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OCLN elisa kit :: Human Occludin (OCLN) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2704294 (SPECIAL PROMOTIONAL PRICING for a limited time)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $220 +1 FREE 8GB USB
48-Strip-Wells  /  $355 +1 FREE 8GB USB
96-Strip-Wells  /  $475 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,995 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,695 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Occludin (OCLN), ELISA Kit

★Popular Item★
 Also Known As   

Human Occludin (OCLN) ELISA Kit

 Product Synonym Names    Tight Junction Protein Occludin TM4 Minus
 Product Gene Name   

OCLN elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    U49184 mRNA
 3D Structure    ModBase 3D Structure for Q16625
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Occludin (OCLN).
No significant cross-reactivity or interference between Occludin (OCLN) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Tissue Homogenates, Cell Lysates and Other Biological Fluids
 Detection Range    0.156-10ng/mL
 Sensitivity    < 0.055ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Occludin (OCLN) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Occludin (OCLN) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of OCLN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for OCLN purchase    MBS2704294 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Occludin (OCLN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing OCLN, with an estimated sensitivity of < 0.055ng/mL. The ELISA analytical biochemical technique of the MBS2704294 kit is based on OCLN antibody-OCLN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect OCLN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, OCLN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue Homogenates, Cell Lysates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for OCLN elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Occludin (OCLN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Occludin (OCLN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Occludin (OCLN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Occludin (OCLN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of OCLN elisa kit    OCLN elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2704294. Click to request current manual
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NCBI/Uniprot data below describe general gene information for OCLN. It may not necessarily be applicable to this product.
 NCBI GI #    1322282
 NCBI GeneID    100506658
 NCBI Accession #    AAB00195.1 [Other Products]
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 UniProt Primary Accession #    Q16625 [Other Products]
 UniProt Secondary Accession #    Q5U1V4; Q8N6K1; B5BU70; D2DU64; D2DU65; D2IGC0; D2IGC1; E2CYV9 [Other Products]
 UniProt Related Accession #    Q16625 [Other Products]
 Molecular Weight    8,175 Da
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 NCBI Official Full Name    occludin
 NCBI Official Synonym Full Names    occludin
 NCBI Official Symbol    OCLN [Similar Products]
 NCBI Official Synonym Symbols   
BLCPMG; PTORCH1; PPP1R115
[Similar Products]
 NCBI Protein Information    occludin
 UniProt Protein Name    Occludin
 Protein Family    Occludin
 UniProt Gene Name    OCLN [Similar Products]
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 NCBI Summary for OCLN    This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. [provided by RefSeq, Apr 2011]
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 UniProt Comments for OCLN    May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.
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 Research Articles on OCLN    1. Glutamine increased claudin-1 expression in the colonic mucosa of patients with irritable bowel syndrome. In contrast, occludin expression was not significantly modified by glutamine.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with OCLN elisa kitPathways associated with OCLN elisa kit
 Reference Product  PubMed Publications
 TJP1 elisa kit  >525 publications with OCLN and TJP1
 CLDN1 elisa kit  >273 publications with OCLN and CLDN1
 CLDN5 elisa kit  >128 publications with OCLN and CLDN5
 CLDN4 elisa kit  >63 publications with OCLN and CLDN4
 TJP2 elisa kit  >48 publications with OCLN and TJP2
 CLDN3 elisa kit  >47 publications with OCLN and CLDN3
 CLDN7 elisa kit  >17 publications with OCLN and CLDN7
 TJP3 elisa kit  >13 publications with OCLN and TJP3
 CGN elisa kit  >7 publications with OCLN and CGN
 YES1 elisa kit  >1 publications with OCLN and YES1
 Products by Pathway  Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Pathogenic Escherichia Coli Infection Pathway antibodies  Pathogenic Escherichia Coli Infection Pathway Diagram
 Pathogenic Escherichia Coli Infection Pathway antibodies  Pathogenic Escherichia Coli Infection Pathway Diagram
 Pathogenic Escherichia Coli Infection Pathway antibodies  Pathogenic Escherichia Coli Infection Pathway Diagram
 TGF-beta Receptor Signaling Pathway antibodies  TGF-beta Receptor Signaling Pathway Diagram
Diseases associated with OCLN elisa kitOrgans/Tissues associated with OCLN elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >140 publications with OCLN and Inflammation
 Disease Models, Animal Antibodies  >113 publications with OCLN and Disease Models, Animal
 Necrosis Antibodies  >81 publications with OCLN and Necrosis
 Cardiovascular Diseases Antibodies  >78 publications with OCLN and Cardiovascular Diseases
 Edema Antibodies  >59 publications with OCLN and Edema
 Liver Diseases Antibodies  >42 publications with OCLN and Liver Diseases
 Retinal Diseases Antibodies  >32 publications with OCLN and Retinal Diseases
 Colitis Antibodies  >28 publications with OCLN and Colitis
 Kidney Diseases Antibodies  >15 publications with OCLN and Kidney Diseases
 Hemorrhage Antibodies  >13 publications with OCLN and Hemorrhage
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >263 publications with OCLN and Brain
 Intestine Antibodies  >229 publications with OCLN and Intestine
 Liver Antibodies  >120 publications with OCLN and Liver
 Eye Antibodies  >100 publications with OCLN and Eye
 Kidney Antibodies  >70 publications with OCLN and Kidney
 Testis Antibodies  >55 publications with OCLN and Testis
 Embryonic Tissue Antibodies  >51 publications with OCLN and Embryonic Tissue
 Lung Antibodies  >46 publications with OCLN and Lung
 Stomach Antibodies  >19 publications with OCLN and Stomach
 Pancreas Antibodies  >15 publications with OCLN and Pancreas
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