Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow SELP rightarrow Human SELP  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • AICDA Antibody
 • NUP85 ELISA Kit
 • KLRD1 Antibody
 • CD28 Antibody
 • C Reactive Protein (CRP) ELISA Kit
 • Vascular endothelial growth factor C (Vegfc) Recombinant Protein
 • Lactate Dehydrogenase 3 (LDH-3) Protein
 • GPR39 Antibody
 • hnRNP C1-2 (Phospho-Ser260) Antibody
 • Gentamycin sulfate, 50MG-ml solution Biochemical
 • 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase be... Recombinant Protein
 • BCL-XL (Ab-115) Antibody
 • O-acetyl-ADP-ribose deacetylase MACROD2 (macrod2) Recombinant Protein
 • Octamer Binding Transcription Factor 4 (OBTF4) ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

SELP elisa kit :: Human P-Selectin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS176001
 Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96-Strip-Wells  /  $360 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

P-Selectin (SELP), ELISA Kit

★Popular Item★
 Also Known As   

Human P-Selectin PicoKine ELISA Kit

 Product Synonym Names    P-selectin; selectin P (granule membrane protein 140kDa, antigen CD62); Antigen CD62; CD62; CD62 antigen like family member P; CD62 antigen-like family member P; CD62P; FLJ45155; GMP 140; GMP-140; GMP140; Granule membrane protein 140; Granule membrane protein 140kDa; Granulocyte membrane protein; GRMP; LECAM3; Leukocyte endothelial cell adhesion molecule 3; Leukocyte-endothelial cell adhesion molecule 3; LYAM3_HUMAN; P selectin; P-selectin; PADGEM; Platelet activation dependent granule external membrane protein; Platelet activation dependent granule-external membrane protein; Platelet alpha granule membrane protein; PSEL; Selectin P; Selectin P (granule membrane protein 140kDa antigen CD62); SELP
 Product Gene Name   

SELP elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    gene+601367
 3D Structure    ModBase 3D Structure for P16109
Section Bar
 Species Reactivity    Human
Section Bar
 Specificity    Natural and recombinant Human P-Selectin
Section Bar
 Assay Type    Quantitative Sandwich
 Detection Range    156pg/ml-10000pg/ml
 Sensitivity    <5pg/ml
Section Bar
 Preparation and Storage    Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of SELP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for SELP purchase    MBS176001 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the P-Selectin (SELP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 156pg/ml-10000pg/ml in biological research samples containing SELP, with an estimated sensitivity of <5pg/ml. The ELISA analytical biochemical technique of the MBS176001 kit is based on SELP antibody-SELP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SELP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SELP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for SELP elisa kit

   Principle of the Assay: Human P-Selectin ELISA Kit was based on standard sandwich enzyme-linked immunesorbent assay technology. A monoclonal antibody from mouse specific for P-Selectin has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: W42-A771) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for P-Selectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human P-Selectin amount of sample captured in plate.

Background: P-selectin, also called GMP-140, CD62, or selectin P, is a 140-kD adhesion molecule, expressed at the surface of activated cells, that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is stored in secretory granules and expressed at the plasma membrane after cell activation. It is known to play an important role in atherosclerosis. The major ligand for P-selectin on leukocytes is P-selectin glycoprotein ligand-1(PSGL-1). The standard product used in this kit is recombinant human P-Selectin, excluding intercellular P-Selectin and transmembrane domain. It has 730 amino acids sequence with the molecular mass of 80 KDa.
Section Bar
  Typical Testing Data/Standard Curve (for reference only) of SELP elisa kit    SELP elisa kit  Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS176001. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for SELP. It may not necessarily be applicable to this product.
 NCBI GI #    215274139
 NCBI GeneID    6403
 NCBI Accession #    P16109.3 [Other Products]
Section Bar
 UniProt Primary Accession #    P16109 [Other Products]
 UniProt Secondary Accession #    Q5R344; Q8IVD1 [Other Products]
 UniProt Related Accession #    P16109 [Other Products]
 Molecular Weight    90,834 Da
Table BarTOPTable Bar
 NCBI Official Full Name    P-selectin
 NCBI Official Synonym Full Names    selectin P (granule membrane protein 140kDa, antigen CD62)
 NCBI Official Symbol    SELP [Similar Products]
 NCBI Official Synonym Symbols   
CD62; GRMP; PSEL; CD62P; GMP140; LECAM3; PADGEM
[Similar Products]
 NCBI Protein Information    P-selectin; GMP-140; granule membrane protein 140; granulocyte membrane protein; CD62 antigen-like family member P; platelet alpha-granule membrane protein; leukocyte-endothelial cell adhesion molecule 3; platelet activation dependent granule-external membrane protein
 UniProt Protein Name    P-selectin
 UniProt Synonym Protein Names   
CD62 antigen-like family member P; Granule membrane protein 140; GMP-140; Leukocyte-endothelial cell adhesion molecule 3; LECAM3; Platelet activation dependent granule-external membrane protein; PADGEM; CD_antigen: CD62P
 Protein Family    P-selectin
 UniProt Gene Name    SELP [Similar Products]
 UniProt Synonym Gene Names    GMRP; GRMP; GMP-140; LECAM3; PADGEM [Similar Products]
 UniProt Entry Name    LYAM3_HUMAN
Section Bar
 NCBI Summary for SELP    This gene encodes a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. The membrane protein is a calcium-dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. Alternative splice variants may occur but are not well documented. [provided by RefSeq, Jul 2008]
Section Bar
 UniProt Comments for SELP    SELP: Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Belongs to the selectin/LECAM family.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 1q22-q25

Cellular Component: nucleoplasm; extracellular space; platelet alpha granule membrane; integral to plasma membrane; cytoplasm; plasma membrane; platelet dense granule membrane; external side of plasma membrane

Molecular Function: heparin binding; protein binding; lipopolysaccharide binding; glycosphingolipid binding; glycoprotein binding; calcium-dependent protein binding; sialic acid binding; fucose binding

Biological Process: platelet activation; positive regulation of leukocyte migration; response to lipopolysaccharide; defense response to Gram-negative bacterium; heterophilic cell adhesion; positive regulation of phosphoinositide 3-kinase cascade; leukocyte adhesion; platelet degranulation; regulation of integrin activation; leukocyte tethering or rolling; blood coagulation; cell adhesion; inflammatory response; leukocyte migration

Disease: Ige Responsiveness, Atopic
Table BarTOPTable Bar
 Research Articles on SELP    1. P-selectin Thr715Pro polymorphism is associated with myocardial infarction protection.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with SELP elisa kitPathways associated with SELP elisa kit
 Reference Product  PubMed Publications
 CRP elisa kit  >8 publications with SELP and CRP
 ITGAM elisa kit  >6 publications with SELP and ITGAM
 SELPLG elisa kit  >5 publications with SELP and SELPLG
 ITGB2 elisa kit  >4 publications with SELP and ITGB2
 CD63 elisa kit  >3 publications with SELP and CD63
 STAT6 elisa kit  >2 publications with SELP and STAT6
 Products by Pathway  Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 IL-3 Signaling Pathway antibodies  IL-3 Signaling Pathway Diagram
 IL4-mediated Signaling Events Pathway antibodies  IL4-mediated Signaling Events Pathway Diagram
 Malaria Pathway antibodies  Malaria Pathway Diagram
 Malaria Pathway antibodies  Malaria Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram
 Platelet Degranulation Pathway antibodies  Platelet Degranulation Pathway Diagram
Diseases associated with SELP elisa kitOrgans/Tissues associated with SELP elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >70 publications with SELP and Cardiovascular Diseases
 Inflammation Antibodies  >36 publications with SELP and Inflammation
 Heart Diseases Antibodies  >31 publications with SELP and Heart Diseases
 Myocardial Ischemia Antibodies  >27 publications with SELP and Myocardial Ischemia
 Atherosclerosis Antibodies  >23 publications with SELP and Atherosclerosis
 Thrombosis Antibodies  >17 publications with SELP and Thrombosis
 Disease Models, Animal Antibodies  >12 publications with SELP and Disease Models, Animal
 Myocardial Infarction Antibodies  >11 publications with SELP and Myocardial Infarction
 Necrosis Antibodies  >9 publications with SELP and Necrosis
 Stroke Antibodies  >8 publications with SELP and Stroke
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >112 publications with SELP and Blood
 Vascular Antibodies  >51 publications with SELP and Vascular
 Brain Antibodies  >15 publications with SELP and Brain
 Lung Antibodies  >12 publications with SELP and Lung
 Muscle Antibodies  >11 publications with SELP and Muscle
 Bone Antibodies  >7 publications with SELP and Bone
 Kidney Antibodies  >6 publications with SELP and Kidney
 Nerve Antibodies  >4 publications with SELP and Nerve
 Testis Antibodies  >4 publications with SELP and Testis
 Connective Tissue Antibodies  >3 publications with SELP and Connective Tissue
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions