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PACAP-27 elisa kit :: Human PACAP-27 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2510281
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

PACAP-27, ELISA Kit

★Popular Item★
 Also Known As   

Human PACAP-27 (Pituitary Adenylate Cyclase Activating Polypeptide 27) ELISA Kit

 Product Gene Name   

PACAP-27 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    102981
 3D Structure    ModBase 3D Structure for P41586
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 Species Reactivity    Human
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 Specificity    This kit recognizes natural and recombinant Human PACAP-27. No significant cross-reactivity or interference between Human PACAP-27 and analogues was observed.
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 Samples    Serum, plasma and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    0.313-20ng/mL
 Sensitivity    0.188ng/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PACAP-27 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PACAP-27 purchase    MBS2510281 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PACAP-27, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.313-20ng/mL in biological research samples containing PACAP-27, with an estimated sensitivity of 0.188ng/mL. The ELISA analytical biochemical technique of the MBS2510281 kit is based on PACAP-27 antibody-PACAP-27 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PACAP-27 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PACAP-27. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PACAP-27 elisa kit

   Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human PACAP-27 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PACAP-27. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PACAP-27 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PACAP-27, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PACAP-27. You can calculate the concentration of PACAP-27 in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of PACAP-27 elisa kit    PACAP-27 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2510281. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PACAP-27. It may not necessarily be applicable to this product.
 NCBI GI #    313851023
 NCBI GeneID    117
 NCBI Accession #    NP_001186566.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001199637.1 [Other Products]
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 UniProt Primary Accession #    P41586 [Other Products]
 UniProt Secondary Accession #    Q17S10; A8K1Y1; B7ZLA7; B8ZZK3 [Other Products]
 UniProt Related Accession #    P41586 [Other Products]
 Molecular Weight    46,957 Da
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 NCBI Official Full Name    pituitary adenylate cyclase-activating polypeptide type I receptor isoform 4
 NCBI Official Synonym Full Names    adenylate cyclase activating polypeptide 1 (pituitary) receptor type I
 NCBI Official Symbol    ADCYAP1R1 [Similar Products]
 NCBI Official Synonym Symbols   
PAC1; PAC1R; PACAPR; PACAPRI
[Similar Products]
 NCBI Protein Information    pituitary adenylate cyclase-activating polypeptide type I receptor; PACAP receptor 1; PACAP type I receptor; PACAP-R1; pituitary adenylate cyclase activating polypeptide 1 receptor type I Hiphop
 UniProt Protein Name    Pituitary adenylate cyclase-activating polypeptide type I receptor
 UniProt Gene Name    ADCYAP1R1 [Similar Products]
 UniProt Synonym Gene Names    PACAP type I receptor; PACAP-R-1; PACAP-R1 [Similar Products]
 UniProt Entry Name    PACR_HUMAN
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 NCBI Summary for PACAP-27    This gene encodes type I adenylate cyclase activating polypeptide receptor, which is a membrane-associated protein and shares significant homology with members of the glucagon/secretin receptor family. This receptor mediates diverse biological actions of adenylate cyclase activating polypeptide 1 and is positively coupled to adenylate cyclase. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Dec 2010]
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 UniProt Comments for PACAP-27    ADCYAP1R1: This is a receptor for PACAP-27 and PACAP-38. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. May regulate the release of adrenocorticotropin, luteinizing hormone, growth hormone, prolactin, epinephrine, and catecholamine. May play a role in spermatogenesis and sperm motility. Causes smooth muscle relaxation and secretion in the gastrointestinal tract. Belongs to the G-protein coupled receptor 2 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, GPCR; GPCR, family 2; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 7p14

Cellular Component: neuron projection; tight junction; cell surface; rough endoplasmic reticulum; integral to plasma membrane; plasma membrane; caveola; endosome; receptor complex

Molecular Function: neuropeptide binding; ADP-ribosylation factor binding; vasoactive intestinal polypeptide receptor activity; protein binding; receptor activity; adenylate cyclase binding

Biological Process: response to drug; development of primary female sexual characteristics; G-protein coupled receptor protein signaling pathway; cAMP-mediated signaling; cell surface receptor linked signal transduction; response to ethanol; nerve growth factor receptor signaling pathway; phospholipase C activation; adenylate cyclase activation; spermatogenesis; cell differentiation; multicellular organismal response to stress; response to estradiol stimulus; transmembrane receptor protein tyrosine kinase signaling pathway
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 Research Articles on PACAP-27    1. PAC1/PACAP receptor endocytosis contributes to ERK acktivation.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PACAP-27 elisa kitPathways associated with PACAP-27 elisa kit
 Reference Product  PubMed Publications
 ADCYAP1 elisa kit  >104 publications with PACAP-27 and ADCYAP1
 CRH elisa kit  >2 publications with PACAP-27 and CRH
 GHRH elisa kit  >2 publications with PACAP-27 and GHRH
 POMC elisa kit  >1 publications with PACAP-27 and POMC
 Products by Pathway  Pathway Diagram
 Activation Of TRKA Receptors Pathway antibodies  Activation Of TRKA Receptors Pathway Diagram
 Circadian Entrainment Pathway antibodies  Circadian Entrainment Pathway Diagram
 Circadian Entrainment Pathway antibodies  Circadian Entrainment Pathway Diagram
 Class B/2 (Secretin Family Receptors) Pathway antibodies  Class B/2 (Secretin Family Receptors) Pathway Diagram
 Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies  Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 G Alpha (s) Signalling Events Pathway antibodies  G Alpha (s) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class B Secretin-like Pathway antibodies  GPCRs, Class B Secretin-like Pathway Diagram
Diseases associated with PACAP-27 elisa kitOrgans/Tissues associated with PACAP-27 elisa kit
 Disease Name  Pubmed Publications
 Lung Neoplasms Antibodies  >6 publications with PACAP-27 and Lung Neoplasms
 Breast Neoplasms Antibodies  >6 publications with PACAP-27 and Breast Neoplasms
 Inflammation Antibodies  >5 publications with PACAP-27 and Inflammation
 Kidney Diseases Antibodies  >3 publications with PACAP-27 and Kidney Diseases
 Disease Models, Animal Antibodies  >2 publications with PACAP-27 and Disease Models, Animal
 Learning Disorders Antibodies  >2 publications with PACAP-27 and Learning Disorders
 Liver Neoplasms Antibodies  >2 publications with PACAP-27 and Liver Neoplasms
 Brain Injuries Antibodies  >2 publications with PACAP-27 and Brain Injuries
 Hyperplasia Antibodies  >2 publications with PACAP-27 and Hyperplasia
 Liver Diseases Antibodies  >2 publications with PACAP-27 and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >76 publications with PACAP-27 and Brain
 Nerve Antibodies  >28 publications with PACAP-27 and Nerve
 Pituitary Gland Antibodies  >26 publications with PACAP-27 and Pituitary Gland
 Blood Antibodies  >15 publications with PACAP-27 and Blood
 Ganglia Antibodies  >14 publications with PACAP-27 and Ganglia
 Muscle Antibodies  >12 publications with PACAP-27 and Muscle
 Adrenal Gland Antibodies  >11 publications with PACAP-27 and Adrenal Gland
 Lung Antibodies  >10 publications with PACAP-27 and Lung
 Kidney Antibodies  >6 publications with PACAP-27 and Kidney
 Intestine Antibodies  >5 publications with PACAP-27 and Intestine
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