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PAPPA2 elisa kit :: Human PAPPA2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2500517
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
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 Product Name   


★Popular Item★
 Also Known As   

Human PAPPA2 (Pappalysin 2) ELISA Kit

 Product Gene Name   

PAPPA2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for Q9BXP8
 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human PAPPA2. No significant cross-reactivity or interference between Human PAPPA2 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human PAPPA2 and all the analogues, therefore, cross reaction may still exist.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    0.313-20ng/mL
 Sensitivity    Min: 0.188ng/mL; Max: 20ng/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PAPPA2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PAPPA2 purchase    MBS2500517 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PAPPA2, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.313-20ng/mL in biological research samples containing PAPPA2, with an estimated sensitivity of Min: 0.188ng/mL; Max: 20ng/mL. The ELISA analytical biochemical technique of the MBS2500517 kit is based on PAPPA2 antibody-PAPPA2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PAPPA2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PAPPA2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PAPPA2 elisa kit

   Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PAPPA2 in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PAPPA2. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human PAPPA2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PAPPA2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PAPPA2. You can calculate the concentration of Human PAPPA2 in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of PAPPA2 elisa kit    PAPPA2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2500517. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PAPPA2. It may not necessarily be applicable to this product.
 NCBI GI #    116174738
 NCBI GeneID    60676
 NCBI Accession #    NP_064714.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_020318.2 [Other Products]
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 UniProt Primary Accession #    Q9BXP8 [Other Products]
 UniProt Secondary Accession #    Q96PH7; Q96PH8; Q9H4C9; A9Z1Y8 [Other Products]
 UniProt Related Accession #    Q9BXP8 [Other Products]
 Molecular Weight    92,136 Da
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 NCBI Official Full Name    pappalysin-2 isoform 1
 NCBI Official Synonym Full Names    pappalysin 2
 NCBI Official Symbol    PAPPA2 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    pappalysin-2; placenta-specific 3; pregnancy-associated plasma preproprotein-A2; pregnancy-associated plasma protein E1
 UniProt Protein Name    Pappalysin-2
 UniProt Synonym Protein Names   
Pregnancy-associated plasma protein A2; PAPP-A2; Pregnancy-associated plasma protein E1; PAPP-E
 Protein Family    Pappalysin
 UniProt Gene Name    PAPPA2 [Similar Products]
 UniProt Synonym Gene Names    PLAC3; PAPP-A2; PAPP-E [Similar Products]
 UniProt Entry Name    PAPP2_HUMAN
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 NCBI Summary for PAPPA2    This gene encodes a member of the pappalysin family of metzincin metalloproteinases. The encoded protein cleaves insulin-like growth factor-binding protein 5 and is thought to be a local regulator of insulin-like growth factor (IGF) bioavailability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010]
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 UniProt Comments for PAPPA2    PAPPA2: Metalloproteinase which specifically cleaves IGFBP-5. Shows limited proteolysis toward IGFBP-3. Belongs to the peptidase M43B family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; EC 3.4.24.-; Protease; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q25.2

Cellular Component: membrane; extracellular region; intracellular

Molecular Function: metallopeptidase activity; zinc ion binding

Biological Process: cellular protein metabolic process; regulation of cell growth; cell differentiation; proteolysis
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 Research Articles on PAPPA2    1. PAPPA2 may be upregulated in severe pre-eclampsia and, functionally, this may be mediated via increased placental hypoxia known to occur with this pregnancy disorder.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PAPPA2 elisa kitPathways associated with PAPPA2 elisa kit
 Reference Product  PubMed Publications
 IGFBP5 elisa kit  >7 publications with PAPPA2 and IGFBP5
 IGFBP4 elisa kit  >2 publications with PAPPA2 and IGFBP4
 Products by Pathway  Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway antibodies  Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway Diagram
Diseases associated with PAPPA2 elisa kitOrgans/Tissues associated with PAPPA2 elisa kit
 Disease Name  Pubmed Publications
 Neurobehavioral Manifestations Antibodies  >4 publications with PAPPA2 and Neurobehavioral Manifestations
 Insulin Resistance Antibodies  >2 publications with PAPPA2 and Insulin Resistance
 Cardiovascular Diseases Antibodies  >2 publications with PAPPA2 and Cardiovascular Diseases
 Necrosis Antibodies  >2 publications with PAPPA2 and Necrosis
 Weight Loss Antibodies  >1 publications with PAPPA2 and Weight Loss
 Anemia Antibodies  >1 publications with PAPPA2 and Anemia
 Pain Antibodies  >1 publications with PAPPA2 and Pain
 Organ/Tissue Name  Pubmed Publications
 Placenta Antibodies  >13 publications with PAPPA2 and Placenta
 Bone Antibodies  >5 publications with PAPPA2 and Bone
 Liver Antibodies  >2 publications with PAPPA2 and Liver
 Heart Antibodies  >2 publications with PAPPA2 and Heart
 Brain Antibodies  >2 publications with PAPPA2 and Brain
 Uterus Antibodies  >2 publications with PAPPA2 and Uterus
 Muscle Antibodies  >1 publications with PAPPA2 and Muscle
 Lung Antibodies  >1 publications with PAPPA2 and Lung
 Pancreas Antibodies  >1 publications with PAPPA2 and Pancreas
 Kidney Antibodies  >1 publications with PAPPA2 and Kidney
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