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Add To Compare List OCLN elisa kit :: Human Occludin ELISA Kit Catalog # MBS727773 Unit / Price 48-Strip-Wells-(Competitive) / $495 +1 FREE 8GB USB 48-Strip-Wells-(Sandwich) / $495 +1 FREE 8GB USB 96-Strip-Wells-(Competitive) / $700 +1 FREE 8GB USB 96-Strip-Wells-(Sandwich) / $700 +1 FREE 8GB USB 5x96-Strip-Wells-(Competitive) / $3,105 +3 FREE 8GB USB 5x96-Strip-Wells-(Sandwich) / $3,105 +3 FREE 8GB USB 10x96-Strip-Wells-(Competitive) / $5,865 +7 FREE 8GB USB 10x96-Strip-Wells-(Sandwich) / $5,865 +7 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Occludin (OCLN), ELISA Kit Popular Item Full Product Name Human Occludin ELISA Kit Product Gene Name OCLN elisa kit[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sandwich ELISA Manual Insert Sample Sandwich PDF Manual OMIM 602876 Species Reactivity Human Specificity This assay has high sensitivity and excellent specificity for detection of OCLN. No significant cross-reactivity or interference between OCLN and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between OCLN and all the analogues, therefore, cross reaction may still exist in some cases. Samples Serum, plasma, cell culture supernatants, body fluid and tissue homogenate Assay Type Quantitative Competitive or Sandwich Sensitivity 0.1 ng/mL Preparation and Storage Store all reagents at 2-8 degree C. Sample Preparation We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team ISO Certification Manufactured in an ISO 13485:2016 Certified Laboratory. Product Note Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details. Other Notes Small volumes of OCLN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. Searchable Terms for OCLN purchase MBS727773 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Occludin (OCLN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing OCLN. The ELISA analytical biochemical technique of the MBS727773 kit is based on OCLN antibody-OCLN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect OCLN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, OCLN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). TOP Related Product Information for OCLN elisa kit Intended Uses: This OCLN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human OCLN. This ELISA kit for research use only, not for therapeutic or test applications!Principle of the Assay: OCLN ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for OCLN. Standards or samples are then added to the microtiter plate wells and OCLN if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of OCLN present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for OCLN are added to each well to "sandwich" the OCLN immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain OCLN and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OCLN concentration in each sample is interpolated from this standard curve. Product Categories/Family for OCLN elisa kit Signal Transduction Typical Testing Data/Standard Curve (for reference only) of OCLN elisa kit TOP NCBI/Uniprot data below describe general gene information for OCLN. It may not necessarily be applicable to this product. NCBI GI # 1322282 NCBI GeneID 100506658 NCBI Accession # AAB00195.1 [Other Products] UniProt Secondary Accession # Q5U1V4; Q8N6K1; B5BU70; D2DU64; D2DU65; D2IGC0; D2IGC1; E2CYV9[Other Products] UniProt Related Accession # Q16625[Other Products] Molecular Weight 8,175 Da TOP NCBI Official Full Name occludin NCBI Official Synonym Full Names occludin NCBI Official Symbol OCLN [Similar Products] NCBI Official Synonym Symbols BLCPMG; PPP1R115 [Similar Products] NCBI Protein Information occludin; tight junction protein occludin; phosphatase 1, regulatory subunit 115 UniProt Protein Name Occludin Protein Family Occludin UniProt Gene Name OCLN [Similar Products] UniProt Entry Name OCLN_HUMAN TOP NCBI Summary for OCLN This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. [provided by RefSeq, Apr 2011] TOP UniProt Comments for OCLN occludin: May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. Interacts with TJP1/ZO1 and with VAPA. Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis. Belongs to the ELL/occludin family. 7 isoforms of the human protein are produced by alternative splicing.Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Membrane protein, multi-pass; Cell adhesionChromosomal Location of Human Ortholog: 5q13.1Cellular Component: tight junction; apicolateral plasma membrane; endocytic vesicle; apical plasma membrane; plasma membrane; integral to membrane; intercellular junction; cytoplasmic vesicle; cytosol; cell junctionMolecular Function: thiopurine S-methyltransferase activity; protein domain specific binding; protein binding; structural molecule activityBiological Process: methylation; intercellular junction assembly and maintenance; apoptosis; S-adenosylmethionine metabolic process; protein complex assembly; S-adenosylhomocysteine metabolic process; cell structure disassembly during apoptosisDisease: Band-like Calcification With Simplified Gyration And Polymicrogyria Research Articles on OCLN 1. findings suggest that cerebral vascular cells express functional BACE1. Moreover, elevated vascular BACE1 may contribute to deficiency of occludin in caa. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP Products associated with OCLN elisa kit Reference Product PubMed Publications TJP1 elisa kit >525 publications with OCLN and TJP1 CLDN1 elisa kit >273 publications with OCLN and CLDN1 CLDN5 elisa kit >128 publications with OCLN and CLDN5 CLDN4 elisa kit >63 publications with OCLN and CLDN4 TJP2 elisa kit >48 publications with OCLN and TJP2 CLDN3 elisa kit >47 publications with OCLN and CLDN3 CLDN7 elisa kit >17 publications with OCLN and CLDN7 TJP3 elisa kit >13 publications with OCLN and TJP3 CGN elisa kit >7 publications with OCLN and CGN YES1 elisa kit >1 publications with OCLN and YES1 Pathways associated with OCLN elisa kit Products by Pathway Pathway Diagram Cell Adhesion Molecules (CAMs) Pathway antibodies Cell Adhesion Molecules (CAMs) Pathway Diagram Cell Adhesion Molecules (CAMs) Pathway antibodies Cell Adhesion Molecules (CAMs) Pathway Diagram Hepatitis C Pathway antibodies Hepatitis C Pathway Diagram Hepatitis C Pathway antibodies Hepatitis C Pathway Diagram Leukocyte Transendothelial Migration Pathway antibodies Leukocyte Transendothelial Migration Pathway Diagram Leukocyte Transendothelial Migration Pathway antibodies Leukocyte Transendothelial Migration Pathway Diagram Pathogenic Escherichia Coli Infection Pathway antibodies Pathogenic Escherichia Coli Infection Pathway Diagram Pathogenic Escherichia Coli Infection Pathway antibodies Pathogenic Escherichia Coli Infection Pathway Diagram Pathogenic Escherichia Coli Infection Pathway antibodies Pathogenic Escherichia Coli Infection Pathway Diagram TGF-beta Receptor Signaling Pathway antibodies TGF-beta Receptor Signaling Pathway Diagram Diseases associated with OCLN elisa kit Disease Name Pubmed Publications Inflammation Antibodies >140 publications with OCLN and Inflammation Disease Models, Animal Antibodies >113 publications with OCLN and Disease Models, Animal Necrosis Antibodies >81 publications with OCLN and Necrosis Cardiovascular Diseases Antibodies >78 publications with OCLN and Cardiovascular Diseases Edema Antibodies >59 publications with OCLN and Edema Liver Diseases Antibodies >42 publications with OCLN and Liver Diseases Retinal Diseases Antibodies >32 publications with OCLN and Retinal Diseases Colitis Antibodies >28 publications with OCLN and Colitis Kidney Diseases Antibodies >15 publications with OCLN and Kidney Diseases Hemorrhage Antibodies >13 publications with OCLN and Hemorrhage Organs/Tissues associated with OCLN elisa kit Organ/Tissue Name Pubmed Publications Brain Antibodies >263 publications with OCLN and Brain Intestine Antibodies >229 publications with OCLN and Intestine Liver Antibodies >120 publications with OCLN and Liver Eye Antibodies >100 publications with OCLN and Eye Kidney Antibodies >70 publications with OCLN and Kidney Testis Antibodies >55 publications with OCLN and Testis Embryonic Tissue Antibodies >51 publications with OCLN and Embryonic Tissue Lung Antibodies >46 publications with OCLN and Lung Stomach Antibodies >19 publications with OCLN and Stomach Pancreas Antibodies >15 publications with OCLN and Pancreas TOP © 2006-24 MyBioSource.com. 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