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OSF elisa kit :: Human OSF ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2504620
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   


★Popular Item★
 Also Known As   

Human OSF (Osteoclast Stimulating Factor 1) ELISA Kit

 Product Gene Name   

OSF elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    610180
 3D Structure    ModBase 3D Structure for Q92882
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 Species Reactivity    Human
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 Specificity    This kit recognizes natural and recombinant Human OSF. No significant cross-reactivity or interference between Human OSF and analogues was observed.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    31.25-2000pg/mL
 Sensitivity    Min: 18.75pg/mL; Max: 2000pg/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of OSF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for OSF purchase    MBS2504620 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the OSF, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25-2000pg/mL in biological research samples containing OSF, with an estimated sensitivity of Min: 18.75pg/mL; Max: 2000pg/mL. The ELISA analytical biochemical technique of the MBS2504620 kit is based on OSF antibody-OSF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect OSF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, OSF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for OSF elisa kit

   Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human OSF concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to OSF. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for OSF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain OSF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of OSF. You can calculate the concentration of OSF in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of OSF elisa kit    OSF elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2504620. Click to request current manual
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NCBI/Uniprot data below describe general gene information for OSF. It may not necessarily be applicable to this product.
 NCBI GI #    166235148
 NCBI GeneID    26578
 NCBI Accession #    NP_036515.4 [Other Products]
 NCBI GenBank Nucleotide #    NM_012383.4 [Other Products]
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 UniProt Primary Accession #    Q92882 [Other Products]
 UniProt Secondary Accession #    Q5W126; Q96IJ4 [Other Products]
 UniProt Related Accession #    Q92882 [Other Products]
 Molecular Weight    23,787 Da
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 NCBI Official Full Name    osteoclast-stimulating factor 1
 NCBI Official Synonym Full Names    osteoclast stimulating factor 1
 NCBI Official Symbol    OSTF1 [Similar Products]
 NCBI Official Synonym Symbols   
OSF; SH3P2; bA235O14.1
[Similar Products]
 NCBI Protein Information    osteoclast-stimulating factor 1
 UniProt Protein Name    Osteoclast-stimulating factor 1
 UniProt Gene Name    OSTF1 [Similar Products]
 UniProt Entry Name    OSTF1_HUMAN
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 NCBI Summary for OSF    Osteoclast-stimulating factor-1 is an intracellular protein produced by osteoclasts that indirectly induces osteoclast formation and bone resorption (Reddy et al., 1998 [PubMed 10092216]).[supplied by OMIM, Mar 2008]
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 UniProt Comments for OSF    OSTF1: Induces bone resorption, acting probably through a signaling cascade which results in the secretion of factor(s) enhancing osteoclast formation and activity.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 9q13-q21.2

Cellular Component: cytoplasm; intracellular

Molecular Function: protein binding; SH3 domain binding

Biological Process: ossification; signal transduction
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 Research Articles on OSF    1. Data show that SH3P2 was phosphorylated on Ser(202) by ribosomal S6 kinase (RSK) in an ERK pathway-dependent manner, and such phosphorylation inhibited the ability of SH3P2 to suppress cell motility.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with OSF elisa kitDiseases associated with OSF elisa kit
 Reference Product  PubMed Publications
 KHDRBS1 elisa kit  >1 publications with OSF and KHDRBS1
 CBL elisa kit  >1 publications with OSF and CBL
 Disease Name  Pubmed Publications
 Immune System Diseases Antibodies  >3 publications with OSF and Immune System Diseases
 Obesity Antibodies  >2 publications with OSF and Obesity
 Inflammation Antibodies  >2 publications with OSF and Inflammation
 Necrosis Antibodies  >2 publications with OSF and Necrosis
 Neoplasm Metastasis Antibodies  >1 publications with OSF and Neoplasm Metastasis
 Infertility, Male Antibodies  >1 publications with OSF and Infertility, Male
 Kidney Diseases Antibodies  >1 publications with OSF and Kidney Diseases
Organs/Tissues associated with OSF elisa kit
 Organ/Tissue Name  Pubmed Publications
 Bone Marrow Antibodies  >9 publications with OSF and Bone Marrow
 Blood Antibodies  >7 publications with OSF and Blood
 Connective Tissue Antibodies  >4 publications with OSF and Connective Tissue
 Kidney Antibodies  >4 publications with OSF and Kidney
 Brain Antibodies  >3 publications with OSF and Brain
 Nerve Antibodies  >2 publications with OSF and Nerve
 Intestine Antibodies  >2 publications with OSF and Intestine
 Heart Antibodies  >1 publications with OSF and Heart
 Lung Antibodies  >1 publications with OSF and Lung
 Testis Antibodies  >1 publications with OSF and Testis
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