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OC elisa kit :: Human Osteocalcin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS721862
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells-(Competitive)  /  $455 +1 FREE 8GB USB
48-Strip-Wells-(Sandwich)  /  $455 +1 FREE 8GB USB
96-Strip-Wells-(Competitive)  /  $660 +1 FREE 8GB USB
96-Strip-Wells-(Sandwich)  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells-(Competitive)  /  $2,925 +3 FREE 8GB USB
5x96-Strip-Wells-(Sandwich)  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells-(Competitive)  /  $5,490 +6 FREE 8GB USB
10x96-Strip-Wells-(Sandwich)  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Osteocalcin, ELISA Kit

★Popular Item★
 Also Known As   

Human Osteocalcin ELISA Kit

 Product Gene Name   

OC elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sandwich ELISA Manual Insert    Sandwich ELISA PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    112260
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of OC. No significant cross-reactivity or interference between OC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between OC and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive or Sandwich
 Detection Range    5.0-100ng/mL
 Sensitivity    1.0ng/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of OC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for OC purchase    MBS721862 is a ready-to-use microwell, strip plate Competitive or Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Osteocalcin, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 5.0-100ng/mL in biological research samples containing OC, with an estimated sensitivity of 1.0ng/mL. The ELISA analytical biochemical technique of the MBS721862 kit is based on OC antibody-OC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect OC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, OC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for OC elisa kit

   Intended Uses: This OC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human OC. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: OC ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for OC. Standards or samples are then added to the microtiter plate wells and OC if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of OC present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for OC are added to each well to "sandwich" the OC immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain OC and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OC concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for OC elisa kit    Signal Transduction
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 Typical Standard Curve/Testing Data of OC elisa kit    OC elisa kit Typical Standard Curve/Testing Data image
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NCBI/Uniprot data below describe general gene information for OC. It may not necessarily be applicable to this product.
 NCBI GI #    40316933
 NCBI GeneID    632
 NCBI Accession #    NP_954642.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_199173.4 [Other Products]
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 UniProt Secondary Accession #    Q5TCK6 [Other Products]
 UniProt Related Accession #    P02818 [Other Products]
 Molecular Weight    10,963 Da
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 NCBI Official Full Name    osteocalcin preproprotein
 NCBI Official Synonym Full Names    bone gamma-carboxyglutamate (gla) protein
 NCBI Official Symbol    BGLAP [Similar Products]
 NCBI Official Synonym Symbols   
OC; BGP; OCN
[Similar Products]
 NCBI Protein Information    osteocalcin; bone Gla protein; gamma-carboxyglutamic acid-containing protein; bone gamma-carboxyglutamate (gla) protein (osteocalcin)
 UniProt Protein Name    Osteocalcin
 UniProt Synonym Protein Names   
Bone Gla protein; BGP; Gamma-carboxyglutamic acid-containing protein
 Protein Family    Osteocalcin
 UniProt Gene Name    BGLAP [Similar Products]
 UniProt Synonym Gene Names    BGP [Similar Products]
 UniProt Entry Name    OSTCN_HUMAN
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 NCBI Summary for OC    This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product. [provided by RefSeq, Jun 2015]
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 UniProt Comments for OC    osteocalcin: Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Belongs to the osteocalcin/matrix Gla protein family.

Protein type: Secreted; Secreted, signal peptide; Cell development/differentiation

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: Golgi apparatus; extracellular space; rough endoplasmic reticulum; dendrite; cytoplasm; perikaryon

Molecular Function: structural constituent of bone; hydroxyapatite binding; calcium ion binding; structural molecule activity

Biological Process: response to drug; response to gravity; regulation of bone resorption; response to glucocorticoid stimulus; cell aging; response to testosterone stimulus; bone mineralization; osteoblast development; osteoblast differentiation; odontogenesis; response to vitamin D; regulation of osteoclast differentiation; response to ethanol; response to vitamin K; response to zinc ion; response to estrogen stimulus; response to mechanical stimulus; response to hydroxyisoflavone; response to activity; cell adhesion; skeletal development; regulation of bone mineralization
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 Research Articles on OC    1. Serum osteocalcin is associated with indices of obesity and HDL level in men
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with OC elisa kitPathways associated with OC elisa kit
 Reference Product  PubMed Publications
 RUNX2 elisa kit  >35 publications with OC and RUNX2
 BMP2 elisa kit  >12 publications with OC and BMP2
 VDR elisa kit  >4 publications with OC and VDR
 TGFB1 elisa kit  >3 publications with OC and TGFB1
 PTH elisa kit  >2 publications with OC and PTH
 LEP elisa kit  >1 publications with OC and LEP
 PTHLH elisa kit  >1 publications with OC and PTHLH
 ATF4 elisa kit  >1 publications with OC and ATF4
 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 FGF Signaling Pathway antibodies  FGF Signaling Pathway Diagram
 Glucocorticoid Receptor Regulatory Network Pathway antibodies  Glucocorticoid Receptor Regulatory Network Pathway Diagram
 Interleukin-11 Signaling Pathway antibodies  Interleukin-11 Signaling Pathway Diagram
 M Phase Pathway antibodies  M Phase Pathway Diagram
 Mitotic Anaphase Pathway antibodies  Mitotic Anaphase Pathway Diagram
 Mitotic Metaphase And Anaphase Pathway antibodies  Mitotic Metaphase And Anaphase Pathway Diagram
 Mitotic Prometaphase Pathway antibodies  Mitotic Prometaphase Pathway Diagram
 Notch-mediated HES/HEY Network Pathway antibodies  Notch-mediated HES/HEY Network Pathway Diagram
Diseases associated with OC elisa kitOrgans/Tissues associated with OC elisa kit
 Disease Name  Pubmed Publications
 Bone Diseases Antibodies  >9 publications with OC and Bone Diseases
 Osteoporosis Antibodies  >8 publications with OC and Osteoporosis
 Inflammation Antibodies  >4 publications with OC and Inflammation
 Nervous System Diseases Antibodies  >4 publications with OC and Nervous System Diseases
 Kidney Diseases Antibodies  >2 publications with OC and Kidney Diseases
 Weight Loss Antibodies  >1 publications with OC and Weight Loss
 Renal Insufficiency Antibodies  >1 publications with OC and Renal Insufficiency
 Hypertension Antibodies  >1 publications with OC and Hypertension
 Cardiovascular Diseases Antibodies  >1 publications with OC and Cardiovascular Diseases
 Necrosis Antibodies  >1 publications with OC and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >76 publications with OC and Bone
 Brain Antibodies  >3 publications with OC and Brain
 Parathyroid Antibodies  >2 publications with OC and Parathyroid
 Prostate Antibodies  >1 publications with OC and Prostate
 Lung Antibodies  >1 publications with OC and Lung
 Heart Antibodies  >1 publications with OC and Heart
 Intestine Antibodies  >1 publications with OC and Intestine
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