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PDGF-AB elisa kit :: Human PDGF-AB ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2512188
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

PDGF-AB, ELISA Kit

★Popular Item★
 Also Known As   

Human PDGF-AB (Platelet Derived Growth Factor AB) ELISA Kit

 Product Gene Name   

PDGF-AB elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    173430
 3D Structure    ModBase 3D Structure for P04085
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human PDGF-AB. No significant cross-reactivity or interference between Human PDGF-AB and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human PDGF-AB and all the analogues, therefore, cross reaction may still exist.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    31.25--2000pg/mL
 Sensitivity    18.75pg/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PDGF-AB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PDGF-AB purchase    MBS2512188 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PDGF-AB, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25--2000pg/mL in biological research samples containing PDGF-AB, with an estimated sensitivity of 18.75pg/mL. The ELISA analytical biochemical technique of the MBS2512188 kit is based on PDGF-AB antibody-PDGF-AB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PDGF-AB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PDGF-AB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PDGF-AB elisa kit

   Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PDGF-AB in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PDGF-AB. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human PDGF-AB and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PDGF-AB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PDGF-AB. You can calculate the concentration of Human PDGF-AB in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of PDGF-AB elisa kit    PDGF-AB elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2512188. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PDGF-AB. It may not necessarily be applicable to this product.
 NCBI GI #    15208656
 NCBI GeneID    5154
 NCBI Accession #    NP_148983.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_033023.4 [Other Products]
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 UniProt Primary Accession #    P04085 [Other Products]
 UniProt Secondary Accession #    B5BU73 [Other Products]
 UniProt Related Accession #    P04085 [Other Products]
 Molecular Weight    22,253 Da
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 NCBI Official Full Name    platelet-derived growth factor subunit A isoform 2 preproprotein
 NCBI Official Synonym Full Names    platelet-derived growth factor alpha polypeptide
 NCBI Official Symbol    PDGFA [Similar Products]
 NCBI Official Synonym Symbols   
PDGF1; PDGF-A
[Similar Products]
 NCBI Protein Information    platelet-derived growth factor subunit A; PDGF A-chain; PDGF subunit A; PDGF-1; platelet-derived growth factor A chain; platelet-derived growth factor alpha chain; platelet-derived growth factor alpha isoform 2 preproprotein
 UniProt Protein Name    Platelet-derived growth factor subunit A
 UniProt Synonym Protein Names   
PDGF-1; Platelet-derived growth factor A chain; Platelet-derived growth factor alpha polypeptide
 UniProt Gene Name    PDGFA [Similar Products]
 UniProt Synonym Gene Names    PDGF1; PDGF subunit A [Similar Products]
 UniProt Entry Name    PDGFA_HUMAN
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 NCBI Summary for PDGF-AB    The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer or as a heterodimer with the platelet-derived growth factor beta polypeptide, where the dimers are connected by disulfide bonds. Studies using knockout mice have shown cellular defects in oligodendrocytes, alveolar smooth muscle cells, and Leydig cells in the testis; knockout mice die either as embryos or shortly after birth. Two splice variants have been identified for this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for PDGF-AB    PDGFA: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Cytokine; Secreted, signal peptide; Cell cycle regulation

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: Golgi membrane; extracellular space; cell surface; microvillus; endoplasmic reticulum lumen; extracellular region

Molecular Function: collagen binding; protein binding; protein homodimerization activity; growth factor activity; protein heterodimerization activity; platelet-derived growth factor binding; platelet-derived growth factor receptor binding

Biological Process: skin development; extracellular matrix organization and biogenesis; wound healing; nerve growth factor receptor signaling pathway; negative chemotaxis; platelet-derived growth factor receptor signaling pathway; cell projection biogenesis; response to estradiol stimulus; positive regulation of MAP kinase activity; positive regulation of fibroblast proliferation; regulation of smooth muscle cell migration; platelet degranulation; hair follicle development; positive regulation of MAPKKK cascade; cell-cell signaling; transforming growth factor beta receptor signaling pathway; positive regulation of mesenchymal cell proliferation; positive regulation of cell proliferation; response to wounding; angiogenesis; inner ear development; regulation of peptidyl-tyrosine phosphorylation; response to drug; epidermal growth factor receptor signaling pathway; platelet activation; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; response to retinoic acid; response to inorganic substance; negative regulation of phosphatidylinositol biosynthetic process; positive regulation of protein amino acid autophosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; cell activation; organ morphogenesis; regulation of actin cytoskeleton organization and biogenesis; positive regulation of cell division; response to hypoxia; innate immune response; blood coagulation; actin cytoskeleton organization and biogenesis; alveolus development; positive regulation of DNA replication; positive regulation of cell migration
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 Research Articles on PDGF-AB    1. PDGFA is a SOX11 direct target gene upregulated in MCL cells whose inhibition impaired SOX11-enhanced in vitro angiogenic effects on endothelial cells
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PDGF-AB elisa kitPathways associated with PDGF-AB elisa kit
 Reference Product  PubMed Publications
 PDGFRA elisa kit  >44 publications with PDGF-AB and PDGFRA
 PDGFB elisa kit  >30 publications with PDGF-AB and PDGFB
 PDGFRB elisa kit  >22 publications with PDGF-AB and PDGFRB
 EGFR elisa kit  >10 publications with PDGF-AB and EGFR
 FLT1 elisa kit  >2 publications with PDGF-AB and FLT1
 FGF1 elisa kit  >2 publications with PDGF-AB and FGF1
 SP1 elisa kit  >1 publications with PDGF-AB and SP1
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Diseases associated with PDGF-AB elisa kitOrgans/Tissues associated with PDGF-AB elisa kit
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >23 publications with PDGF-AB and Carcinoma
 Adenocarcinoma Antibodies  >15 publications with PDGF-AB and Adenocarcinoma
 Inflammation Antibodies  >11 publications with PDGF-AB and Inflammation
 Fibrosis Antibodies  >9 publications with PDGF-AB and Fibrosis
 Disease Models, Animal Antibodies  >8 publications with PDGF-AB and Disease Models, Animal
 Brain Diseases Antibodies  >7 publications with PDGF-AB and Brain Diseases
 Cell Transformation, Neoplastic Antibodies  >7 publications with PDGF-AB and Cell Transformation, Neoplastic
 Neoplasms, Experimental Antibodies  >7 publications with PDGF-AB and Neoplasms, Experimental
 Mesothelioma Antibodies  >4 publications with PDGF-AB and Mesothelioma
 Pancreatic Neoplasms Antibodies  >4 publications with PDGF-AB and Pancreatic Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >13 publications with PDGF-AB and Liver
 Brain Antibodies  >10 publications with PDGF-AB and Brain
 Lung Antibodies  >10 publications with PDGF-AB and Lung
 Embryonic Tissue Antibodies  >9 publications with PDGF-AB and Embryonic Tissue
 Connective Tissue Antibodies  >7 publications with PDGF-AB and Connective Tissue
 Kidney Antibodies  >6 publications with PDGF-AB and Kidney
 Testis Antibodies  >5 publications with PDGF-AB and Testis
 Mouth Antibodies  >5 publications with PDGF-AB and Mouth
 Ovary Antibodies  >5 publications with PDGF-AB and Ovary
 Intestine Antibodies  >4 publications with PDGF-AB and Intestine
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