5pg/ml. The ELISA analytical biochemical technique of the MBS175881 kit is based on TNFRSF11B antibody-TNFRSF11B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TNFRSF11B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TNFRSF11B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TNFRSF11B elisa kit

   Principle of the Assay: Human OPG ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for OPG has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: E22-L401) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for OPG is added subsequently and then followed by washing with PBS or TBS buffer. Avidin- Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human OPG amount of sample captured in plate.

Background: Osteoprotegerin(OPG) is identical to osteoclastogenesis inhibitory factor(OCIF), a soluble member of the tumor-necrosis factor receptor family that inhibits osteoclastogenesis. OPG is considered to play an important role in the regulation of bone resorption by modifying osteoclast differentiation. Osteoprotegerin is an independent risk factor for the progression of atherosclerosis and onset of cardiovascular disease. It can act as a soluble factor in the regulation of bone mass and imply a utility for OPG in the treatment of osteoporosis associated with increased osteoclast activity. OPG system may play a critical role in the development of osteolytic bone disease in multiple myeloma and that targeting this system may have therapeutic potential. OPG also plays a role in the vascular system. The standard product used in this kit is recombinant OPG, a 55KDa glycoprotein.
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 Typical Testing Data/Standard Curve (for reference only) of TNFRSF11B elisa kit    TNFRSF11B elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS175881. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TNFRSF11B. It may not necessarily be applicable to this product.
 NCBI GI #    322510101
 NCBI GeneID    4982
 NCBI Accession #    O00300.3 [Other Products]
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 UniProt Primary Accession #    O00300 [Other Products]
 UniProt Secondary Accession #    O60236; Q53FX6; Q9UHP4; B2R9A8 [Other Products]
 UniProt Related Accession #    O00300 [Other Products]
 Molecular Weight    46,026 Da
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 NCBI Official Full Name    Tumor necrosis factor receptor superfamily member 11B
 NCBI Official Synonym Full Names    tumor necrosis factor receptor superfamily, member 11b
 NCBI Official Symbol    TNFRSF11B [Similar Products]
 NCBI Official Synonym Symbols   
OPG; TR1; OCIF
[Similar Products]
 NCBI Protein Information    tumor necrosis factor receptor superfamily member 11B; osteoprotegerin; osteoclastogenesis inhibitory factor
 UniProt Protein Name    Tumor necrosis factor receptor superfamily member 11B
 UniProt Synonym Protein Names   
Osteoclastogenesis inhibitory factor; Osteoprotegerin
 Protein Family    Tumor necrosis factor receptor superfamily
 UniProt Gene Name    TNFRSF11B [Similar Products]
 UniProt Synonym Gene Names    OCIF; OPG [Similar Products]
 UniProt Entry Name    TR11B_HUMAN
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 NCBI Summary for TNFRSF11B    The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined. [provided by RefSeq, Jul 2008]
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 UniProt Comments for TNFRSF11B    TNFRSF11B: Acts as decoy receptor for RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local RANKL/OPG ratio. May also play a role in preventing arterial calcification. May act as decoy receptor for TRAIL and protect against apoptosis. TRAIL binding blocks the inhibition of osteoclastogenesis. Defects in TNFRSF11B are the cause of juvenile Paget disease (JPD); also known as hyperostosis corticalis deformans juvenilis or hereditary hyperphosphatasia or chronic congenital idiopathic hyperphosphatasia. JPD is a rare autosomal recessive osteopathy that presents in infancy or early childhood. The disorder is characterized by rapidly remodeling woven bone, osteopenia, debilitating fractures, and deformities due to a markedly accelerated rate of bone remodeling throughout the skeleton. Approximately 40 cases of JPD have been reported worldwide. Unless it is treated with drugs that block osteoclast- mediated skeletal resorption, the disease can be fatal.

Protein type: Secreted, signal peptide; Inhibitor; Secreted

Chromosomal Location of Human Ortholog: 8q24

Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function: cytokine activity; receptor activity

Biological Process: response to drug; extracellular matrix organization and biogenesis; response to magnesium ion; apoptosis; negative regulation of odontogenesis of dentine-containing teeth; response to estrogen stimulus; response to arsenic; signal transduction; negative regulation of bone resorption; skeletal development; response to nutrient

Disease: Paget Disease, Juvenile
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 Research Articles on TNFRSF11B    1. study to evaluate frequency of alleles in TNFRSF11B gene rs2073617, rs2073618 and rs3134069 polymorphisms in patients with diabetic foot, diabetes and controls; confirmed correlation between rs2073618 in the TNFRSF11B gene and diabetic foot in patients with diabetes
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TNFRSF11B elisa kitPathways associated with TNFRSF11B elisa kit
 Reference Product  PubMed Publications
 TNFSF11 elisa kit  >2278 publications with TNFRSF11B and TNFSF11
 TNFSF10 elisa kit  >171 publications with TNFRSF11B and TNFSF10
 TGFB1 elisa kit  >66 publications with TNFRSF11B and TGFB1
 IL17A elisa kit  >36 publications with TNFRSF11B and IL17A
 PPARG elisa kit  >30 publications with TNFRSF11B and PPARG
 IL18 elisa kit  >14 publications with TNFRSF11B and IL18
 IGF1R elisa kit  >7 publications with TNFRSF11B and IGF1R
 BMPR2 elisa kit  >5 publications with TNFRSF11B and BMPR2
 PRL elisa kit  >5 publications with TNFRSF11B and PRL
 Products by Pathway  Pathway Diagram
 Apoptosis Modulation And Signaling Pathway antibodies  Apoptosis Modulation And Signaling Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Monoamine Transport Pathway antibodies  Monoamine Transport Pathway Diagram
 Osteoblast Signaling Pathway antibodies  Osteoblast Signaling Pathway Diagram
 Osteoclast Signaling Pathway antibodies  Osteoclast Signaling Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram
 RANKL/RANK Signaling Pathway antibodies  RANKL/RANK Signaling Pathway Diagram
Diseases associated with TNFRSF11B elisa kitOrgans/Tissues associated with TNFRSF11B elisa kit
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >1759 publications with TNFRSF11B and Necrosis
 Bone Resorption Antibodies  >1705 publications with TNFRSF11B and Bone Resorption
 Osteoporosis Antibodies  >896 publications with TNFRSF11B and Osteoporosis
 Cardiovascular Diseases Antibodies  >601 publications with TNFRSF11B and Cardiovascular Diseases
 Inflammation Antibodies  >503 publications with TNFRSF11B and Inflammation
 Arthritis, Rheumatoid Antibodies  >285 publications with TNFRSF11B and Arthritis, Rheumatoid
 Osteolysis Antibodies  >246 publications with TNFRSF11B and Osteolysis
 Kidney Diseases Antibodies  >202 publications with TNFRSF11B and Kidney Diseases
 Heart Diseases Antibodies  >200 publications with TNFRSF11B and Heart Diseases
 Nervous System Diseases Antibodies  >125 publications with TNFRSF11B and Nervous System Diseases
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >3667 publications with TNFRSF11B and Bone
 Connective Tissue Antibodies  >708 publications with TNFRSF11B and Connective Tissue
 Bone Marrow Antibodies  >669 publications with TNFRSF11B and Bone Marrow
 Vascular Antibodies  >599 publications with TNFRSF11B and Vascular
 Mouth Antibodies  >285 publications with TNFRSF11B and Mouth
 Kidney Antibodies  >210 publications with TNFRSF11B and Kidney
 Heart Antibodies  >160 publications with TNFRSF11B and Heart
 Muscle Antibodies  >160 publications with TNFRSF11B and Muscle
 Liver Antibodies  >71 publications with TNFRSF11B and Liver
 Brain Antibodies  >67 publications with TNFRSF11B and Brain
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